Human Anti-DPP4 Recombinant Antibody (HPAB-0215-YC) (CAT#: HPAB-0215-YC)

This product is a recombinant humanized antibody that recognizes DPP4. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography. The anti-proliferative effect of specified recombinant human MAb was analyzed in Jurkat CD26+ cells and the most inhibitory concentration was at 5 μg/mL. The antibody has an a discontinuous or conformational epitope. It had high reactivity against peptides: YSLRWISDHEYLY, YVKQWRHSYTASY, TWSPVGHKLAYVW, EEEVFSAYSALWW and RISLQWLRRIQNY.


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WB

Figure 1 Anti-DPP4 Recombinant Antibody (HPAB-0215-YC) in WB

Figure 1 Anti-DPP4 Recombinant Antibody (HPAB-0215-YC) in WB

Western blot analysis of HPAB-0215-YC (2 μg/mL) was performed with Recombinant Human DPP4/CD26 Protein (His Tag). The HRP-Anti-Human IgG (H+L) (1: 6000) was used as a secondary antibody.

Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg

DB

Figure 2 Anti-DPP4 Recombinant Antibody (HPAB-0215-YC) in DB

Figure 2 Anti-DPP4 Recombinant Antibody (HPAB-0215-YC) in DB

Dot Blot analysis of HPAB-0215-YC (2 μg/mL) was performed by coating with Recombinant Human DPP4/CD26 Protein (His Tag). The HRP-Anti-Human IgG (H+L) (1: 6000) was used as a secondary antibody.

ELISA

Figure 3 Anti-DPP4 Recombinant Antibody (HPAB-0215-YC) in ELISA

Figure 3 Anti-DPP4 Recombinant Antibody (HPAB-0215-YC) in ELISA

ELISA analysis of HPAB-0215-YC was performed by coating with Recombinant Human DPP4/CD26 Protein (His Tag). Then blocked with BSA, and incubated with HPAB-0215-YC. The HRP-conjugated goat Anti-Human IgG was used as a secondary antibody (1:5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.


Specifications

  • Host Species
  • Human
  • Derivation
  • Humanized
  • Type
  • Humanized IgG1
  • Specificity
  • Human DPP4
  • Species Reactivity
  • Human
  • Applications
  • ELISA, Inhib, FuncS
  • Related Disease
  • Diseases associated with expression of CD26

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • Dipeptidyl-Peptidase 4; Adenosine Deaminase Complexing Protein 2; Dipeptidylpeptidase IV (CD26, Adenosine Deaminase Complexing Protein 2); T-Cell Activation Antigen CD26; Dipeptidyl Peptidase IV; EC 3.4.14.5; DPP IV; ADCP-2; ADCP2; ADABP; TP103; CD26; Dipeptidylpeptidase 4; CD26 Antigen; DPPIV

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "DPP4"

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1041 Hi-Affi™ Rabbit Anti-DPP4 Recombinant Antibody (clone DS1041AB) WB Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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