Recombinant Human Anti-CHIKV E2 Antibody (CHK265) (CAT#: PABL-430)
Recombinant Human Antibody (CHK265) is capable of binding to CHIKV E2, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-CHIKV E2 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-CHIKV E2 proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody bound to CHIKV E2 B domain provided an explanation for its neutralizing activity. Binding to the B domain was associated with repositioning of the A domain of E2 that enabled cross-linking of neighboring spikes.
Specific Inquiry
IB
Figure 1 Production of anti-CHIKV-E2 mAb-secreting hybridomas.
For immunoblotting, the protein was transferred from the gel onto a PVDF membrane and probed with commercial mAbs (Chk265), followed by incubation with HRP-conjugated anti-mouse IgG. Lane M indicates a protein marker (kDa).
Kim, J., Yang, J., Kim, Y. B., Lee, H. J., Kim, S., & Poo, H. (2019). Development of a Specific CHIKV-E2 Monoclonal Antibody for Chikungunya Diagnosis. Virologica Sinica, 34(5), 563-571.
ELISA
Figure 2 Comparison of the binding affinities of the anti-CHIKV-E2 mAbs to the CHIKV-E2 protein.
As a spiking experiment, ELISA plates were coated with CHIKV-E2 protein (5 μg/mL) in PBS containing 1% human serum, blocked, and treated with the anti-CHIKV-E2 mAbs (10 μg/mL), followed by incubation with HRP-conjugated anti-mouse IgG. Commercial antiCHIKV-E2 mAbs (Chk265 and 16A12) were used for comparison.
Kim, J., Yang, J., Kim, Y. B., Lee, H. J., Kim, S., & Poo, H. (2019). Development of a Specific CHIKV-E2 Monoclonal Antibody for Chikungunya Diagnosis. Virologica Sinica, 34(5), 563-571.
BL
Figure 3 Specificity of the anti-CHIKV E2 mAbs.
After blocking with 5% skim milk in PBS, the plates were incubated with the Chk265 mAb (1 μg/mL), followed by incubation with HRP-conjugated anti-mouse IgG.
Kim, J., Yang, J., Kim, Y. B., Lee, H. J., Kim, S., & Poo, H. (2019). Development of a Specific CHIKV-E2 Monoclonal Antibody for Chikungunya Diagnosis. Virologica Sinica, 34(5), 563-571.
Specifications
- Immunogen
- Chikungunya virus E2 glycoprotein
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native CHIKV E2
- Species Reactivity
- CHIKV
- Clone
- CHK265
- Applications
- IB, ELISA, BL
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- This antibody has been reported for use in Immunoblotting, Enzyme-Linked Immunosorbent Assay and Blocking.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-431 | Recombinant Mouse Anti-CHIKV E2 Antibody (CHK9) | Neut, BL | IgG |
| MOB-152CQ | Mouse Anti-CHIKV E2 Antibody (CHK265) | Neut, ELISA, IF, FC | Mouse IgG1, λ |
| HPAB-S0184-YC | Human Anti-CHIKV E2 Recombinant Antibody (clone ch-CHK-152) | Neut, FRNT, FuncS | Human IgG |
| HPAB-S0185-YC | Human Anti-CHIKV E2 Recombinant Antibody (clone ch-CHK-152 N297Q) | Neut, FRNT, FuncS | Human IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-427 | Recombinant Human Anti-CHIKV E2 Antibody Fab Fragment (CHK265) | Neut | Fab |
| PFBL-428 | Recombinant Mouse Anti-CHIKV E2 Antibody Fab Fragment (CHK9) | Neut, BL | Fab |
| HPAB-S0184-YC-F(E) | Human Anti-CHIKV E2 Recombinant Antibody (clone ch-CHK-152); Fab Fragment | Neut, FRNT, FuncS | Human Fab |
| HPAB-S0185-YC-F(E) | Human Anti-CHIKV E2 Recombinant Antibody (clone ch-CHK-152 N297Q); Fab Fragment | Neut, FRNT, FuncS | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-427 | Recombinant Human Anti-CHIKV E2 Antibody scFv Fragment (CHK265) | Neut | scFv |
| PSBL-428 | Recombinant Mouse Anti-CHIKV E2 Antibody scFv Fragment (CHK9) | Neut, BL | scFv |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1158LC-S(P) | Anti-CHIKV E2 Recombinant Antibody scFv Fragment (3C3) | ELISA, WB, IF | Mouse Antibody |
| MRO-1159LC-S(P) | Anti-CHIKV E2 Recombinant Antibody scFv Fragment (3E4) | ELISA, WB, IF | Mouse Antibody |
| MRO-1158LC-F(E) | Anti-CHIKV E2 Recombinant Antibody Fab Fragment (3C3) | ELISA, WB, IF | Mouse Antibody |
| MRO-1159LC-F(E) | Anti-CHIKV E2 Recombinant Antibody Fab Fragment (3E4) | ELISA, WB, IF | Mouse Antibody |
| MRO-1160LC-F(E) | Anti-CHIKV E2 Recombinant Antibody Fab Fragment (8A4) | ELISA, WB, IF | Mouse Antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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