Hi-Affi™ Rabbit Anti-ETV3 Recombinant Antibody (clone DS1209AB)
CAT#: MOR-1209
Recombinant Rabbit anti-ETV3 monoclonal antibody for WB, FC, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4794/91_F2_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73211/1843_G10_31_selected.jpg
Normal Tissue
Figure 3 IHC staining of human esophagus
Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4794/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4794/13944_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4794/13944_A_9_3.jpg
Normal Tissue
Figure 6 Pancreas
Exocrine glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Pancreatic endocrine cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4794/13944_A_1_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Cells in tubules Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4794/13944_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4794/13944_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4794/13944_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117036-ETV3
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Specifications
- Immunogen
- Synthetic peptide corresponding to residues in Human ETV3.
- Host Species
- Rabbit
- Derivation
- Phage display library
- Type
- Rabbit IgG
- Specificity
- Human ETV3
- Species Reactivity
- Mouse, Rat, Human
- Clone
- DS1209AB
- Applications
- WB, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Superior WB Results
We tried this Hi-Affi™ Rabbit Anti-ETV3 antibody for Western Blotting. The signal was very clean and specific for ETV3 compared to other antibodies. The recombinant rabbit monoclonal technology really delivers on specificity.
Excellent for Flow Cytometry
This clone DS1209AB worked great in Flow Cytometry. It specifically labeled cells expressing ETV3. We appreciate the high affinity and the fact that it is not susceptible to cell-line drift.
Q&As
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What technology is used for this antibody?
A: This is a Hi-Affi™ antibody, developed using recombinant technology (such as phage display) to ensure high specificity and sensitivity, avoiding traditional ascitic fluid methods.
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What applications are supported?
A: This Recombinant Rabbit Anti-ETV3 antibody (clone DS1209AB) is supported for use in Western Blot (WB) and Flow Cytometry (FC).
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "ETV3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2370MZ | Recombinant Mouse Anti-Human ETV3 Antibody (clone FUW4G5E20) | ICC, IF, WB | Mouse antibody |
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