Human Anti-FAP Recombinant Antibody; Fab Fragment (TAB-024WM-F(E))

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Q&As

1. How does the Fab fragment perform in immunoassays for FAP?

   A: We have validated that the Human Anti-FAP Fab Fragment performs well in ELISA, Flow Cytometry, and Immunofluorescence applications by specifically binding to FAP. Its recombinant design and high purity, as highlighted in the product details, allow for consistent detection and quantification of FAP in various research models.

2. What benefits does the recombinant design offer?

   A: We believe the recombinant design of the anti-FAP Fab fragment offers improved specificity and batch-to-batch consistency. Detailed product information, including purification data and storage recommendations, supports reproducible performance in immunoassays. These features help researchers reliably detect FAP in studies involving fibroblast activation.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

1. Sitia, Leopoldo, et al. "Selective targeting of cancer-associated fibroblasts by engineered H-ferritin nanocages loaded with navitoclax." Cells 10.2 (2021): 328. https://doi.org/10.3390/cells10020328
   This research investigates the development of engineered H-Ferritin (HFn) nanocages for targeted delivery of navitoclax (Nav) to cancer-associated fibroblasts (CAFs) in the tumor microenvironment. The study demonstrates that by functionalizing HFn nanocages with fibroblast activation protein (FAP) antibody fragments, they achieved significantly higher binding with FAP-positive CAFs compared to FAP-negative cancer cells. The navitoclax-loaded nanocages (HNav-FAP) showed enhanced cytotoxicity specifically in FAP-positive cells while maintaining the drug's pro-apoptotic mechanism of action. Importantly, in vivo experiments confirmed that HFn-FAP successfully reached tumors and targeted CAFs after intravenous administration in a mouse model of triple-negative breast cancer.
   Creative Biolabs provided FAP antibody fragments (CAT#: TAB-024WM-F(E)) that were used to functionalize the HFn nanocages. These antibody fragments enabled the selective targeting of CAFs over cancer cells by recognizing FAP, a cell surface glycoprotein highly expressed in the stroma surrounding epithelial cancers but not in healthy tissues. This targeting capability was fundamental to the study's success in developing a promising therapeutic approach that could enhance specific drug delivery into CAFs while overcoming navitoclax's limitations of hydrophobicity and toxicity, potentially opening new avenues for tumor microenvironment-focused cancer therapy.

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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