Anti-EPCAM immunotoxin VB4-845 (scFv)-Bouganin (CAT#: AGTO-G021O)

This immunotoxin was achieved by conjugating/fusing the Anti-EPCAM scFv to Bouganin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to tumor cells. Once bind, the toxin enters cells by receptor-mediated endocytosis and enzymatically damages ribosomes, ultimately leading to cell death. It was designed for treatment of Head and neck cancer.

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Specifications

  • Host Species
  • Mouse
  • Species Reactivity
  • Human
  • Clone
  • VB4-845
  • Molecule Class
  • scFv-Bouganin
  • Toxin Description
  • Bouganin is a type-I ribosome inactivating protein (RIP) that was recently purified from leaves of Bougainvillea spectabilis Willd. Like all type-I RIPs, bouganin is a RNA glycosidase with deadenylating activity. Bouganin was characterized as a single-chain protein with a molecular mass of 26 200 Da and its mature protein consists of 250 amino acids. Furthermore, it was found that bouganin is probably the least toxic RIP known based on protein inhibiting studies in cell lines. It was observed that the bouganin concentrations inhibiting protein synthesis of human cell lines were much higher than the concentrations required to inhibit protein synthesis in a rabbit reticulocyte lysate assay, a characteristic common to all type-I RIPs.
  • Applications
  • This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
  • Related Disease
  • Head and neck cancer

Target

  • Alternative Names
  • EPCAM; epithelial cell adhesion molecule; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated antigen; tumor-associated calcium signal transducer 1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); Bouganin

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "EPCAM"

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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