Mouse Anti-Carbohydrate Recombinant Antibody (clone SE155-4) (CAT#: PABZ-018)
Recombinant Mouse Antibody (SE155-4) is capable of binding to Carbohydrate. Se155-4 precipitated the polysaccharide antigen from serogroup B Salmonella, indicating that it bound epitopes distributed along the polysaccharide chain.
Specific Inquiry
Figure 1 Scatchard plots for the determination of affinities from equilibrium binding.
A, monomeric wild-type scFv. B, dimeric B5-1. C, Se155-4 IgG.
MacKenzie, C. R., Hirama, T., Deng, S. J., Bundle, D. R., Narang, S. A., & Young, N. M. (1996). Analysis by surface plasmon resonance of the influence of valence on the ligand binding affinity and kinetics of an anti-carbohydrate antibody. Journal of Biological Chemistry, 271(3), 1527-1533.
Figure 2 Relative response versus time for the binding to immobilized BSA-O-polysaccharide of wild-type Se155-4 scFv containing trace amounts of dimer
(A), monomeric wild-type scFv obtained by size-exclusion HPLC (B), and mutant B5-1, which exists almost entirely in the dimeric form (C). The concentrations are (from bottom to top) 1, 1.5, 2, 3, and 5 μM in A; 0.2, 0.4, 0.8, 1.2, and 1.6 μM in B; and 20, 40, 80, 120, and 200 nM in C.
MacKenzie, C. R., Hirama, T., Deng, S. J., Bundle, D. R., Narang, S. A., & Young, N. M. (1996). Analysis by surface plasmon resonance of the influence of valence on the ligand binding affinity and kinetics of an anti-carbohydrate antibody. Journal of Biological Chemistry, 271(3), 1527-1533.
Figure 3 Specificity of Se155-4 for LPS of different serogroups and synthetic glycoconjugates in EIA.
Bundle, D. R., Eichler, E., Gidney, M. A. J., Meldal, M., Ragauskas, A., Sigurskjold, B. W., ... & Young, N. M. (1994). Molecular recognition of a Salmonella trisaccharide epitope by monoclonal antibody Se155-4. Biochemistry, 33(17), 5172-5182.
Figure 4 ELISA assays of wild-type and Cys94 modified forms of Se155-4.
IgG (filled square); unmodified scFv S94C (open square); S94C modified with iodoacetamide (filled triangle); scFv S94C modified with IANDB (filled inverted triangle); scFv S94C modified with Fe-BABE (open circle).
Young, N. M., Watson, D. C., Cunningham, A. M., & MacKenzie, C. R. (2014). The intrinsic cysteine and histidine residues of the anti-Salmonella antibody Se155-4: a model for the introduction of new functions into antibody-binding sites. Protein Engineering, Design & Selection, 27(10), 383-390.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1, λ
- Specificity
- Carbohydrate
- Clone
- SE155-4
- Applications
- WB, IF, FuncS
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Carbohydrate
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone SE155-4"
- PFBC-098
- Recombinant Mouse Anti-P. salmonella Carbohydrate (Trisaccharide) Antibody Fab Fragment (SE155-4)
See other products for "carbohydrate"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-153 | Recombinant Anti-carbohydrate Antibody | ELISA, WB, IF, FuncS | IgG |
| MHH-153 | Recombinant Human Anti-carbohydrate Antibody | ELISA, IF, FuncS | IgG |
| PABC-018 | Mouse Anti-Carbohydrate Recombinant Antibody (clone BR96) | WB, ELISA, IHC, Inhib, FuncS | Mouse IgG3, κ |
| PABC-098 | Recombinant Mouse Anti-P. salmonella Carbohydrate (Trisaccharide) Antibody (SE155-4) | WB, FuncS | IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-153-F(E) | Recombinant Anti-carbohydrate Antibody Fab Fragment | IF, FC, FuncS | Fab |
| MHH-153-F(E) | Recombinant Human Anti-carbohydrate Antibody Fab Fragment | ELISA, WB, FuncS | Fab |
| PFBC-018 | Mouse Anti-Carbohydrate Recombinant Antibody (clone BR96); Fab Fragment | WB, FuncS | Mouse IgG3, κ |
| PFBZ-018 | Mouse Anti-Carbohydrate Recombinant Antibody (clone SE155-4); Fab Fragment | WB, IF, FuncS | Mouse IgG1, λ |
| PFBC-098 | Recombinant Mouse Anti-P. salmonella Carbohydrate (Trisaccharide) Antibody Fab Fragment (SE155-4) | WB, FuncS | IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-153-S(P) | Recombinant Anti-carbohydrate Antibody scFv Fragment | WB, FC, FuncS | scFv |
| MHH-153-S(P) | Recombinant Human Anti-carbohydrate Antibody scFv Fragment | WB, IP, FuncS | scFv |
| PSBC-018 | Mouse Anti-Carbohydrate Recombinant Antibody (clone BR96); scFv Fragment | WB, FuncS | Mouse scFv |
| PSBZ-018 | Mouse Anti-Carbohydrate Recombinant Antibody (clone SE155-4); scFv Fragment | WB, IF, FuncS | Mouse scFv |
| PSBC-098 | Recombinant Mouse Anti-P. salmonella Carbohydrate (Trisaccharide) Antibody scFv Fragment (SE155-4) | WB, FuncS | scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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