Recombinant Anti-E. coli dhfr VHH Single Domain Antibody (PNBL-075)
CAT#: PNBL-075
Anti-E. coli dhfr VHH Single Domain Antibody is a recombinant protein produced in E. coli.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51465/766_F2_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51465/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/ihc_selected.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/73545_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/73545_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/73545_A_5_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000228716-DHFR
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Specifications
- Host Species
- Llama
- Derivation
- Llama
- Type
- Llama VHH
- Species Reactivity
- E. coli
- Clone
- PNBL-075
- Applications
- Can be useful in applications such as: Western Blot, Functional Study
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
- Alternative Names
- dihydrofolate reductase; dhfr
REVIEWS AND Q&AS
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Customer Review
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Excellent Bind Affinity
The Recombinant Anti-E. coli dhfr VHH Single Domain Antibody (PNBL-075) demonstrates excellent binding affinity in all my WB assays. This has significantly improved the sensitivity of my detection methods.
Works Well in Western Blots
I've been using this antibody for Western Blotting with great success. The bands are sharp and clear, and it specifically targets the DHFR protein without any cross-reactivity.
Optimal for Research
This antibody is optimal for E. coli DHFR research. It's easy to use and provides high specificity and sensitivity in various assay formats.
Q&As
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Can this antibody be used for Western Blotting?
A: Indeed, Western Blotting can be performed with the Recombinant Anti-E. coli dhfr VHH Single Domain Antibody (PNBL-075). The ideal dilution ratios might vary from 1:500 to 1:5,000 based on the circumstances and particular use.
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Are there any known cross-reactivities?
A: Recombinant Anti-E. coli dhfr VHH Single Domain Antibody (PNBL-075) does not exhibit considerable cross-reactivity with non-target proteins and is highly specific for the E. coli DHFR protein.
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What host organism was used to produce this antibody?
A: The camelid (Camelidae) system, which was created especially to make single-domain antibodies with excellent specificity and affinity, was used to produce this recombinant antibody.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "DHFR"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1812z | Mouse Anti-DHFR Recombinant Antibody (clone 30C7) | WB, ICC, IHC | Mouse IgG2b |
| MOB-0052CT | Recombinant Mouse anti-Human DHFR Monoclonal antibody (4B22) | IHC-P, WB | |
| ZG-0636J | Rabbit Anti-DHFR Recombinant Antibody (clone 9B2) | ELISA, WB, IHC, IP | Rabbit IgG |
| VS3-XY3331 | Rabbit Anti-DHFR Recombinant Antibody (clone R05-2C7) | WB, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-013 | Recombinant Anti-E. coli DHFR VHH Single Domain Antibody (PNBL-013) | FACS, ELISA, FuncS | Llama VHH |
| PNBL-014 | Recombinant Anti-E. coli DHFR VHH Single Domain Antibody (PNBL-014) | WB, FuncS | Llama VHH |
| PNBL-074 | Recombinant Anti-E. coli DHFR VHH Single Domain Antibody (PNBL-074) | NMR, ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0976 | Hi-Affi™ Rabbit Anti-DHFR Recombinant Antibody (clone DS976AB) | ICC, IF, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY163 | Mouse Anti-NHP DHFR Recombinant Antibody (clone OTI6G7) | WB, IHC, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1984 | Anti-DHFR Immunohistochemistry Kit | IHC | |
| VS-0525-XY1985 | Anti-Mouse DHFR Immunohistochemistry Kit | IHC | |
| VS-0525-XY1986 | Anti-Canine DHFR Immunohistochemistry Kit | IHC |
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