Recombinant Mouse Anti-DENV precursor peptide Antibody (2H2)

(A) Survival of DENV-infected AG129 mice treated on days −1 and 1 with 15 μg of anti-DENV antibody (black squares, n = 7–12) or an isotype control (white squares, n = 12–20). Various anti-DENV antibodies were used, see figure and text for details. As the same isotype control was used for both IgG2a antibodies, the same isotype control curve is depicted in the left and middle panels. (C) Survival of AG129 mice infected intradermally (i.d.) with 5 × 10⁸ GE S221 and treated with anti-DENV antibodies (black squares, n = 7) or isotype control (white squares, n = 7). IgG2a (15 μg) against DENV prM/M was administered i.p. on days −1 and 1. (D) Survival of AG129 mice infected with 5 × 10⁷ or 5 × 10⁶ GE S221 i.v. and treated with anti-DENV antibodies (black squares, n = 4) or isotype control (white squares, n = 4). Antibody was administered as in (C).

Zellweger, R.M. Prestwood, T.R. Shresta, S. (2010). Enhanced Infection of Liver Sinusoidal Endothelial Cells in a Mouse Model of Antibody-Induced Severe Dengue Disease. Cell Host & Microbe 7, 128–139.

Cells were washed after 1.5 h and incubated for ∼24 h. Intracellular staining of DENV antigen was performed with a DyLight-649-conjugated antibody against prM and infection was determined by flow cytometry. Experiment is representative of three with similar results.

Chan, Y.K. Huang, I.H. Farzan, M. (2012). IFITM Proteins Restrict Antibody-Dependent Enhancement of Dengue Virus Infection. PLOS ONE.

(A) AG129 mice were immunized with 1×106 IU DENV2 E85-VRP either i.f. (black circles) or i.p. (black triangles) 14 and 5 days prior to challenge with 5×108 GE DENV. Two groups of mice were not immunized prior to infection with DENV, one of which was infected in the presence of anti-DENV antibody 2H2, resulting in antibody-mediated enhancement of disease (black squares, ADE group) and the other one was infected in the presence of antibody C1.18 which is an isotype control of irrelevant specificity (white squares, baseline group). Viral RNA levels were measured in the liver 3 days after challenge. (C) One day prior to challenge, serum DENV-specific IgG were measured by ELISA on DENV-coated plates.

Zellweger, R. M., Miller, R., Eddy, W. E., White, L. J., Johnston, R. E., & Shresta, S. (2013). Role of humoral versus cellular responses induced by a protective dengue vaccine candidate. PLoS pathogens, 9(10), e1003723.