Mouse Anti-COL4A5 Recombinant Antibody (clone 40A9)
CAT#: MOB-2325z
This product is a mouse antibody that recognizes human COL4A5. It can be used in the following applications: WB, ELISA, ICC, IHC, IP.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/77177/1975_E11_3_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced (HeLa, HHSteC, HUVEC TERT2, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000188153-COL4A5
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Specifications
- Immunogen
- Human Collagen, Type IV, Alpha 5
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human COL4A5
- Species Reactivity
- Human
- Clone
- 40A9
- Applications
- WB, ELISA, ICC, IHC, IP
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western blot, Enzyme-linked Immunosorbent Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation.
Target
- Alternative Names
- COL4A5; DA149D17.3; Collagen Type IV Alpha 5; Collagen Alpha-5(IV) Chain; ATS; CA54; ASLN; DA24A23.1; Collagen Of Basement Membrane, Alpha-5 Chain
- UniProt ID
- P29400
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Customer Review
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Good for Western Blot
The Mouse Anti-COL4A5 Recombinant Antibody worked excellently in my Western blotting experiments. The bands were sharp and clear, making data analysis much easier.
High Sensitivity
This antibody demonstrated high sensitivity in detecting low levels of COL4A5 in my samples, allowing for more intricate examination of protein expression levels.
Effective in Cell Culture
The antibody performed well in my cell culture studies, allowing me to detect cellular COL4A5 efficiently. I highly recommend it for any in vitro applications.
Q&As
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Can this antibody be used for ELISA?
A: Yes, the Mouse Anti-COL4A5 Recombinant Antibody has been verified for ELISA usage. Please refer to the procedure for optimal results.
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Can this antibody be used for frozen sections?
A: Yes, this antibody can be used on both frozen and paraffin-embedded tissue sections. Proper tissue preparation is critical for optimal results.
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Can it be used for multiplex assays?
A: Yes, the antibody is well-suited for multiplex assays due to its specificity and minimal cross-reactivity. Ensure proper controls are in place for accurate data interpretation.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "COL4A5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1951 | PE-A2/Human COL4A5 (SLWGQPAEA) MHC Tetramer | FCM | |
| MHC-LC1979 | PE-A*02:01/Human COL4A5 (SLWGQPAEA) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1594 | Anti-COL4A5 Immunohistochemistry Kit | IHC |
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