Anti-Human EPHA3 Recombinant Antibody (KB004) (CAT#: TAB-237CL)

Figure 1 EphA3 is expressed in stromal and vascular tissues of human tumors and mouse xenografts.

IHC of corresponding tumor xenografts to detect EphA3 (IIIA4 mAb, KB004). Scale bars, 50 mm. Insets show whole tumor, and magnified areas are indicated by boxes; scale bars, 1 mm.

Vail, M. E., Murone, C., Tan, A., Hii, L., Abebe, D., Janes, P. W., ... & Yarranton, G. (2014). Targeting EphA3 inhibits cancer growth by disrupting the tumor stromal microenvironment. Cancer research, 74(16), 4470-4481.

Figure 2 ChIIIA4 disrupts integrity and function of tumor vessels.

Subcutaneous DU145 tumors, exposed on skin flaps, imaged by intravital multiphoton microscopy.

Vail, M. E., Murone, C., Tan, A., Hii, L., Abebe, D., Janes, P. W., ... & Yarranton, G. (2014). Targeting EphA3 inhibits cancer growth by disrupting the tumor stromal microenvironment. Cancer research, 74(16), 4470-4481.

Figure 3 ChIIIA4 triggers cytoskeletal contraction, EphA3 phosphorylation, and apoptosis of tumor-derived EphA3⁺ MSCs.

α-PY-EphA3 immunoblot analysis of αEphA3 IPs from cells treated with combined chIIIA4/ephrin-A5. Phosphorylation in EphA3/HEK293T cells is shown for comparison.

Vail, M. E., Murone, C., Tan, A., Hii, L., Abebe, D., Janes, P. W., ... & Yarranton, G. (2014). Targeting EphA3 inhibits cancer growth by disrupting the tumor stromal microenvironment. Cancer research, 74(16), 4470-4481.

Figure 4 Stromal and perivascular EphA3 expression in regenerating human endometrium.

Flow cytometry of endometrial stromal cell (eSC) samples testing co-expression of EphA3 and indicated cell surface markers; fractions of positively stained cells are indicated in each quadrant.

To, C., Farnsworth, R. H., Vail, M. E., Chheang, C., Gargett, C. E., Murone, C., ... & Lackmann, M. (2014). Hypoxia-controlled EphA3 marks a human endometrium-derived multipotent mesenchymal stromal cell that supports vascular growth. PloS one, 9(11), e112106.

Figure 5 EphA3 activation triggers phosphorylation, cell contraction, cell repulsion and cell-cell segregation of EphA3⁺ eMSCs.

Organoids formed by EphA3+ eMSC (red) and TEC (green) 3D co-cultures in Matrigel; nuclei stained with DAPI (blue). Treatments (.18 h) were: cross-linked (X-lkd) chimeric (ch)IIIA4 mAb.

To, C., Farnsworth, R. H., Vail, M. E., Chheang, C., Gargett, C. E., Murone, C., ... & Lackmann, M. (2014). Hypoxia-controlled EphA3 marks a human endometrium-derived multipotent mesenchymal stromal cell that supports vascular growth. PloS one, 9(11), e112106.

Figure 1 Anti-Human EPHA3 Recombinant Antibody (TAB-237CL) in WB

Lane M: Marker
Lane 1: Recombinant human EphA3 Protein (0.1 μg)
Lane 2: Recombinant human EphA3 Protein (0.3 μg)
Lane 3: Recombinant human EphA3 Protein (0.6 μg)

Figure 2 Anti-Human EPHA3 Recombinant Antibody (TAB-237CL) in ELISA

ELISA analysis of TAB-237CL was performed by coating with recombinant human EphA3 Protein (His Tag).

Figure 3 Anti-Human EPHA3 Antibody (TAB-237CL) in SDS-PAGE

SDS-PAGE analysis of TAB-237CL in reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 4 Anti-Human EPHA3 Antibody (TAB-237CL) in HPLC

The purity of TAB-237CL was greater than 95% as determined by SEC-HPLC.