Recombinant Mouse Antibody (3F8) is capable of binding to GD2 ganglioside, expressed in Chinese Hamster Ovary cells (CHO). It is a murine IgG3 with a moderate affinity for GD2 (KD = 5 nM).
Figure 1 Molecular Dynamics Analyses of Unliganded 3F8
(A and B)Worm plots of germline 3F8 (A) and affinitymature 3F8 (B) illustrating a relatively rigid germline structure that loosens slightly during affinity maturation. CDR loops are labeled, and residuemutations between germline and affinity-mature antibodies are shown as stick representation. (C–H) Histograms of RMSD distribution from the starting conformation for CDR loops during the simulations. Light-chain CDRs 1–3 (C–E, respectively) and heavy-chain CDRs 1–3 (F–H, respectively) illustrate the propensity of mature CDRs to explore the conformational space more liberally.
Sterner, E., Peach, M. L., Nicklaus, M. C., & Gildersleeve, J. C. (2017). Therapeutic antibodies to ganglioside GD2 evolved from highly selective germline antibodies. Cell reports, 20(7), 1681-1691.
Figure 2 3F8 Antibody Direct Cytotoxicity.
The 3F8 antibodies (mature and germline) were capable of promoting direct cytotoxicity against the GD2-presenting M14 melanoma cell lines. These antibodies were incapable of inducing direct cytotoxicity against the colorectal cancer cell line SW620.
Sterner, E., Peach, M. L., Nicklaus, M. C., & Gildersleeve, J. C. (2017). Therapeutic antibodies to ganglioside GD2 evolved from highly selective germline antibodies. Cell reports, 20(7), 1681-1691.
Figure 3 (D and E) Affinity-mature 3F8 (D) and germline 3F8 (E) were also extremely specific for GD2. (F) GQ2 binding was observed in 3F8 at levels greater than 250-fold reduced versus that of GD2.
Sterner, E., Peach, M. L., Nicklaus, M. C., & Gildersleeve, J. C. (2017). Therapeutic antibodies to ganglioside GD2 evolved from highly selective germline antibodies. Cell reports, 20(7), 1681-1691.
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CAT | Product Name | Application | Type |
---|---|---|---|
NAB-770-sdAb | Recombinant Anti-Human GD2 VHH Single Domain Antibody | Neut, ChiP, IP, FUNC | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-731 | Anti-GD2 Recombinant Antibody (Dinutuximab) | Neut, ELISA, IF, IP, FuncS, FC, ICC | IgG1 - kappa |
CAT | Product Name | Application | Type |
---|---|---|---|
AGTO-L018R | anti-GD2 immunotoxin 14G2a (IgG)-RTA | Cytotoxicity assay, Functional assay |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-304CL | Anti-Human GD2 Recombinant Antibody (hu14.18K322A) | FC, FuncS | Antibody |
PABX-059 | Human Anti-GD2 Recombinant Antibody (PABX-059) | FuncS | IgG1, κ |
PABX-059-F (E) | Human Anti-GD2 Recombinant Antibody; Fab Fragment (PABX-059-F (E)) | FC, ELISA, FuncS | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PABL-100 | Mouse Anti-GD2 Recombinant Antibody (clone 14G2a) | ELISA, WB, FuncS | Mouse IgG |
PABZ-049 | Mouse Anti-GD2 Recombinant Antibody (clone ME36.1) | WB, FuncS | Mouse IgG |
FAMAB-0126YC | Mouse Anti-GD2 Recombinant Antibody (clone 7A4) | ELISA | Mouse IgG3, κ |
HPAB-0701-CN | Human Anti-GD2 Recombinant Antibody (clone AB527) | ELISA, FC, IHC, IF | Human IgM |
HPAB-1030WJ | Human Anti-GD2 Recombinant Antibody (HPAB-1030WJ) | ELISA | Chimeric (mouse/human) IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBZ-049 | Mouse Anti-GD2 Recombinant Antibody (clone ME36.1); Fab Fragment | WB, FuncS | Mouse Fab |
FAMAB-0126-YC-F(E) | Mouse Anti-GD2 Recombinant Antibody (clone 7A4); Fab Fragment | ELISA | Mouse Fab |
HPAB-0701-CN-F(E) | Human Anti-GD2 Recombinant Antibody (clone AB527); Fab Fragment | ELISA, FC, IHC, IF | Human Fab |
HPAB-1030WJ-F(E) | Human Anti-GD2 Recombinant Antibody; Fab Fragment (HPAB-1030WJ-F(E)) | ELISA | Chimeric (mouse/human) Fab |
HPAB-1524WJ-F(E) | Human Anti-GD2 Recombinant Antibody; Fab Fragment (clone KM8138) | ELISA, WB | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBZ-049 | Mouse Anti-GD2 Recombinant Antibody (clone ME36.1); scFv Fragment | WB, FuncS | Mouse scFv |
FAMAB-0126-YC-S(P) | Mouse Anti-GD2 Recombinant Antibody (clone 7A4); scFv Fragment | ELISA | Mouse scFv |
HPAB-0701-CN-S(P) | Human Anti-GD2 Recombinant Antibody (clone AB527); scFv Fragment | ELISA, FC, IHC, IF | Human scFv |
HPAB-1524WJ-S(P) | Human Anti-GD2 Recombinant Antibody; scFv Fragment (clone KM8138) | ELISA, WB | Human scFv |
HPAB-1525WJ-S(P) | Human Anti-GD2 Recombinant Antibody; scFv Fragment (clone KM1138) | ELISA, WB | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-074CQ-S(P) | Human Anti-GD2 Recombinant Antibody; scFv Fragment (TAB-074CQ-S(P)) | ELISA, FC | Human scFv |
TAB-075CQ-S(P) | Human Anti-GD2 Recombinant Antibody; scFv Fragment (TAB-075CQ-S(P)) | ELISA, FC | Human scFv |
TAB-076CQ-S(P) | Human Anti-GD2 Recombinant Antibody; scFv Fragment (TAB-076CQ-S(P)) | ELISA, FC | Human scFv |
TAB-077CQ-S(P) | Human Anti-GD2 Recombinant Antibody; scFv Fragment (TAB-077CQ-S(P)) | ELISA, FC | Human scFv |
TAB-078CQ-S(P) | Human Anti-GD2 Recombinant Antibody; scFv Fragment (TAB-078CQ-S(P)) | ELISA, FC | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
NS-029CN | Mouse Anti-GD2 Recombinant Antibody (clone 5F11) | WB | Mouse IgG |
NS-030CN | Mouse Anti-GD2 Recombinant Antibody (clone 3G6) | WB | Mouse IgG |
NS-029CN-F(E) | Mouse Anti-GD2 Recombinant Antibody (clone 5F11); Fab Fragment | WB | Mouse Fab |
NS-030CN-F(E) | Mouse Anti-GD2 Recombinant Antibody (clone 3G6); Fab Fragment | WB | Mouse Fab |
NS-029CN-S(P) | Mouse Anti-GD2 Recombinant Antibody (clone 5F11); scFv Fragment | WB | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-731 | Afuco™ Anti-GD2 ADCC Recombinant Antibody (Dinutuximab), ADCC Enhanced | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
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