Hi-Affi™ Rabbit Anti-CHEK2 Recombinant Antibody (clone TH26DS)
CAT#: MOR-4518
Recombinant Rabbit anti-CHEK2 monoclonal antibody for WB, IF, ICC, FCM, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/if_selected.jpg
Normal Tissue
Figure 2 Skin
Keratinocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Langerhans Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_B_9_1.jpg
Normal Tissue
Figure 3 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_7_8.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_5_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000183765-CHEK2
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Specifications
- Immunogen
- Peptide corresponding to Human CHEK2 (aa 386-392)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CHEK2
- Species Reactivity
- Human
- Clone
- TH26DS
- Applications
- WB, IF, ICC, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, pH 7.2
- Preservative
- 0.09% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Flow Cytometry (2-4 µg per 10^6 cells), Immunocytochemistry (2 µg/ml), Immunofluorescence (2 µg/ml) and Western Blot (1-2 µg/ml).
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "CHEK2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0122MZ | Chicken Anti-CHEK2 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0689 | Hi-Affi™ Rabbit Anti-CHEK2 Recombinant Antibody (clone DS689AB) | DB, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0420F | Mouse Anti-CHEK2 Recombinant Antibody (ZG-0420F) | WB, IHC, IF, ELISA | Mouse IgG |
| VS3-CJ199 | Rabbit Anti-CHEK2 Recombinant Antibody (VS3-CJ199) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| VS3-FY377 | Recombinant Rabbit Anti-CHEK2 Antibody (clone R04-8E5) | WB, ICC, IF, IP | Rabbit IgG |
| VS3-FY378 | Recombinant Rabbit Anti-CHEK2 Antibody (clone R06-1B4) | WB, ICC, IF, IP | Rabbit IgG |
| VS13-YC218 | Rabbit Anti-CHEK2 Recombinant Antibody (VS13-YC218) | WB, ICC, IF, IHC-P, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC455 | AbPlus™ Anti-CHEK2 Magnetic Beads (VS-0724-YC455) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY121 | Mouse Anti-NHP CHEK2 Recombinant Antibody (clone 1C12B8) | WB | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY446 | Anti-CHEK2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1430 | Anti-Mouse CHEK2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1431 | Anti-Canine CHEK2 Immunohistochemistry Kit | IHC |
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