Recombinant Mouse Antibody (43.1) is capable of binding to fluorescein's carboxyl phenyl moiety, expressed in Chinese Hamster Ovary cells (CHO). It demonstrates a very high affinity (KD ∼ 70 pM) and a fast association rate (kon ∼ 2 × 10(7) M(-1) s(-1)).
Figure 2 Spectra and fluorescence decay of fluorescein, free and bound to mAb 43. Normalized absorption and emission spectra of free (A) and antibody bound (B) fluorescein. Excitation spectra fully trace absorption spectra in both cases. C: Fluorescence time decay of free and antibody bound fluorescein. To obtain comparable signal for the highly quenched bound form the sample concentration and excitation power were increased. D,E: residuals to the fit with three lifetime components.
Gayda, S., Longenecker, K. L., Judge, R. A., Swift, K. M., Manoj, S., Linthicum, D. S., & Tetin, S. Y. (2016). Three‐dimensional structure, binding, and spectroscopic characteristics of the monoclonal antibody 43.1 directed to the carboxyphenyl moiety of fluorescein. Biopolymers, 105(4), 234-243.
Figure 3 Equilibrium binding plot and thermodynamic parameters of the mAb 43.1 - fluorescein interaction. A: The fluorescein concentration was kept at 20 nM while concentration of the antibody varied when performing equilibrium binding tittrations. Arrhenius plots calculated for the association (B) and dissociation (C) binding reactions and van‘t Hoff plot (D) with the Ka values calculated from the kinetics data.
Gayda, S., Longenecker, K. L., Judge, R. A., Swift, K. M., Manoj, S., Linthicum, D. S., & Tetin, S. Y. (2016). Three‐dimensional structure, binding, and spectroscopic characteristics of the monoclonal antibody 43.1 directed to the carboxyphenyl moiety of fluorescein. Biopolymers, 105(4), 234-243.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
DrMAB-3 | Human Anti-Fluorescein Recombinant Antibody (clone 4-4-20) | WB, FC | Human IgG |
DrMAB-13 | Mouse Anti-Fluorescein Recombinant Antibody (clone 4-4-20) | ELISA, WB, FC | Mouse IgG |
DrMAB-23 | Rat Anti-Fluorescein Recombinant Antibody (clone 4-4-20) | ELISA, WB, FC | Rat IgG |
FAMAB-0113YC | Chicken Anti-Fluorescein Recombinant Antibody (clone 84/85-s-3) | ELISA | Chicken IgG |
FAMAB-0114YC | Chicken Anti-Fluorescein Recombinant Antibody (clone 84/85-F-8) | ELISA | Chicken IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
DrMAB-6 | Human Anti-Fluorescein Recombinant Antibody (clone 4-4-20); Fab Fragment | WB, FC | Human Fab |
PFBL-096 | Mouse Anti-Fluorescein Recombinant Antibody (clone 43.1); Fab Fragment | ELISA, WB, FuncS | Mouse Fab |
PFBZ-046 | Human Anti-Fluorescein Recombinant Antibody (clone 4M5.3); Fab Fragment | FC | Human Fab |
PFBW-046 | Mouse Anti-Fluorescein Recombinant Antibody (clone 9-40); Fab Fragment | WB, IF, FuncS | Mouse Fab |
PFBC-047 | Human Anti-Fluorescein Recombinant Antibody (clone E2); Fab Fragment | WB, FuncS | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBL-096 | Mouse Anti-Fluorescein Recombinant Antibody (clone 43.1); scFv Fragment | ELISA, WB, FuncS | Mouse scFv |
PSBL-472 | Mouse Anti-Fluorescein Recombinant Antibody (clone mAb 4-4-20); scFv Fragment | Neut | Mouse scFv |
FAMAB-0113-YC-S(P) | Chicken Anti-Fluorescein Recombinant Antibody (clone 84/85-s-3); scFv Fragment | ELISA | Chicken scFv |
FAMAB-0114-YC-S(P) | Chicken Anti-Fluorescein Recombinant Antibody (clone 84/85-F-8); scFv Fragment | ELISA | Chicken scFv |
FAMAB-0141CQ-S(P) | Mouse Anti-Fluorescein Recombinant Antibody (clone 18-2-3); scFv Fragment | ELISA | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-037 | Recombinant Mouse Anti-fluorescein Antibody (4-4-20) | WB, ELISA, FuncS, PK | IgG |
PABX-037-F (E) | Recombinant Mouse Anti-fluorescein Antibody Fab Fragment (4-4-20) | WB, ELISA, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-037-S (P) | Recombinant Mouse Anti-fluorescein Antibody scFv Fragment (4-4-20) | WB, ELISA, FuncS | scFv |
There are currently no Customer reviews or questions for PABL-096. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.