Recombinant Mouse Anti-GD2 ganglioside Antibody (3F8) (CAT#: PABL-101)

Recombinant Mouse Antibody (3F8) is capable of binding to GD2 ganglioside, expressed in Chinese Hamster Ovary cells (CHO). It is a murine IgG3 with a moderate affinity for GD2 (KD = 5 nM).

  • Published Data
  • Datasheet
  • MSDS
  • COA

Figure 1 Molecular Dynamics Analyses of Unliganded 3F8

(A and B)Worm plots of germline 3F8 (A) and affinitymature 3F8 (B) illustrating a relatively rigid germline structure that loosens slightly during affinity maturation. CDR loops are labeled, and residuemutations between germline and affinity-mature antibodies are shown as stick representation. (C–H) Histograms of RMSD distribution from the starting conformation for CDR loops during the simulations. Light-chain CDRs 1–3 (C–E, respectively) and heavy-chain CDRs 1–3 (F–H, respectively) illustrate the propensity of mature CDRs to explore the conformational space more liberally.

Sterner, E., Peach, M. L., Nicklaus, M. C., & Gildersleeve, J. C. (2017). Therapeutic antibodies to ganglioside GD2 evolved from highly selective germline antibodies. Cell reports, 20(7), 1681-1691.

Figure 2 3F8 Antibody Direct Cytotoxicity.

The 3F8 antibodies (mature and germline) were capable of promoting direct cytotoxicity against the GD2-presenting M14 melanoma cell lines. These antibodies were incapable of inducing direct cytotoxicity against the colorectal cancer cell line SW620.

Sterner, E., Peach, M. L., Nicklaus, M. C., & Gildersleeve, J. C. (2017). Therapeutic antibodies to ganglioside GD2 evolved from highly selective germline antibodies. Cell reports, 20(7), 1681-1691.

Figure 3 (D and E) Affinity-mature 3F8 (D) and germline 3F8 (E) were also extremely specific for GD2. (F) GQ2 binding was observed in 3F8 at levels greater than 250-fold reduced versus that of GD2.

Sterner, E., Peach, M. L., Nicklaus, M. C., & Gildersleeve, J. C. (2017). Therapeutic antibodies to ganglioside GD2 evolved from highly selective germline antibodies. Cell reports, 20(7), 1681-1691.


Specifications

  • Immunogen
  • Human GD2
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • IgG
  • Specificity
  • Tested positive against native Human GD2
  • Species Reactivity
  • Human
  • Clone
  • 3F8
  • Applications
  • Cyt, FC, ELISA, FuncS

Applications

  • Application Notes
  • The antibody was validated for Cyt. For details, refer to published data.

Target

  • Alternative Names
  • disialoganglioside; GD2

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "GD2"

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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