How does this immunotoxin work?

The immunotoxin consists of an Anti-ERBB2 scFv fused to Pseudomonas exotoxin (PE). The scFv targets ERBB2 on tumor cells, mediating entry via endocytosis. Inside the cell, the PE toxin inhibits protein synthesis by inactivating elongation factor 2, leading to apoptosis.

What is the intended use of this product?

This product is designed for research use in cytotoxicity assays, functional assays, and immunotherapy studies. It specifically targets cells overexpressing ERBB2, such as SK-OV-3 tumor xenografts, and is useful for investigating targeted cancer treatments.

Anti-ERBB2 immunotoxin 4D5 (scFv)-PE

CAT#: AGTO-G022E

This immunotoxin was achieved by conjugating/fusing the Anti-ERBB2 scFv to Pseudomonas exotoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to tumor cells. Once bind, the toxin enters cells by receptor-mediated endocytosis. Then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. It was designed for treating SK-OV-3 tumor xenografts.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1060/169_D12_1_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1338/if_selected.jpg

Normal Tissue Figure 3 High expression in esophagus

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1383/4682_B_5_1_val_selected.jpg

Normal Tissue Figure 4 IHC staining of human rectum

Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/ihc_selected.jpg

Normal Tissue Figure 5 IHC staining of human urinary bladder

Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/ihc_selected.jpg

Normal Tissue Figure 6 Kidney

Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/1054_A_7_5.jpg

Normal Tissue Figure 7 Breast

Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/45831_B_3_4.jpg

RNA Expression Figure 8 RNA cell line category: Group enriched (OE19, SK-BR-3)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141736-ERBB2

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Specifications

Host Species
Mouse

Species Reactivity
Human

Clone
4D5

Molecule Class
scFv-PE

Toxin Description
Pseudomonas aeruginosa exotoxin (Pseudomonas Aeruginosa Exotoxin A, PE) is a cell toxin produced by P. aeruginosa. Divided into three domains in space. DomainIII (s 405~613 amino acids) having ADP ribosylation activity, can inhibit protein synthesis, PE on toxicity mechanisms of cell depends on its Domainlll. After PE molecules into mammals, Domain Ia and widely distributed in mammalian cells α 2 macroglobulin receptor binding was endocytic, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis.

Applications
This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.

Related Disease
SK-OV-3 tumor xenografts

Target

Alternative Names
ERBB2; erb-b2 receptor tyrosine kinase 2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu; receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; human epidermal growth factor receptor 2; neuro/glioblastoma derived oncogene homolog; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; Pseudomonas exotoxin; Pseudomonas exotoxin A; Pseudomonas aeruginosa exotoxin; Pseudomonas aeruginosa exotoxin A; PE; PE38; PE40; ETA; PE33; PE48

Gene ID
2064

UniProt ID
P04626

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Breast cancer biomarkers at key points during disease progression

Potent Cytotoxicity In SK-OV-3

This immunotoxin construct is fascinating. We tested its cytotoxic effects on SK-OV-3 tumor xenograft derived cells, and it showed significant activity. The fusion of the 4D5 scFv with Pseudomonas exotoxin A works exactly as described for functional assays in oncology research.

Effective Receptor-Mediated Endocytosis

We utilized this product to study the internalization pathways of ERBB2. The toxin component effectively inhibited protein synthesis upon entry, confirming successful receptor-mediated endocytosis. It is a robust tool for developing targeted immunotherapy strategies in a research setting.

Q&As

How does this immunotoxin work?

A: The immunotoxin consists of an Anti-ERBB2 scFv fused to Pseudomonas exotoxin (PE). The scFv targets ERBB2 on tumor cells, mediating entry via endocytosis. Inside the cell, the PE toxin inhibits protein synthesis by inactivating elongation factor 2, leading to apoptosis.
What is the intended use of this product?

A: This product is designed for research use in cytotoxicity assays, functional assays, and immunotherapy studies. It specifically targets cells overexpressing ERBB2, such as SK-OV-3 tumor xenografts, and is useful for investigating targeted cancer treatments.

View the frequently asked questions answered by Creative Biolabs Support.

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Related Diseases

Bladder Cancer

Non-small Cell Lung Cancer

Related Signaling Pathways

ErbB Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - AGTO-G022E.pdf

MSDS

MSDS - AGTO-G022E.pdf

COA

Certificate of Analysis Lookup

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