Anti-Human JAM-A Recombinant Antibody (TAB-0606CL) (CAT#: TAB-0606CL)
This recombinant antibody is a mouse monoclonal antibody that specifically binds to human JAM-A. A KD of 22 pM of this antibody for the human JAM-A protein was determined under indicated experimental conditions. This purified antibody product can be potentially used in the immunoassays such as IF, IHC, FC, and WB in treatment research of various cancers especially lung cancer and breast cancer.
Specific Inquiry
WB
Figure 1 Western blot analyses using the Mab 6F4 and a commercially available polyclonal anti-JAM-A antibody.
western blot approach 72 hr after transfection.
Goetsch, L., Haeuw, J. F., Beau‐Larvor, C., Gonzalez, A., Zanna, L., Malissard, M., ... & Corvaia, N. (2013). A novel role for junctional adhesion molecule‐A in tumor proliferation: Modulation by an anti‐JAM‐A monoclonal antibody. International journal of cancer, 132(6), 1463-1474.
FC
Figure 2 Flow cytometry evaluation of the Mab 6F4 binding on wild type CHO (CHO) and transiently hJAM-A transfected CHO cells (CHO-hJAM-Aþ).
The Mab 6F4 only recognized transfected CHO cells.
Goetsch, L., Haeuw, J. F., Beau‐Larvor, C., Gonzalez, A., Zanna, L., Malissard, M., ... & Corvaia, N. (2013). A novel role for junctional adhesion molecule‐A in tumor proliferation: Modulation by an anti‐JAM‐A monoclonal antibody. International journal of cancer, 132(6), 1463-1474.
IHC
Figure 3 IHC stainings were performed on detecting JAM-A expression on tumor tissues.
Normal tissues from breast and lung expressed low levels of JAM-A compared to tumor tissues.
Goetsch, L., Haeuw, J. F., Beau‐Larvor, C., Gonzalez, A., Zanna, L., Malissard, M., ... & Corvaia, N. (2013). A novel role for junctional adhesion molecule‐A in tumor proliferation: Modulation by an anti‐JAM‐A monoclonal antibody. International journal of cancer, 132(6), 1463-1474.
IF
Figure 4 Representative immunofluorescence for membrane JAM-A expression after treatment of A431 cells with the Mab 6F4.
JAM-A expression level on cell surface was determined by immunofluorescence staining and visualized by confocal microscopy.
Goetsch, L., Haeuw, J. F., Beau‐Larvor, C., Gonzalez, A., Zanna, L., Malissard, M., ... & Corvaia, N. (2013). A novel role for junctional adhesion molecule‐A in tumor proliferation: Modulation by an anti‐JAM‐A monoclonal antibody. International journal of cancer, 132(6), 1463-1474.
Specifications
- Immunogen
- MCF-7 cell
- Host Species
- Mouse
- Specificity
- Human
- Clone
- 6F4
- Applications
- IF, IHC, FC, WB
- Related Disease
- Lung cancer and breast cancer
Applications
- Application Notes
- The F11R antibody has been reported in applications of Western Blot, Immunofluorescence, Immunohistochemistry, Flow Cytometry. IF:Cells were treated for 48 hr with 10 lg mL1 of either the isotype control or the Mab 6F4. JAM-A expression level on cell surface was determined by immunofluorescence staining and visualized by confocal microscopy. JAM-A was revealed using a secondary antibody conjugated with Alexa Fluor 488 (green) and nuclei were counterstained with DRAQ5TM (blue).
Target
- Alternative Names
- F11R; F11 receptor; JAM; KAT; JAM1; JAMA; JCAM; CD321; PAM-1; junctional adhesion molecule A; platelet F11 receptor; platelet adhesion molecule 1; junctional adhesion molecule 1
- Gene ID
- 50848
- UniProt ID
- Q6FIB4
- Long Name
- F11 Receptor
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone 6F4"
See other products for "F11R"
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-914-VHH | Recombinant Anti-Human F11R VHH Single Domain Antibody | ICC, FC, WB, FUNC | Llama VHH |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1592z | Mouse Anti-F11R Recombinant Antibody (clone 26C10) | WB, FC, IF, IP, FuncS | Mouse IgG1 |
| MOB-2149MZ | Recombinant Mouse Anti-Human F11R Antibody (clone N.Bc.G22) | FC, IP, WB | Mouse antibody |
| HPAB-0298-CN | Human Anti-F11R Recombinant Antibody (clone Hz6F4-2) | ELISA, WB, IF | Humanized IgG4, κ |
| MRO-0888-CN | Rabbit Anti-F11R Recombinant Antibody (clone CBACN-334) | WB, IHC | Rabbit IgG |
| VS3-WK1232 | Rabbit Anti-F11R Recombinant Antibody (VS3-WK1232) | WB, IHC | Rabbit IgG |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0606CL-S(P) | Anti-Human F11R Recombinant Antibody scFv Fragment (6F4) | ELISA | |
| TAB-0606CL-F(E) | Anti-Human F11R Recombinant Antibody Fab Fragment (6F4) | ELISA |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1223 | Hi-Affi™ Rabbit Anti-F11R Recombinant Antibody (clone DS1223AB) | WB, IHC-P | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0298-CN-S(P) | Human Anti-JAM-A Recombinant Antibody; scFv Fragment (HPAB-0298-CN-S(P)) | ELISA, WB, IF | Humanized scFv |
| HPAB-0137-FY-S(P) | Recombinant Human Anti-F11R Antibody scFv Fragment (Hz6F4-2) | In vivo activity | Human scFv |
| HPAB-0880-FY-F(E) | Mouse Anti-F11R Recombinant Antibody (clone 6F4); scFv Fragment | Activ | Humanized scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0297-CN-F(E) | Human Anti-JAM-A Recombinant Antibody; Fab Fragment (HPAB-0297-CN-F(E)) | ELISA, WB, IF | Chimeric (mouse/human) Fab |
| HPAB-0298-CN-F(E) | Human Anti-F11R Recombinant Antibody; Fab Fragment (HPAB-0298-CN-F(E)) | ELISA, WB, IF | Humanized Fab |
| HPAB-0137-FY-F(E) | Recombinant Human Anti-F11R Antibody Fab Fragment (Hz6F4-2) | In vivo activity | Human Fab, κ |
| HPAB-0171CQ-F(E) | Rat Anti-F11r Recombinant Antibody (clone MAb BV12); Fab Fragment | WB, IP, IF | Rat Fab |
| HPAB-0880-FY-S(P) | Mouse Anti-F11R Recombinant Antibody (clone 6F4); Fab Fragment | Activ | Humanized Fab |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for TAB-0606CL. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.