Human Anti-FAP Recombinant Antibody; Fab Fragment (TAB-024WM-F(E)) (CAT#: TAB-024WM-F(E))
This product is a recombinant human antibody Fab Fragment that recognizes FAP. The antibody was purified by affinity chromatography.
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SDS-PAGE
-1.png)
Figure 1 Anti-FAP Fab Fragment (TAB-024WM-F(E)) in SDS-PAGE
SDS-PAGE analysis of TAB-024WM-F(E) in reduced (lane 1) conditions.
SEC-HPLC
-2.png)
Figure 2 Anti-FAP Fab Fragment (TAB-024WM-F(E)) in HPLC
The purity of TAB-024WM-F(E) was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8x300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.3
Detection: UV280 nm
Injection: 10 µl
Specifications
- Immunogen
- Human or murine fibroblast activating protein (FAP)
- Host Species
- Human
- Type
- Human Fab
- Specificity
- Human
- Species Reactivity
- Human, Mouse, Monkey
- Applications
- Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay; Flow Cytometry; Immunofluorescence
- Related Disease
- Disease characterized by expression of FAP
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- FAPA; SIMP; DPPIV; FAP; fibroblast activation protein alpha
- Gene ID
- 2191
- UniProt ID
- Q12884
Related Resources
Sitia, Leopoldo, et al. "Selective targeting of cancer-associated fibroblasts by engineered H-ferritin nanocages loaded with navitoclax." Cells 10.2 (2021): 328. https://doi.org/10.3390/cells10020328
This research investigates the development of engineered H-Ferritin (HFn) nanocages for targeted delivery of navitoclax (Nav) to cancer-associated fibroblasts (CAFs) in the tumor microenvironment. The study demonstrates that by functionalizing HFn nanocages with fibroblast activation protein (FAP) antibody fragments, they achieved significantly higher binding with FAP-positive CAFs compared to FAP-negative cancer cells. The navitoclax-loaded nanocages (HNav-FAP) showed enhanced cytotoxicity specifically in FAP-positive cells while maintaining the drug's pro-apoptotic mechanism of action. Importantly, in vivo experiments confirmed that HFn-FAP successfully reached tumors and targeted CAFs after intravenous administration in a mouse model of triple-negative breast cancer.
Creative Biolabs provided FAP antibody fragments (CAT#: TAB-024WM-F(E)) that were used to functionalize the HFn nanocages. These antibody fragments enabled the selective targeting of CAFs over cancer cells by recognizing FAP, a cell surface glycoprotein highly expressed in the stroma surrounding epithelial cancers but not in healthy tissues. This targeting capability was fundamental to the study's success in developing a promising therapeutic approach that could enhance specific drug delivery into CAFs while overcoming navitoclax's limitations of hydrophobicity and toxicity, potentially opening new avenues for tumor microenvironment-focused cancer therapy.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-211 | Anti-Human FAP Recombinant Antibody (TAB-211) | ELISA, IP, FC, FuncS, Neut, IF, WB | IgG1 - kappa |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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