Mouse Anti-CSF2RB Recombinant Antibody (clone BION-1) (CAT#: PABZ-029)

Figure 1 Flow cytometry analysis

Flow cytometry analysis of the staining of MoAb BION-1 (continuous line) and an isotype matched IgG, control MoAb (dotted line) to (A) COS cells transiently transfected with β_c, (B) CHO cells constitutively expressing β_c, (C) TF-1.8 cells, (D) neutrophils, (E) eosinophils and (F) monocytes.

Figure 2 Dose-dependent competition for the binding

Dose-dependent competition for the binding of ¹²⁵I-IL-5 (50 pM), ¹²⁵I-GM- CSF (50 pM) and ¹²⁵I-IL-3 (200 pM) by MoAbs BION-1, an anti-βc MoAb control, and an IgG, control MoAb to TF-1.8 cells (2×10⁶ per point). ( . . . ) represents ligand binding in the presence of 200-fold excess unlabelled ligand. Each point is the mean of triplicate determinations.

Figure 3 BION-1 blocks high-affinity binding of IL-5, GM-CSF, and IL-3 to human eosinophils.

Human eosinophils (1.8 × 10⁶) were preincubated either alone (open boxes) or in the presence of 1 μmol/L BION-1 MoAb (closed boxes) or an isotype IgG1-matched control antibody (diagonal boxes) before the addition of radiolabeled cytokine. Nonspecific binding was in each case less than 1% of total counts added. Each point is the mean of duplicate determinations and error bars represent 1 standard deviation.

Sun, Q., Jones, K., McClure, B., Cambareri, B., Zacharakis, B., Iversen, P. O., ... & Lopez, A. F. (1999). Simultaneous antagonism of interleukin-5, granulocyte-macrophage colony-stimulating factor, and interleukin-3 stimulation of human eosinophils by targetting the common cytokine binding site of their receptors. Blood, 94(6), 1943-1951.

Figure 4 The binding of BION-1 to TF1.8 cells is inhibited by IL-3 but not by TNFα.

TF1.8 cells (2 × 10⁶) were incubated with a range of concentrations of IL-3 (closed circles) or TNFα (open circles) in the presence of ¹²⁵I-labeled MoAb BION-1 (1 nmol/L). Each point is the mean of duplicate determinations and error bars represent 1 standard deviation.

Sun, Q., Jones, K., McClure, B., Cambareri, B., Zacharakis, B., Iversen, P. O., ... & Lopez, A. F. (1999). Simultaneous antagonism of interleukin-5, granulocyte-macrophage colony-stimulating factor, and interleukin-3 stimulation of human eosinophils by targetting the common cytokine binding site of their receptors. Blood, 94(6), 1943-1951.

Figure 5 Inhibition of IL-3–induced α and β chain dimerization and phosphorylation by MoAb BION-1.

(A) Immunoprecipitations using anti–IL-3Rα MoAb, 9F5, or anti-bc MoAb, 8E4, from ¹²⁵I-surfacelabeled MO7e cells preincubated with MoAbs BION-1, control anti-βc MoAb 1C1, or medium alone (-), before being stimulated (+) or not (-) with IL-3. The radiolabeled proteins were spearated by SDS-PAGE and visualized by PhosphorImaging. The position and molecular weight of marker proteins are shown. (B) Immunoprecipitated proteins were probed using antiphosphotyrosine MoAb 3-365-10.

Sun, Q., Jones, K., McClure, B., Cambareri, B., Zacharakis, B., Iversen, P. O., ... & Lopez, A. F. (1999). Simultaneous antagonism of interleukin-5, granulocyte-macrophage colony-stimulating factor, and interleukin-3 stimulation of human eosinophils by targetting the common cytokine binding site of their receptors. Blood, 94(6), 1943-1951.