Recombinant Human Anti-EBOV GP Antibody (KZ52 ) (CAT#: PABL-458)
Recombinant Human Antibody (KZ52 ) is capable of binding to EBOV GP, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-EBOV GP mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-EBOV GP proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
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Figure 1 Reactivity of human anti-Ebola virus Fab KZ52 with a γ-irradiated whole-virion preparation (open squares) and with irradiated crude infected-cell supernatants (solid circles) determined by ELISA.
Ovalbumin (4 μg/ml) is included as a control antigen (open diamonds). OD405, optical density at 405 nm.
Maruyama, T., Rodriguez, L. L., Jahrling, P. B., Sanchez, A., Khan, A. S., Nichol, S. T., ... & Burton, D. R. (1999). Ebola virus can be effectively neutralized by antibody produced in natural human infection. Journal of virology, 73(7), 6024-6030.
Figure 2 Reactivity of anti-Ebola virus Fabs with live and fixed Ebola virus-infected Vero E6 cells shown by immunofluorescence.
Staining is shown for Fab KZ52 on live (A) and fixed (D) Ebola virus-infected cells, for Fab LZ51 on live (B) and fixed (E) Ebola virus-infected cells, and for ELZ510 on live (C) and fixed (F) Ebola virus-infected cells.
Maruyama, T., Rodriguez, L. L., Jahrling, P. B., Sanchez, A., Khan, A. S., Nichol, S. T., ... & Burton, D. R. (1999). Ebola virus can be effectively neutralized by antibody produced in natural human infection. Journal of virology, 73(7), 6024-6030.
Figure 3 Longer-exposure autoradiograms obtained to reveal the immunoprecipitation of Fab KZ52 to GP2.
Exposure of autoradiograms for 2.5 months (compared to 1 week for panel B) reveals immunoprecipitation of a band corresponding to GP2 by Fab KZ52 from Ebola virus-infected cell supernatants (lanes 1) and from crude infected-cell supernatants (lanes 2). Lanes 3 is uninfected-cell supernatant controls.
Maruyama, T., Rodriguez, L. L., Jahrling, P. B., Sanchez, A., Khan, A. S., Nichol, S. T., ... & Burton, D. R. (1999). Ebola virus can be effectively neutralized by antibody produced in natural human infection. Journal of virology, 73(7), 6024-6030.
Specifications
- Immunogen
- EBOV spike glycoprotein
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native EBOV GP
- Species Reactivity
- EBOV
- Clone
- KZ52
- Applications
- Neut Assay-depended
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- Assay-depended
Target
- Alternative Names
- Ebola virus; EBOV; Ebola virus glycoprotein; EBOV glycoprotein
- Gene ID
- 911829
- UniProt ID
- Q05320
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-298 | Recombinant Anti-EBOV GP Antibody | WB, Neut, FuncS | IgG |
| MHH-298 | Recombinant Human Anti-EBOV GP Antibody | IF, FC, Biosensors, FuncS | IgG |
| HPAB-N0106-YC | Macaque Anti-EBOV GP Recombinant Antibody (HPAB-N0106-YC) | ELISA, FC, Neut | Macaque IgG |
| HPAB-0411-FY | Human Anti-EBOV GP Recombinant Antibody (HPAB-0411-FY) | ELISA, FC, FuncS, Neut | Human IgG |
| PABJ-0009 | Human Anti-EBOV GP Recombinant Antibody (PABJ-0009) | ELISA, Neut | Human IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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