Hi-Affi™ Rabbit Anti-CHEK2 Recombinant Antibody (clone DS689AB) (CAT#: MOR-0689)
Recombinant Rabbit anti-CHEK2 monoclonal antibody for Dot, IP, WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/if_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/if_selected.jpg
Figure 2 Skin
(Keratinocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Langerhans Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_B_9_1.jpg
(Keratinocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Langerhans Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_B_9_1.jpg
Figure 3 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_7_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_7_8.jpg
Figure 4 Colon
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_7_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_7_3.jpg
Figure 5 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_8_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_8_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_5_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_5_6.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000183765-CHEK2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000183765-CHEK2
Specifications
- Immunogen
- A synthetic phospho-peptide corresponding to residues surrounding Threonine 68 of human Chk2.
- Host Species
- Rabbit
- Derivation
- Phage display library
- Type
- Rabbit IgG
- Specificity
- Human CHEK2
- Species Reactivity
- Human
- Clone
- DS689AB
- Applications
- DB, IP, WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Tissue culture supernatant
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Checkpoint Kinase 2; CHK2 Checkpoint Homolog; Cds1 Homolog; HuCds1; RAD53; HCds1; CDS1; CHK2; CHK2 (Checkpoint, S.Pombe) Homolog; CHK2 Checkpoint Homolog (S. Pombe); Checkpoint-Like Protein CHK2; EC 2.7.11.1; EC 2.7.11; PP1425; LFS2
- Gene ID
- 11200
- UniProt ID
- O96017
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4034z | Mouse Anti-CHEK2 Recombinant Antibody (clone 30H3) | WB, ELISA, IF, IHC | Mouse IgG2a, κ |
| MOB-0642MZ | Recombinant Mouse Anti-Human CHEK2 Antibody (clone 9G23) | WB | Mouse antibody |
| VS3-QX235 | Mouse Anti-CHEK2 Recombinant Antibody (clone 4B1-C5-B3) | WB, IHC, ICC, FC, ELISA | Mouse IgG2b |
| VS3-CJ199 | Rabbit Anti-CHEK2 Recombinant Antibody (VS3-CJ199) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| VS3-WK2072 | Rabbit Anti-CHEK2 (pT68) Recombinant Antibody (clone R23-2L-9) | WB, IP | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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