Human Anti-FAS Recombinant Antibody (clone HFE7A) (CAT#: PABL-094)
Recombinant Human Antibody (HFE7A) is capable of binding to Fas, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-Fas mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-Fas mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
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Figure 1 Binding Assay to Human Fas-AIC2.
The symbols correspond to h-HFE7A α- (◇), β- (□), γ- (〇), δ- (△), and ζ- (▼). Chimeric HFE7A (■) was used as the positive control. Human IgG1 (●) was used as the negative control. Similar results were obtained with three independent experiments.
Haruyama, H., Ito, S., Miyadai, K., Takahashi, T., Kawaida, R., Takayama, T.,... & Ichikawa, K. (2002). Humanization of the mouse anti-Fas antibody HFE7A and crystal structure of the humanized HFE7A Fab fragment. Biological and Pharmaceutical Bulletin, 25(12), 1537-1545.
Figure 2 Inhibition Assay against the Binding between m-HFE7A and Human Fas-AIC2.
The symbols correspond to h-HFE7A α- (◇), β- (□), γ- (〇), δ- (△), and ζ- (▼). Chimeric HFE7A (■) was used as the positive control. Human IgG1 (●) was used as the negative control. Similar results were obtained with three independent experiments.
Haruyama, H., Ito, S., Miyadai, K., Takahashi, T., Kawaida, R., Takayama, T.,... & Ichikawa, K. (2002). Humanization of the mouse anti-Fas antibody HFE7A and crystal structure of the humanized HFE7A Fab fragment. Biological and Pharmaceutical Bulletin, 25(12), 1537-1545.
Figure 3 Apoptosis Induction Assay against WR19L12a Cells.
The symbols correspond to h-HFE7A α- (◇), β- (□), γ- (〇), δ- (△), and ζ- (▼). Chimeric HFE7A (■) was used as the positive control. Human IgG1 (●) was used as the negative control. Similar results were obtained with three independent experiments.
Haruyama, H., Ito, S., Miyadai, K., Takahashi, T., Kawaida, R., Takayama, T.,... & Ichikawa, K. (2002). Humanization of the mouse anti-Fas antibody HFE7A and crystal structure of the humanized HFE7A Fab fragment. Biological and Pharmaceutical Bulletin, 25(12), 1537-1545.
Specifications
- Immunogen
- Human Fas cell surface death receptor
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human FAS
- Species Reactivity
- Human
- Clone
- HFE7A
- Applications
- ELISA, WB, IF, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Inhibition and Apoptosis. For details, refer to published data.
Target
Related Resources
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Maturity Onset Diabetes of the Young
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-094 | Human Anti-FAS Recombinant Antibody (clone HFE7A); Fab Fragment | ELISA, WB, IF, FuncS | Human Fab |
| PFBL-723 | Human Anti-FAS Recombinant Antibody (clone E09); Fab Fragment | WB, IF, FuncS | Human Fab |
| HPAB-0035-YJ-F(E) | Human Anti-FAS Recombinant Antibody (clone h-HFE7A); Fab Fragment | ELISA, Cyt, Apop | Human Fab |
| HPAB-1575WJ-F(E) | Mouse Anti-FAS Recombinant Antibody; Fab Fragment (clone F919-9-18) | ELISA, WB | Mouse Fab |
| HPAB-1576WJ-F(E) | Human Anti-FAS Recombinant Antibody; Fab Fragment (clone F919) | ELISA, WB | Human Fab |
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