Hi-Affi™ Rabbit Anti-CISH Recombinant Antibody (clone TH3DS)
CAT#: MOR-4495
Recombinant Rabbit anti-CISH monoclonal antibody for WB, IF, ICC, FCM, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules & cytokinetic bridge.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40812/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40812/114569_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40812/114569_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40812/114569_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40812/114569_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40812/114569_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40812/114569_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enriched (HDLM-2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000114737-CISH
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Specifications
- Immunogen
- Protein corresponding to Human CISH (aa 1-258)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CISH
- Species Reactivity
- Human
- Clone
- TH3DS
- Applications
- WB, IF, ICC, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, pH 7.2
- Preservative
- 0.09% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Flow Cytometry (2-4 µg per 10^6 cells), Immunocytochemistry (1-2 µg/ml), Immunofluorescence (1-2 µg/ml), Western Blot (1-2 µg/ml).
Target
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Efficacious at Different pHs
Stability was tested at various pH values, and it remained active and specific throughout the range.
Friendly Aliquoting for Users
Aliquotting was simple, and the smaller aliquots were stable for a long time without degradation.
Suitable for Applications Sensitive to Light
Wrapped in foil, this antibody performed well in light-sensitive assays and showed no signs of degradation.
Q&As
-
Can antibody solutions be reused in immunoassays?
A: Reusing antibody solutions is generally discouraged as it may compromise consistency and reliability.
-
Which type of secondary antibody is suitable for my needs?
A: Select a secondary antibody that recognizes the host species and subclass of the primary antibody for your experiments.
-
Are monoclonal antibodies more specific than polyclonal antibodies?
A: Monoclonal antibodies often provide greater specificity and consistency, while polyclonal antibodies may offer higher sensitivity due to broader epitope recognition.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# MOR-4495, RRID: AB_3111515)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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