Hi-Affi™ Rabbit Anti-EPAS1 Recombinant Antibody (clone DS1161AB)
CAT#: MOR-1161
Recombinant Rabbit anti-EPAS1 monoclonal antibody for WB, FC, ICC/IF, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31200/1391_B7_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & actin filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69697/1382_A10_1_selected.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (BEWO, BJ hTERT+, HHSteC, RT4, TIME, U-138 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000116016-EPAS1
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Specifications
- Immunogen
- Synthetic peptide within Human HIF-2-alpha aa 50-150 (Cysteine residue).
- Host Species
- Rabbit
- Derivation
- Phage display library
- Type
- Rabbit IgG
- Specificity
- Human EPAS1
- Species Reactivity
- Mouse, Rat, Human
- Clone
- DS1161AB
- Applications
- WB, FC, ICC, IF
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Endothelial PAS Domain Protein 1; Class E Basic Helix-Loop-Helix Protein 73; Basic-Helix-Loop-Helix-PAS Protein MOP2; Hypoxia-Inducible Factor 2-Alpha; PAS Domain-Containing Protein 2; Member Of PAS Protein 2; HIF-1-Alpha-Like Factor; HIF-2-Alpha; HIF2-Alpha; BHLHe73; EPAS-1; HIF2A; PASD2; MOP2; HLF; Hypoxia-Inducible Factor 2 Alpha; HIF-1 Alpha-Like Factor; HIF-1alpha-Like Factor; ECYT4
- Gene ID
- 2034
- UniProt ID
- Q99814
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "EPAS1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4055z | Mouse Anti-EPAS1 Recombinant Antibody (clone 13F1) | WB, IP, IF, IHC, ELISA | Mouse IgG1, κ |
| MOB-0918CT | Recombinant Mouse anti-Human EPAS1 Monoclonal antibody (fq200c) | ELISA, IHC-Fr, IHC-P, WB | |
| MRO-0737-CN | Rabbit Anti-EPAS1 Recombinant Antibody (clone JB24-42) | WB, IHC, FC | Rabbit IgG |
| ZG-0651J | Rabbit Anti-EPAS1 Recombinant Antibody (clone 6A9) | ELISA, IF | Rabbit IgG |
| VS7-0425-WR35 | Mouse Anti-EPAS1 Recombinant Antibody (clone 1H3E12) | IHC, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC359 | CytoStream™ Rabbit Anti-EPAS1 Recombinant Antibody (VS13-YC359) | WB, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2303 | Anti-EPAS1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2304 | Anti-Mouse EPAS1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2305 | Anti-Bovine EPAS1 Immunohistochemistry Kit | IHC |
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