Anti-Human FAT1 Recombinant Antibody (198.3)
CAT#: TAB-030WM
A novel tumor targeting antibody directed against colon expressed FAT1 (clone 198.3) potentially used for intracellular negative gold nanoparticles delivery, holding significant potential to improve the treatment of cancer.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human rectum
Immunohistochemical staining of human rectum shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23882/52199_A_5_3_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1869/7555_B_8_5.jpg
Normal Tissue
Figure 3 Small intestine
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1869/7834_A_6_2.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1869/7834_A_7_3.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (fHDF/TERT166, LHCN-M2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000083857-FAT1
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Specifications
- Immunogen
- Recombinant FAT1 (rFAT1)
- Host Species
- Mouse
- Specificity
- Human
- Species Reactivity
- Human
- Clone
- 198.3
- Applications
- FC, WB, IHC
- Related Disease
- Colorectal cancer
Applications
- Application Notes
- This antibody has been tested for use in Flow Cytometry, Western Blot, Immunohistochemistry. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
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Ideal Concentration
The ready-to-use concentration was perfect for our studies. We were spared a great deal of time and expense in not having to dilute or change.
Superb Detection
The antibody is robust and produces reliable results even after several months in storage. It is extremely recommended to aliquot in order to maintain its effectiveness.
Q&As
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What tactics can improve the stability and lifetime of antibodies?
A: To avoid recurrent freeze-thaw cycles, use stabilizers like glycerol, handle aseptically to avoid contamination, and so on. Store in the authorized circumstances.
-
How can I deal with antibody performance variability?
A: Perform tests for quality control, standardize sample preparation, adhere to established protocols, and look into any batch-to-batch variations or performance-affecting environmental conditions.
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How should samples be ready in order to apply an antibody label?
A: Make that samples are adequately lysed and that all contaminating agents are eliminated. In order to reduce non-specific binding, standard methods sometimes include the use of blocking agents in addition to buffers like PBS.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-030WM, RRID: AB_3111764)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone 198.3"
- CAT
- Product Name
See other products for "FAT1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4061z | Mouse Anti-FAT1 Recombinant Antibody (clone 44H5) | WB, ELISA | Mouse IgG1, κ |
| MRO-2136-CN | Rabbit Anti-FAT1 Polyclonal Antibody (MRO-2136-CN) | WB | Rabbit IgG |
| HPAB-0124-WJ | Mouse Anti-FAT1 Recombinant Antibody (HPAB-0124-WJ) | IHC, ELISA, WB | Mouse IgG2a, κ |
| VS3-WK959 | Mouse Anti-FAT1 Recombinant Antibody (VS3-WK959) | WB | Mouse IgG |
| MOB-328YC | Rabbit Anti-FAT1 Recombinant Antibody (MOB-328YC) | ELISA, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-030WM-S(P) | Anti-Human FAT1 Recombinant Antibody scFv Fragment (198.3) | WB, IHC, FC | |
| TAB-030WM-F(E) | Anti-Human FAT1 Recombinant Antibody Fab Fragment (198.3) | WB, IHC, FC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0124-WJ-S(P) | Mouse Anti-FAT1 Recombinant Antibody; scFv Fragment (HPAB-0124-WJ-S(P)) | IHC, ELISA, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0124-WJ-F(E) | Mouse Anti-FAT1 Recombinant Antibody; Fab Fragment (HPAB-0124-WJ-F(E)) | IHC, ELISA, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC347 | Recombinant Anti-FAT1 Vesicular Antibody, EV Displayed (VS-0425-YC347) | ELISA, FC, Cell-uptake |
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