Mouse Anti-GD2 Recombinant Antibody (clone 3F8) (CAT#: PABL-101)

Recombinant Mouse Antibody (3F8) is capable of binding to GD2 ganglioside, expressed in Chinese Hamster Ovary cells (CHO). It is a murine IgG3 with a moderate affinity for GD2 (KD = 5 nM).


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  • Published Data
  • Tested Data
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Figure 1 Molecular Dynamics Analyses of Unliganded 3F8

Figure 1 Molecular Dynamics Analyses of Unliganded 3F8

(A and B)Worm plots of germline 3F8 (A) and affinitymature 3F8 (B) illustrating a relatively rigid germline structure that loosens slightly during affinity maturation. CDR loops are labeled, and residuemutations between germline and affinity-mature antibodies are shown as stick representation. (C–H) Histograms of RMSD distribution from the starting conformation for CDR loops during the simulations. Light-chain CDRs 1–3 (C–E, respectively) and heavy-chain CDRs 1–3 (F–H, respectively) illustrate the propensity of mature CDRs to explore the conformational space more liberally.

Sterner, E., Peach, M. L., Nicklaus, M. C., & Gildersleeve, J. C. (2017). Therapeutic antibodies to ganglioside GD2 evolved from highly selective germline antibodies. Cell reports, 20(7), 1681-1691.

Figure 2 3F8 Antibody Direct Cytotoxicity.

Figure 2 3F8 Antibody Direct Cytotoxicity.

The 3F8 antibodies (mature and germline) were capable of promoting direct cytotoxicity against the GD2-presenting M14 melanoma cell lines. These antibodies were incapable of inducing direct cytotoxicity against the colorectal cancer cell line SW620.

Sterner, E., Peach, M. L., Nicklaus, M. C., & Gildersleeve, J. C. (2017). Therapeutic antibodies to ganglioside GD2 evolved from highly selective germline antibodies. Cell reports, 20(7), 1681-1691.

Figure 3 (D and E) Affinity-mature 3F8 (D) and germline 3F8 (E) were also extremely specific for GD2. (F) GQ2 binding was observed in 3F8 at levels greater than 250-fold reduced versus that of GD2.

Figure 3 (D and E) Affinity-mature 3F8 (D) and germline 3F8 (E) were also extremely specific for GD2. (F) GQ2 binding was observed in 3F8 at levels greater than 250-fold reduced versus that of GD2.

Sterner, E., Peach, M. L., Nicklaus, M. C., & Gildersleeve, J. C. (2017). Therapeutic antibodies to ganglioside GD2 evolved from highly selective germline antibodies. Cell reports, 20(7), 1681-1691.


Specifications

  • Immunogen
  • Human GD2
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human GD2
  • Species Reactivity
  • Human
  • Clone
  • 3F8
  • Applications
  • FC, ELISA, FuncS

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody was validated for Cytotoxicity. For details, refer to published data.

Target

  • Alternative Names
  • disialoganglioside; GD2

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Single-domain Antibody

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Chimeric Antibody

CAT Product Name Application Type
TAB-731 Anti-GD2 Recombinant Antibody (Dinutuximab) Neut, ELISA, IF, IP, FuncS, FC, ICC IgG1 - kappa

Immunotoxin

CAT Product Name Application Type
AGTO-L018R anti-GD2 immunotoxin 14G2a (IgG)-RTA Cytotoxicity assay, Functional assay

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-731 Afuco™ Anti-GD2 ADCC Recombinant Antibody (Dinutuximab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS, FC ADCC enhanced antibody

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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