Anti-FOLR1 Intrabody [Tat] (IAB-B029(T))
Recombinant anti-FOLR1 intrabody in fusion with HIV Tat works within cells to bind FOLR1, which is selectively overexpressed in 90% of nonmucinous ovarian carcinomas, expressed in CHO cells. FR expression is associated with tumor progression and affects cell proliferation and the intracellular expression of single-chain (sc) antibodiess (intrabody) can downmodulate membrane expression of FR in ovary cancer cells.
Specifications
- Immunogen
- human folate receptor 1 (adult)
- Host
- mouse
- Type
- scFv-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Species Reactivity
- human
- Applications
- Antibody assay: Western blot; Immunocytochemistry; Neutralization; Functional studies
Target
- Alternative Names
- FOLR1; folate receptor 1 (adult); FBP; FOLR; folate receptor alpha; FR-alpha; KB cells FBP; folate binding protein
- Entrez Gene ID
- 2348
- UniProt ID
- A0A024R5H1
For lab research use only, not for diagnostic, therapeutic or any in vivo human use.
Our customer service representatives are available 24 hours a day, from Monday to Sunday. Contact Us
Tel: 1-631-381-2994
Fax: 1-631-207-8356
Email: