Human Anti-CKM Recombinant Antibody (clone MAK33) (CAT#: PABW-026)
Recombinant Human Antibody (MAK33) is capable of binding to CK-MM, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-CK-MM mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-CK-MM mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
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Figure 1 Most stable complex structure from (a) NMR-guided and (b) unguided simulations of MAK33 VL with EGCG.
Root mean square fluctuations (RMSF) for each residue according to MD simulations starting from complexes obtained from (c) NMR-guided and (d) unguided docking simulations.
Hora, M., Carballo-Pacheco, M., Weber, B., Morris, V. K., Wittkopf, A., Buchner, J., ... & Reif, B. (2017). Epigallocatechin-3-gallate preferentially induces aggregation of amyloidogenic immunoglobulin light chains. Scientific reports, 7, 41515.
Specifications
- Immunogen
- Human creatine kinase, muscle
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human CKM
- Species Reactivity
- Human
- Clone
- MAK33
- Applications
- WB, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The CKM antibody has been reported in applications of NMR, Western Blot.
NMR: Backbone assignments for MAK33 VL WT have been reported previously25. Heteronuclear single quantum coherence (HSQC) experiments were recorded using a protein concentration of 50 μM at a temperature of 25 °C. All NMR experiments were performed using a Bruker Avance III spectrometer (Bruker BioSpin, Karlsruhe) operating at a 1H Larmor frequency of 500 MHz.
Target
- Alternative Names
- CKM; creatine kinase, muscle; CKMM; M-CK; creatine kinase M-type; creatine kinase M chain;
- Gene ID
- 1158
- UniProt ID
- B2R892
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
Fab Antibody Products
ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-213 | Recombinant Anti-human CKM Antibody | WB, FC, FuncS | IgG |
| MOB-0340CT | Recombinant Mouse anti-Human CTSL1 Monoclonal antibody (DQM44/2) | I-ELISA, IHC-Fr, WB | |
| MOB-0342CT | Recombinant Mouse anti-Human CTSL2 Monoclonal antibody (CW66-2) | IHC-P, WB | |
| VS3-WK719 | Mouse Anti-CKM Recombinant Antibody (clone K68t3) | WB, ELISA | Mouse IgG |
| VS3-WK720 | Mouse Anti-CKM Recombinant Antibody (clone K69v5) | WB, ELISA | Mouse IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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