Hi-Affi™ Rabbit Anti-CREB1 Recombinant Antibody (clone TH70DS)
CAT#: MOR-4560
Recombinant Rabbit anti-CREB1 monoclonal antibody for WB, IF, ICC, FCM, ChIP, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19150/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3803/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19150/ihc_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19150/44743_A_7_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19150/44743_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19150/44743_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19150/44743_A_7_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19150/44743_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19150/44743_A_6_6.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118260-CREB1
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Specifications
- Immunogen
- Recombinant protein corresponding to amino acids 222-341 of human cAMP responsive element binding protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CREB1
- Species Reactivity
- Human
- Clone
- TH70DS
- Applications
- WB, IF, ICC, FC, ChIP
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.09% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Chromatin Immunoprecipitation (1 µl), Flow Cytometry (1:100-1:500), Immunocytochemistry (1:500-1:5000), Immunofluorescence (1:500-1:5000), Western Blot (1:500-1:5000).
Target
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Related Signaling Pathways
cAMP Signaling Pathway
MAPK Signaling Pathway
PPAR Signaling Pathway
TLR Signaling Pathway
TNF Signaling Pathway
Related Diseases
Prostate Cancer
Morphine Addiction
Cocaine Addiction
Amphetamine Addiction
Alcoholism
Cushing Syndrome
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "CREB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0781 | Hi-Affi™ Rabbit Anti-CREB1 Recombinant Antibody (clone DS781AB) | WB, IP | Rabbit IgG |
| MOR-0800 | Hi-Affi™ Rabbit Anti-CREB1 Recombinant Antibody (clone DS800AB) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
| MOR-4561 | Hi-Affi™ Rabbit Anti-CREB1 Recombinant Antibody (clone TH71DS) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1788-CN | Rabbit Anti-CREB1 Polyclonal Antibody (MRO-1788-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-QX272 | Mouse Anti-CREB1 Recombinant Antibody (clone 8D10-7D1-5B6) | WB, IHC | Mouse IgG1 |
| VS3-QX273 | Mouse Anti-CREB1 Recombinant Antibody (clone 1335CT115.203.189) | WB | Mouse IgG1, κ |
| VS3-QX274 | Mouse Anti-CREB1 Recombinant Antibody (clone 1E9-G10-C3-H6) | WB | Mouse IgG1 |
| VS3-QX275 | Mouse Anti-CREB1 Recombinant Antibody (clone 2F6-H3-E2) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY69 | AbPlus™ Anti-CREB1 Magnetic Beads (CBACN-159) | IP, Protein Purification | |
| VS-0724-YC812 | AbPlus™ Anti-CREB1 Magnetic Beads (VS-0724-YC812) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY538 | Anti-CREB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1663 | Anti-Mouse CREB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1662 | Anti-Human CREB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1664 | Anti-Rat CREB1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC244 | CytoStream™ Rabbit Anti-CREB1 Recombinant Antibody (VS13-YC244) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
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