Anti-Human CXCR2 Therapeutic Antibody (163E3) (CAT#: TAB-003MZ-VHH)

This antibody specifically binding to chemokine receptor CXCR2 may be used for treatment of diseases involving aberrant functioning of CXCR2.

  • Published Data
  • Datasheet
  • MSDS
  • COA
Activ

Figure 1 GRO - a concentration response curves in the presence of increasing concentrations of nanobody

The response curves obtained when the ability of 163E3 to block [ SS ] GTPyS release from CHO-CXCR2 membranes stimulated with agonist Gro - a at is measured at increasing concentrations of Nanobody

Inhib

Figure 2 Effect of increasing CXCL1 concentration on anti-CXCR2 nanobody inhibition of CXCL1-stimulated [35S]GTPγS binding assay

163E3 is able to fully inhibit [³⁵S]GTPγS binding at all of the CXCL1 concentrations tested.

Bradley, M. E., Dombrecht, B., Manini, J., Willis, J., Vlerick, D., De Taeye, S., ... & Laeremans, T. (2015). Potent and efficacious inhibition of CXCR2 signaling by biparatopic nanobodies combining two distinct modes of action. Molecular pharmacology, 87(2), 251-262.

Inhib

Figure 3 Schild experiments performed using CXCL1-stimulated [³⁵S]GTPγS binding assay

CHO-CXCR2 cell membranes, in the presence of a range of concentrations of (A) 127D1 and (B) 163E3 anti-CXCR2 nanobodies.

Bradley, M. E., Dombrecht, B., Manini, J., Willis, J., Vlerick, D., De Taeye, S., ... & Laeremans, T. (2015). Potent and efficacious inhibition of CXCR2 signaling by biparatopic nanobodies combining two distinct modes of action. Molecular pharmacology, 87(2), 251-262.

Inhib

Figure 4 Inhibition of FLAG-tagged nanobody binding to CHO-CXCR2 cells by monovalent nanobodies

Inhibition of FLAG-tagged nanobody binding to CHO-CXCR2 cells by monovalent nanobodies (A) using FLAG-tagged 163E3 and (B) using FLAG-tagged 127D1.

Bradley, M. E., Dombrecht, B., Manini, J., Willis, J., Vlerick, D., De Taeye, S., ... & Laeremans, T. (2015). Potent and efficacious inhibition of CXCR2 signaling by biparatopic nanobodies combining two distinct modes of action. Molecular pharmacology, 87(2), 251-262.

Inhib

Figure 5 Effect of generating biparatopic nanobody construct on potency in CXCL1-stimulated [35S]GTPγS binding assay

Effect of generating biparatopic nanobody construct on potency in CXCL1-stimulated [³⁵S]GTPγS binding assay using CHO-CXCR2 cell membranes.

Bradley, M. E., Dombrecht, B., Manini, J., Willis, J., Vlerick, D., De Taeye, S., ... & Laeremans, T. (2015). Potent and efficacious inhibition of CXCR2 signaling by biparatopic nanobodies combining two distinct modes of action. Molecular pharmacology, 87(2), 251-262.

Inhib

Figure 6 Inhibition of basal [³⁵S]GTPγS binding to CHO-CXCR2 cell membranes

Inhibition of basal [³⁵S]GTPγS binding to CHO-CXCR2 cell membranes using monovalent and biparatopic nanobodies.

Bradley, M. E., Dombrecht, B., Manini, J., Willis, J., Vlerick, D., De Taeye, S., ... & Laeremans, T. (2015). Potent and efficacious inhibition of CXCR2 signaling by biparatopic nanobodies combining two distinct modes of action. Molecular pharmacology, 87(2), 251-262.


Specifications

  • Host Species
  • Human
  • Type
  • Single domain antibody
  • Specificity
  • Human
  • Clone
  • 163E3
  • Applications
  • FC, Inhib, Activ
  • Related Disease
  • Chronic obstructive pulmonary disease (COPD)

Applications

  • Application Notes
  • The antibody was validated for Inhibition, Activation. For details, refer to Published Data.

Target

  • Alternative Names
  • CXCR2; chemokine (C-X-C motif) receptor 2; CD182; IL8R2; IL8RA; IL8RB; CMKAR2; CDw128b; C-X-C chemokine receptor type 2; CXC-R2; CXCR-2; IL-8R B; GRO/MGSA receptor; IL-8 receptor type 2; interleukin 8 receptor B; chemokine (CXC) receptor 2; interleukin 8 receptor, beta; interleukin 8 receptor type 2; interleukin-8 receptor type B; CXCR2 gene for IL8 receptor type B; high affinity interleukin-8 receptor B

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "CXCR2"

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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