Human Anti-CEACAM5 Recombinant Antibody (TAB-415LC)
CAT#: TAB-415LC
This product is a recombinant Human antibody that recognizes Carcinoembryonic antigen. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19758/1660_G5_2_selected.jpg
Subcellular Location
Figure 2 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in a subset of leukocytes.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11041/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Gradient
Gradient: Ascending
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21/19_A_7_3.jpg
Normal Tissue
Figure 4 Rectum
Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21/19_A_6_3.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105388-CEACAM5
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Specifications
- Immunogen
- CHO cells transfected with human CEA
- Host Species
- Human
- Derivation
- Humanized
- Type
- Human IgG1
- Specificity
- Human CEACAM5
- Species Reactivity
- Human
- Applications
- ELISA, FC, Inhib
- Related Disease
- Epithelial tumor
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- CEACAM5; carcinoembryonic antigen-related cell adhesion molecule 5; CEA; CD66e; meconium antigen 100;
- Gene ID
- 1048
- UniProt ID
- A0A024R0K5
REVIEWS AND Q&AS
CITATIONS
RESOURCES
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High Purity
The consistency and high purity of TAB-415LC are evident in every experiment. The recombinant nature ensures that batch-to-batch consistency is maintained, which is crucial for reproducibility.
Excellent for Flow Cytometry
I primarily use TAB-415LC for flow cytometry to sort CEACAM5-positive cells. The antibody binds specifically and provides sharp, distinguishable signals. It's been a cornerstone for my cellular analysis.
Reliable ELISA Results
In our ELISA assays, TAB-415LC has provided reliable detection and quantification of CEACAM5. The detailed protocol offered by the manufacturer made it easy to achieve consistent results.
Q&As
-
What applications can TAB-415LC be used for in the lab?
A: TAB-415LC can be used in various applications including Western blotting, immunoprecipitation, flow cytometry, immunofluorescence, and enzyme-linked immunosorbent assay (ELISA), making it versatile for studying CEACAM5 expression and function.
-
Can TAB-415LC be used for flow cytometry?
A: Absolutely, TAB-415LC is suitable for use in flow cytometry for detecting CEACAM5 expression on the cell surface, providing detailed analysis of cell populations based on antigen presence.
-
How should I reconstitute the lyophilized antibody?
A: The lyophilized TAB-415LC should be reconstituted in sterile distilled water or a buffer recommended by the manufacturer's datasheet, typically at a concentration ensuring optimum functionality.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| HPAB-0243-FY-F(E) | Human Anti-CEACAM5 Recombinant Antibody Fab Fragment (HPAB-0243-FY-F(E)) | ELISA | Chimeric (mouse/human) Fab |
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