Recombinant Mouse Anti-DNA Antibody (ED10) (CAT#: PABW-032)

Figure 1 Northern and Western blot analyses of chDab1 expression.

(A) Northern blots were prepared using poly(A) þ RNA (2 mg/ lane) extracted from the retina (ED5, ED10, ED16), brain (ED5, ED10, ED16), heart (ED5, ED15), liver (ED5, ED10, ED16), kidney (ED7, ED16) and gut (ED16). The filter was sequentially hybridized with 32P-labeled: (i) 532 bp chDab1 cDNA and (ii) actin cDNA. The extra bands obtained with the actin probe in the heart and gut represent tissue-specific actin mRNAs. We consistently find actin RNA to be low in the liver, especially at later developmental stages.
(B) Western blots were prepared using ED4, ED7 and ED16 total chick retina extracts (50 mg protein/lane). The filter was sequentially incubated with rabbit anti-Dab1 antibody (1:5000) and goat anti-actin antibody (1:500). Molecular mass standards (in kDa) areM indicated on the left.

Katyal, S., & Godbout, R. (2004). Alternative splicing modulates Disabled‐1 (Dab1) function in the developing chick retina. The EMBO journal, 23(8), 1878-1888.

Figure 2 RT–PCR analysis of chDab1 deletion and insertion regions.

(A) cDNAs synthesized from poly(A) þ RNA from the retina (ED5, ED7, ED10, ED16) and brain (ED3.5, ED5, ED16) were amplified using P1 and P2 primers for deletion analysis and P3 and P4 primers for insertion analysis. Sizes of amplified bands are indicated. (B) Banding patterns obtained by RT–PCR analysis of ED5, ED10 and ED16 retina using P1 and P4 primers. The lower band is 382 bp and the higher band is 430 bp.

Katyal, S., & Godbout, R. (2004). Alternative splicing modulates Disabled‐1 (Dab1) function in the developing chick retina. The EMBO journal, 23(8), 1878-1888.