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    Recombinant Anti-human G3BP Intrabody [(D-Arg)9] (CAT#: IAB-B016(A))

    Recombinant anti-G3BP intrabody linked to (D-Arg)9 works within cells to bind intracellur antigen human G3BP, expressed in 293T cells. This inhibition is attributed to the blocking of the endogenous G3BP protein.

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    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line U-251 MG
    Figure 1 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/if_selected.jpg
    Figure 4 IF staining of human cell line A-431
    Figure 4 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/27_D12_1_blue_red_green.jpg
    Figure 5 IF staining of human cell line U-2 OS
    Figure 5 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/26_D12_1_blue_red_green.jpg
    Normal Tissue
    Figure 2 IHC staining of human lymph node
    Figure 2 IHC staining of human lymph node
    (Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in germinal center cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/ihc_selected.jpg
    Figure 3 IHC staining of human duodenum
    Figure 3 IHC staining of human duodenum
    (Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/37068/ihc_selected.jpg
    Figure 6 Cerebral cortex
    Figure 6 Cerebral cortex
    (Neuronal cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/151830_B_7_5.jpg
    Figure 7 Colon
    Figure 7 Colon
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Glandular cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/151830_A_9_3.jpg
    Figure 8 Kidney
    Figure 8 Kidney
    (Cells in tubules
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/151830_A_8_5.jpg
    Figure 9 Testis
    Figure 9 Testis
    (Pachytene spermatocytes
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Preleptotene spermatocytes
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Round or early spermatids
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Spermatogonia cells
    Staining: High
    Intensity: Strong
    Quantity:>75%)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/151830_A_4_6.jpg
    Figure 10 Lymph node
    Figure 10 Lymph node
    (Germinal center cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Non-germinal center cells
    Staining: Medium
    Intensity: Strong
    Quantity: <25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/151830_A_9_8.jpg
    RNA Expression
    Figure 11 RNA cell line category: Low cell line specificity
    Figure 11 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000145907-G3BP1

    Specifications

    • Immunogen
    • human GTPase activating protein (SH3 domain) binding protein 1
    • Host Species
    • llama
    • Type
    • vhh-(D-Arg)9
    • Cell Penetrating Peptide
    • (D-Arg)9
      It has been proven that polyarginine peptides composed of nine arginine amino acids (Arg-9) are able to translocate across cells very efficiently. This raises the question if molecular dynamics simulations would show a similar pathway for this highly hydrophilic and charged peptide as predicted for the Tat peptide. The simplicity of Arg-9 over other CPPs also helps isolate the role of the Arg sidechain on the translocation without the effect of other sidechains. Therefore, in this work we study computationally the translocation of Arg-9 through a lipid bilayer and show that the translocation mechanism is the same as for the Tat peptide.
    • Species Reactivity
    • human
    • Applications
    • Antibody assay: Immunofluorescence; Flow Cytometry; Western blot; Functional studies

    Product Property

    • Purity
    • >97%, by SDS-PAGE under reducing conditions
    • Storage
    • Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.

    Target

    • Alternative Names
    • G3BP1; GTPase activating protein (SH3 domain) binding protein 1; G3BP; HDH-VIII; ras GTPase-activating protein-binding protein 1; G3BP-1; GAP binding protein; ATP-dependent DNA helicase VIII; GAP SH3 domain-binding protein 1; RasGAP-associated endoribonuc

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

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    Please select product type
    Intrabody Products
    CAT Product Name Application Type
    IAB-B016(G) Recombinant Anti-human G3BP Intrabody [+36 GFP] WB, CO-IP, FuncS vhh-(+36GFP)
    IAB-B016(T) Recombinant Anti-human G3BP Intrabody [Tat] IF, ELISA, FuncS vhh-Tat

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