Recombinant Mouse Anti-DENV E-DIII Antibody (1A1D-2)
CAT#: PABC-031
Recombinant Mouse Antibody (1A1D-2) is capable of binding to DENV E-DIII, expressed in Chinese Hamster Ovary cells (CHO). The monoclonal antibody 1A1D-2 has been shown to strongly neutralize dengue virus serotypes 1, 2 and 3, primarily by inhibiting attachment to host cells.
Published Data
Tested Data
SPR
Figure 2 Mouse Anti-DENV E-DIII Antibody (1A1D-2) in SPR.
SPR sensorgrams for Fab of 1A1D-2 WT and mutants to DENV1-4. In the SPR experiments, recombinant DENV EDIII was covalently immobilized on the CM5 chip (Cytiva). A series of 1A1D-2 Fab (WT or mutants) in two-fold dilutions were flowed through the chip for two minutes and allowed to dissociated for three minutes. Colored lines represent experiment data at different analyte concentrations, and the black lines are fitting curve. Each experiment was independently repeated for three times. For DENV4, the highest concentration used in this experiment reached 3200 nM for recombinant EDIII proteins. The color keys were provided below the graphs.
Wei, H., Tan, J., Zhou, B., Guan, X., Zhong, Q., & Wang, J. (2022). Charged Residue Implantation Improves the Affinity of a Cross-Reactive Dengue Virus Antibody. International journal of molecular sciences, 23(8), 4197.
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SDS-PAGE
Figure 1 Recombinant Mouse Anti-DENV E-DIII Antibody (PABC-031) in SDS-PAGE
SDS-PAGE analysis of PABC-031 in non-reduced and β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 2 Recombinant Mouse Anti-DENV E-DIII Antibody (PABC-031) in HPLC
The purity of PABC-031 was greater than 95% as determined by SEC-HPLC.
WB
Figure 3 Recombinant Mouse Anti-DENV E-DIII Antibody (PABC-031) in WB
Western blot analysis of PABC-031 was performed by loading Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E / Envelope Protein (ECD, His Tag).
PABC-031 incubation concentration: 2ng/μL.
The secondary antibody: HRP-Goat-Anti-Mouse IgG (H+L).
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 2: Reducing Antigen (0.6 μg)
ELISA
Figure 4 Recombinant Mouse Anti-DENV E-DIII Antibody (PABC-031) in ELISA
ELISA analysis of PABC-031 was performed by coating with Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E / Envelope Protein (ECD, His Tag).
The secondary antibody: HRP-Goat-Anti-Mouse IgG (H+L).
SDS-PAGE
Figure 5 Recombinant Mouse Anti-DENV E-DIII Antibody (PABC-031) in SDS-PAGE
SDS-PAGE analysis of PABC-031 in non-reduced and β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 6 Recombinant Mouse Anti-DENV E-DIII Antibody (PABC-031) in SEC-HPLC
The purity of PABC-031 was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
WB
Figure 7 Recombinant Mouse Anti-DENV E-DIII Antibody (PABC-031) in WB
Western blot analysis of PABC-031 was performed by loading Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E / Envelope Protein (ECD, His Tag).
PABC-031 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat-Anti-Mouse IgG (H+L).
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 2: Reducing Antigen (0.6 μg)
ELISA
Figure 8 Recombinant Mouse Anti-DENV E-DIII Antibody (PABC-031) in ELISA
ELISA analysis of PABC-031 was performed by coating with Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E / Envelope Protein (ECD, His Tag).
The secondary antibody: HRP-Goat-Anti-Mouse IgG (H+L).
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Specifications
- Immunogen
- DENV Dengue virus E-DIII
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native DENV E-DIII
- Species Reactivity
- DENV
- Clone
- 1A1D-2
- Applications
- Can be useful in applications such as: Western Blot, Enzyme-linked Immunosorbent Assay
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
- Alternative Names
- Dengue virus; DENV; dengue virus E-DIII; DENV E-DIII
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(Creative Biolabs Cat# PABC-031, RRID: AB_3714142)
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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COA
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-275 | Recombinant Anti-DENV E-DIII Antibody | ELISA, WB, FC, FuncS | IgG |
| MHH-275 | Recombinant Human Anti-DENV E-DIII Antibody | ELISA, WB, FC, FuncS | IgG |
| PABL-437 | Recombinant Mouse Anti-DENV EDIII Antibody (2H12) | ELISA, Neut | IgG |
| MRO-1612CQ | Recombinant Human Anti-DENV E-DIII Antibody (DV10) | ELISA, Neut | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-275-F(E) | Recombinant Anti-DENV E-DIII Antibody Fab Fragment | ELISA, WB, FuncS | Fab |
| MHH-275-F(E) | Recombinant Human Anti-DENV E-DIII Antibody Fab Fragment | ELISA, WB, Neut, FuncS | Fab |
| PFBC-031 | Recombinant Mouse Anti-DENV E-DIII Antibody Fab Fragment (1A1D-2) | WB, ELISA | IgG |
| PFBL-434 | Recombinant Mouse Anti-DENV EDIII Antibody Fab Fragment (2H12) | ELISA, Neut | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-275-S(P) | Recombinant Anti-DENV E-DIII Antibody scFv Fragment | RIA, ELISA, FuncS | scFv |
| MHH-275-S(P) | Recombinant Human Anti-DENV E-DIII Antibody scFv Fragment | Neut, IF, FuncS | scFv |
| PSBC-031 | Recombinant Mouse Anti-DENV E-DIII Antibody scFv Fragment (1A1D-2) | WB, ELISA | scFv |
| PSBL-434 | Recombinant Mouse Anti-DENV EDIII Antibody scFv Fragment (2H12) | ELISA, Neut | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-044 | Recombinant Mouse Anti-DENV Antibody (3E31) | WB, ELISA, FuncS, Neut, Inhibt | IgG |
| PABX-045 | Recombinant Mouse Anti-DENV Antibody (PABX-045) | WB, ELISA, FuncS, IF | IgG |
| PABX-054 | Recombinant Mouse Anti-DENV Antibody (E111) | WB, ELISA, FuncS, SPR, Neut | IgG |
| PABX-044-S (P) | Recombinant Mouse Anti-DENV Antibody scFv Fragment (3E31) | WB, ELISA, FuncS | scFv |
| PABX-044-F (E) | Recombinant Mouse Anti-DENV Antibody Fab Fragment (3E31) | WB, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
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| PABX-054-S (P) | Recombinant Mouse Anti-DENV Antibody scFv Fragment (E111) | WB, ELISA, FuncS | scFv |
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