Human Anti-FGFR3 Recombinant Antibody (clone R3Mab)
CAT#: PABL-474
Recombinant Human Antibody (R3Mab) is capable of binding to FGFR3, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-FGFR3 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-FGFR3 proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody inhibited not only WT FGFR3, but also various mutants of the receptor.
Published Data
Tested Data
Gene Expression
Figure 1 Blocking of R3Mab to FGF-FGFR3 interaction.
(A) Selective binding of human FGFR3 by R3Mab. Human FGFR1–4 Fc chimeric proteins were immobilized and incubated with increasing amounts of R3Mab. Specific binding was detected using an anti-human Fab antibody. (B and C) Blocking of FGF1 binding to human FGFR3-IIIb (B) or -IIIc (C) by R3Mab. Specific binding was detected by using a biotinylated FGF1-specific polyclonal antibody. (D and E) Blocking of FGF9 binding to human FGFR3-IIIb (D) or -IIIc (E) by R3Mab. Specific binding was detected by using a biotinylated FGF9-specific polyclonal antibody. Error bars represent SEM and are sometimes smaller than symbols.
Qing, J., Du, X., Chen, Y., Chan, P., Li, H., Wu, P., ... & Ross, S. (2009). Antibody-based targeting of FGFR3 in bladder carcinoma and t (4; 14)-positive multiple myeloma in mice. The Journal of clinical investigation, 119(5), 1216-1229.
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SDS-PAGE
Figure 1 Human Anti-FGFR3 Recombinant Antibody (PABL-474) in SDS-PAGE
Lane 1: PABZ-474, 2 ug, Non-reducing
Lane 2: PABZ-474, 2 ug, Reducing
Lane M: Protein Marker
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67204/1419_D10_2_selected.jpg
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Specifications
- Immunogen
- Human fibroblast growth factor receptor 3
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human FGFR3
- Species Reactivity
- Human
- Clone
- R3Mab
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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(Creative Biolabs Cat# PABL-474, RRID: AB_3111659)
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Related Diseases
Bladder Cancer
EGFR Tyrosine Kinase Inhibitor Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| CAT | Product Name | Application | Type |
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| PSBW-162 | Human Anti-FGFR3 Recombinant Antibody (clone R3); scFv Fragment | Neut, ELISA, FC | Human scFv |
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| HPAB-0158-YC-F(E) | Mouse Anti-FGFR3 Recombinant Antibody; Fab Fragment (HPAB-0158-YC-F(E)) | ELISA, FC | Mouse Fab |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-471 | Human Anti-FGFR3 Recombinant Antibody (clone R3Mab); scFv Fragment | ELISA | Human scFv |
| HPAB-0150-WJ-S(P) | Human Anti-FGFR3 Recombinant Antibody; scFv Fragment (HPAB-0150-WJ-S(P)) | ELISA | Human scFv |
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| TAB-062WM-F(E) | Mouse Anti-FGFR3 Recombinant Antibody; Fab Fragment (TAB-062WM-F(E)) | ELISA, FC, Neut, WB | Mouse Fab |
| TAB-063WM-F(E) | Mouse Anti-FGFR3 Recombinant Antibody; Fab Fragment (TAB-063WM-F(E)) | ELISA, FC, Neut, WB | Mouse Fab |
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| NEUT-838CQ | Rat Anti-Fgfr3 Recombinant Antibody (clone CBL548) | Neut | Rat IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| VS-0525-XY2544 | Anti-Mouse FGFR3 Immunohistochemistry Kit | IHC | |
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