Mouse Anti-Estradiol Recombinant Antibody (clone 10G6D6) (CAT#: PABW-037)

Recombinant Mouse Antibody (10G6D6) is capable of binding to E2 (17β-estradiol), expressed in Chinese Hamster Ovary cells (CHO).


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Figure 1 Ability of the 17E12E5 scFV mutants to bind to estradiol as determined from ELISA.

Figure 1 Ability of the 17E12E5 scFV mutants to bind to estradiol as determined from ELISA.

Histograms a and b correspond to light and heavy variable domains mutants respectivity. The continuous line reflects the limit of functionality of the mutated residue.

Bettsworth, F., Monnet, C., Watelet, B., Battail‐Poirot, N., Gilquin, B., Jolivet, M., ... & Ducancel, F. (2001). Functional characterization of two anti‐estradiol antibodies as deduced from modelling and site‐directed mutagenesis experiments. Journal of Molecular Recognition, 14(2), 99-109.

Figure 2 WB of crude periplasmic extracts of scFV (lane b) fused to the bacterial alkaline phosphatase.

Figure 2 WB of crude periplasmic extracts of scFV (lane b) fused to the bacterial alkaline phosphatase.

Proteins were revealed using a murine monoclonal antibody anti-PhoA and a murine monoclonal antibody anti-PhoA and a polyclonal antibody anti-mouse IgG labeled with horseradish peroxydase.

Bettsworth, F., Monnet, C., Watelet, B., Battail‐Poirot, N., Gilquin, B., Jolivet, M., ... & Ducancel, F. (2001). Functional characterization of two anti‐estradiol antibodies as deduced from modelling and site‐directed mutagenesis experiments. Journal of Molecular Recognition, 14(2), 99-109.


Specifications

  • Immunogen
  • 17β-estradiol
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Clone
  • 10G6D6
  • Applications
  • ELISA, Block

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The Estradiol antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Western Blot.

Target

  • Alternative Names
  • Estradiol; 17β-estradiol; 17beta-estradiol

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone 10G6D6"

See other products for "Estradiol"

Mouse Antibody

Human Antibody

CAT Product Name Application Type
PABX-055-S (P) Recombinant Mouse Anti-Estradiol Antibody scFv Fragment (57-2) WB, ELISA, FuncS scFv

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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