Rat Anti-Clec4e Recombinant Antibody (NEUT-470CQ)
CAT#: NEUT-470CQ
This antibody is a rat monoclonal antibody that binds specifically to mMincle, and it can neutralize the bioactivity of mMincle.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4935/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4935/13398_B_7_5.jpg
Normal Tissue
Figure 3 Cerebellum
Cells in granular layer Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Cells in molecular layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Purkinje cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4935/13398_B_7_8.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4935/13398_A_7_5.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (NB-4, RPTEC TERT1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166523-CLEC4E
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Specifications
- Host Species
- Rat
- Type
- Rat IgG2b
- Specificity
- Mouse Clec4e
- Species Reactivity
- Mouse
- Applications
- Neut, FC
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Excellent Flow Cytometry Compatibility
I've included this antibody in my flow cytometry protocol, and it's been effective. The antibody binds selectively to Clec4e, allowing for accurate cell population characterisation.
Superior Sensitivity
The sensitivity of the Rat Anti-Clec4e Recombinant Antibody (clone 6G5) is noteworthy. I was able to detect low quantities of Clec4e in my samples, which is critical when investigating proteins with low expression.
Ideal for Detailed Studies
This antibody is appropriate for research that require a close look at Clec4e expression levels. Its performance in a wide range of assays has tremendously aided my research efforts.
Q&As
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How does antibody concentration impact immunoassay results?
A: Optimal antibody concentration enhances target detection while minimizing background signals; perform titrations to establish the best concentration.
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What are chimeric antibodies?
A: Chimeric antibodies combine variable regions of one species with constant regions of another, enhancing compatibility for certain applications.
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Can detergents like Triton X-100 affect antibody binding?
A: Membrane protein solubilization, and consequently antibody binding, can be influenced by detergents. Optimize type and concentration for the desired assay.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "CLEC4E"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3220z | Mouse Anti-CLEC4E Recombinant Antibody (clone 27D8) | WB, ICC, IF, IHC, FC, ELISA | Mouse IgG2b |
| MOB-1110MZ | Recombinant Mouse Anti-Human CLEC4E Antibody (clone BU27F4) | ELISA, IHC-P, WB | Mouse antibody |
| FN-114CQ | Rat Anti-Clec4e Recombinant Antibody (FN-114CQ) | FuncS | Rat IgG1, κ |
| FN-116CQ | Rat Anti-CLEC4E Recombinant Antibody (FN-116CQ) | FC | Rat IgG2b, κ |
| FN-118CQ | Mouse Anti-CLEC4E Recombinant Antibody (FN-118CQ) | FuncS | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-469CQ | Mouse Anti-CLEC4E Recombinant Antibody (NEUT-469CQ) | Neut, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1500 | Anti-CLEC4E Immunohistochemistry Kit | IHC |
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