Human Anti-CKM Recombinant Antibody (clone MAK33) (CAT#: PABW-026)
Recombinant Human Antibody (MAK33) is capable of binding to CK-MM, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-CK-MM mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-CK-MM mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Specific Inquiry
Figure 1 Most stable complex structure from (a) NMR-guided and (b) unguided simulations of MAK33 VL with EGCG.
Root mean square fluctuations (RMSF) for each residue according to MD simulations starting from complexes obtained from (c) NMR-guided and (d) unguided docking simulations.
Hora, M., Carballo-Pacheco, M., Weber, B., Morris, V. K., Wittkopf, A., Buchner, J., ... & Reif, B. (2017). Epigallocatechin-3-gallate preferentially induces aggregation of amyloidogenic immunoglobulin light chains. Scientific reports, 7, 41515.
Figure 2 Stable and transient overexpression of p58(+), but not of p58(–), alongside transient co-expression of HCs and LCs of the mouse monoclonal MAK33 increased the relative yield of secreted antibody.
(A and B) Immunoblot detection of the indicated proteins in cell extracts and growth medium prepared on the indicated day after co-transfection of the indicated cell lines with plasmids encoding MAK33 HCs and LCs and, in the case of 'p58(+) transient, with plasmid encoding p58 with its ER leader sequence. (C) Quantification of immunoblot data for the indicated cell type over 4 days post-transfection as a ratio of medium/cell HC band density. Results are means±S.D. (n=3). *P<0.05 between control and transfected values on the indicated day of culture. trs, transient.
Roobol, A., Roobol, J., Bastide, A., Knight, J. R., Willis, A. E., & Smales, C. M. (2015). p58IPK is an inhibitor of the eIF2α kinase GCN2 and its localization and expression underpin protein synthesis and ER processing capacity. Biochemical Journal, 465(2), 213-225.
Specifications
- Immunogen
- Human creatine kinase, muscle
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human CKM
- Species Reactivity
- Human
- Clone
- MAK33
- Applications
- WB, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The CKM antibody has been reported in applications of NMR, Western Blot.
NMR: Backbone assignments for MAK33 VL WT have been reported previously25. Heteronuclear single quantum coherence (HSQC) experiments were recorded using a protein concentration of 50 μM at a temperature of 25 °C. All NMR experiments were performed using a Bruker Avance III spectrometer (Bruker BioSpin, Karlsruhe) operating at a 1H Larmor frequency of 500 MHz.
Target
- Alternative Names
- CKM; creatine kinase, muscle; CKMM; M-CK; creatine kinase M-type; creatine kinase M chain;
- Gene ID
- 1158
- UniProt ID
- B2R892
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone MAK33"
See other products for "CKM"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-213 | Recombinant Anti-human CKM Antibody | WB, FC, FuncS | IgG |
| PABL-377 | Recombinant Mouse Anti-CKM Antibody (MAK33) | WB, ELISA, FuncS | IgG |
| MOB-0045MZ | Recombinant Mouse Anti-Human Creatine Kinase MM Antibody (clone A027-17186) | ELISA | Mouse antibody |
| VS3-WK719 | Mouse Anti-CKM Recombinant Antibody (clone K68t3) | WB, ELISA | Mouse IgG |
| VS3-WK720 | Mouse Anti-CKM Recombinant Antibody (clone K69v5) | WB, ELISA | Mouse IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-213-F(E) | Recombinant Anti-human CKM Antibody Fab Fragment | ELISA, Nert, FuncS | Fab |
| MHH-213-F(E) | Recombinant Human Anti-human CKM Antibody Fab Fragment | ELISA, WB, FuncS | Fab |
| PFBL-377 | Recombinant Mouse Anti-CKM Antibody Fab Fragment (MAK33) | WB, ELISA, FuncS | Fab |
| PFBW-026 | Human Anti-CKM Recombinant Antibody (clone MAK33); Fab Fragment | WB, FuncS | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-213-S(P) | Recombinant Anti-human CKM Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| MHH-213-S(P) | Recombinant Human Anti-human CKM Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| PSBL-377 | Recombinant Mouse Anti-CKM Antibody scFv Fragment (MAK33) | WB, ELISA, FuncS | scFv |
| PSBW-026 | Human Anti-CKM Recombinant Antibody (clone MAK33); scFv Fragment | WB, FuncS | Human scFv |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for PABW-026. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.