Anti-EpCAM Antibody-Cytokine Fusion Protein, dI-IgG (FN>AQ)-ala (huKS)-IL2 (D20T) (CAT#: ACFP-SH071)
This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-EpCAM dI-IgG (FN>AQ)-ala to IL2 (D20T). It was expressed in CHO and purified with affinity chromatography. The immunocytokine retains the ability to bind the EpCAM as well as the biological activity of IL2. The Ig-moiety for this IL-2 (D20T) fusion protein was derived from the constant region of an IgG γ4 subclass, which in addition retained features of the IgG γ1 hinge. Furthermore, mutations that remove potential T-cell epitopes were introduced. Additionally, this fusion protein contains the substitution from asparagine to glutamine, which eliminates the N-glycosylation site in Fc. The concomitant substitution of a phenylalanine to alanine removes the potential T-cell epitope. This immunocytokine was designed for treating solid tumor.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67463/1592_H11_2_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67463/1592_H11_2_selected.jpg
Figure 2 Thyroid gland
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_B_2_5.jpg
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_B_2_5.jpg
Figure 3 Colon
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_A_9_3.jpg
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_A_9_3.jpg
Figure 4 Kidney
(Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_A_8_5.jpg
(Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_A_8_5.jpg
Figure 5 RNA cell line category: Cell line enhanced (A-431, CACO-2, CAPAN-2, OE19, RPTEC TERT1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000119888-EPCAM
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000119888-EPCAM
Figure 5 RNA cell line category: Cell line enhanced (A-431, CACO-2, CAPAN-2, OE19, RPTEC TERT1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000119888-EPCAM
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000119888-EPCAM
Specifications
- Target
- EpCAM
- Molecule Class
- L Kappa; dI-IgG4 (FN>AQ)-ala-IL2 (D20T)
- Species Reactivity
- Human
- Expression Host
- CHO
- Clone
- huKS
- Related Disease
- Solid tumors
- Cytokine 1
- IL2 (D20T)
- Cytokines 1 Description
- Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 is part of the body's natural response to microbial infection, and in discriminating between foreign ("non-self") and "self". IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes.
- Cytokine 1 Species
- Human
- Applications
- This product can be used therapeutically to deliver biologically active ligands to a desired tissue.
Target
- Alternative Names
- EPCAM; epithelial cell adhesion molecule; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated antigen; tumor-associated calcium signal transducer 1; major gastrointestinal tumor-associated protein GA733-2; membrane component; chromosome 4; surface marker (35kD glycoprotein)
- Gene ID
- 4072
- UniProt ID
- P16422
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|---|---|---|---|
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