Recombinant Mouse Anti-CHIKV E2 glycoprotein Antibody (5F-10) (CAT#: PABL-040)
Recombinant Mouse Antibody (5F-10) is capable of binding to CHIKV E2 glycoprotein, expressed in Chinese Hamster Ovary cells (CHO). This antibody fixes the B domain rigidly to the surface of the virus, blocking exposure of the fusion loop on glycoprotein E1, and therefore preventing the virus from becoming fusogenic.
Specific Inquiry
IF
Figure 1 5F10 mAbs bind and neutralize CHIKV in vitro.
Purified mAbs bind the CHIKV11 isolate as assessed by immunofluorescence binding assay. Infected HEK293T cells probed with 5F10. All mAbs were used at 1 μg/ml.
Warter, L., Lee, C. Y., Thiagarajan, R., Grandadam, M., Lebecque, S., Lin, R. T., ... & Wang, C. I. (2011). Chikungunya virus envelope-specific human monoclonal antibodies with broad neutralization potency. The Journal of Immunology, 186(5), 3258-3264.
Neut
Figure 2 Recombinant mAbs are as potent as the corresponding purified mAbs in neutralizing the CHIKV11 isolate.
mAbs were tested in a plaque reduction neutralization test across a concentration range from 100 pg/ml to 200 μg/ml. The means and SD from three independent matched experiments, as well as the nonlinear regression fitting curves, are shown. Original magnification ×100.
Warter, L., Lee, C. Y., Thiagarajan, R., Grandadam, M., Lebecque, S., Lin, R. T., ... & Wang, C. I. (2011). Chikungunya virus envelope-specific human monoclonal antibodies with broad neutralization potency. The Journal of Immunology, 186(5), 3258-3264.
IP
Figure 3 5F10 is specific for the E2 protein.
CHIKV11-infected cell lysate was immunoprecipitated by 3E4, 5F10, 8B10, or irrelevant IgG1 mAbs.
Warter, L., Lee, C. Y., Thiagarajan, R., Grandadam, M., Lebecque, S., Lin, R. T., ... & Wang, C. I. (2011). Chikungunya virus envelope-specific human monoclonal antibodies with broad neutralization potency. The Journal of Immunology, 186(5), 3258-3264.
Neut
Figure 4 5F10 mAb neutralizes several CHIKV isolates, as well as the ONNV.
Percent virus neutralization by 100 μg/ml (open symbols) and 100 ng/ml (black symbols) of 5F10 mAb, as measured by a plaque reduction neutralization test.
Warter, L., Lee, C. Y., Thiagarajan, R., Grandadam, M., Lebecque, S., Lin, R. T., ... & Wang, C. I. (2011). Chikungunya virus envelope-specific human monoclonal antibodies with broad neutralization potency. The Journal of Immunology, 186(5), 3258-3264.
Specifications
- Immunogen
- Chikungunya virus E2 glycoprotein
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native CHIKV E2
- Species Reactivity
- CHIKV
- Clone
- 5F-10
- Applications
- IF, Neut, IP
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- This antibody has been reported for use in Immunofluorescence, Neutralization and Immunoprecipitation.
Target
- Alternative Names
- E2; E2 glycoprotein; CHIKV; chikungunya virus
- Gene ID
- 956308
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-118 | Recombinant Rat Anti-HCV E2 Antibody (3/11) | WB, ELISA, Neut, FuncS | IgG |
| PABL-119 | Recombinant Human Anti-HCV E2 Antibody (HC33.1) | Neut, FuncS | IgG |
| PABL-120 | Recombinant Human Anti-HCV E2 Antibody (HC84-1) | WB, ELISA, Neut, FuncS | IgG |
| PABL-121 | Recombinant Mouse Anti-HCV E2 Antibody (HC84-27) | Neut, FuncS | IgG |
| PABL-122 | Recombinant Mouse Anti-HCV E2 Antibody (mAb12) | WB, ELISA, FuncS | IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-040 | Recombinant Mouse Anti-CHIKV E2 glycoprotein Antibody scFv Fragment (5F-10) | Neut, FuncS | scFv |
| PSBL-357 | Recombinant Human Anti-VEEV E2 Antibody scFv Fragment (F5) | WB, ELISA, Neut, FuncS | scFv |
| PFBZ-180 | Recombinant Mouse Anti-HCV E2 Antibody Fab Fragment (mAb#8) | Neut | Fab |
| PFBC-184 | Recombinant Chimeric (Human/Mouse) Anti-HCV glycoprotein E2 Antibody Fab Fragment (mAb1:7) | Neut | Fab |
| HPAB-2105-FY-S(P) | Human Anti-E2 Recombinant Antibody (clone D12); scFv Fragment | ELISA | Human scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-119 | Recombinant Human Anti-HCV E2 Antibody Fab Fragment (HC33.1) | Neut, FuncS | Fab |
| PFBL-120 | Recombinant Human Anti-HCV E2 Antibody Fab Fragment (HC84-1) | WB, ELISA, Neut, FuncS | Fab |
| PFBL-121 | Recombinant Mouse Anti-HCV E2 Antibody Fab Fragment (HC84-27) | Neut, FuncS | Fab |
| PFBL-122 | Recombinant Mouse Anti-HCV E2 Antibody Fab Fragment (mAb12) | WB, ELISA, FuncS | Fab |
| PFBL-123 | Recombinant Mouse Anti-HCV E2 Antibody Fab Fragment (mAb8) | WB, Neut, FuncS | Fab |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-019 | Recombinant Anti-HCV E2 VHH Single Domain Antibody (D03) | Inhib, FuncS | Llama VHH |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-020CT | Human Anti-HCV E2 Recombinant Antibody (AP33) | ELISA, IP, Neut, FC, WB | Human antibody |
| TAB-238CT | Anti-HCV E2 Recombinant Antibody (Y47F) | ELISA, WB | Human antibody |
| TAB-239CT | Anti-HCV E2 Recombinant Antibody (Y47W) | ELISA, WB | Human antibody |
| TAB-238CT-F(E) | Anti-HCV E2 Recombinant Antibody Fab Fragment (Y47F) | ELISA, WB | Human antibody |
| TAB-239CT-F(E) | Anti-HCV E2 Recombinant Antibody Fab Fragment (Y47W) | ELISA, WB | Human antibody |
MHC Tetramer for Virology
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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