Mouse Anti-GD3 Recombinant Antibody (clone R24) (CAT#: PABC-050)

Recombinant Mouse Antibody (R24) is capable of binding to GD3 (cell-surface tumor antigen disialoganglioside), expressed in Chinese Hamster Ovary cells (CHO). R24 has been shown to prevent tumor development in animal models, as well as to display anti-tumor activity in human clinical trials.


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Figure 1 R24 antibody is recycled to the plasma membrane and released into the culture medium.

Figure 1 R24 antibody is recycled to the plasma membrane and released into the culture medium.

(A) GD3-expressing CHO-K1 cells were incubated with R24 antibody for 45 min on ice. Afterwards, cells were allowed to internalize the antibody for 20 min at 37°C, and then the temperature was shifted again to 4°C. The cell surface was then stripped of any remaining antibody with acid wash (0 min). Cells were then incubated at 37°C to restore intracellular transport, and cells and culture medium were recovered at 15 and 60 min. The presence of R24 antibody in both samples was analyzed by western blot as indicated in Experimental procedures. (B) The relative contribution of bands in each condition was calculated using the computer software SCION IMAGE on the scanned film shown in (A). Ponceau S staining was used to normalize levels of proteins seeded in each lane. The band intensity for R24 antibody at 0 min (cellular fraction) was arbitrarily taken as 1. Results are representative of four independent experiments.

Ramiro Iglesias-Bartolomé, Crespo, P. M. , Gomez, G. A. , & Daniotti, J. L.. (2006). The antibody to gd3 ganglioside, r24, is rapidly endocytosed and recycled to the plasma membrane via the endocytic recycling compartment : inhibitory effect of brefeldin a and monensin. FEBS Journal, 273(8), 1744-1758.

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Figure 2 R24 antibody is internalized, recycled to the plasma membrane, and released into culture medium in SK-Mel 28 melanoma cells.

Figure 2 R24 antibody is internalized, recycled to the plasma membrane, and released into culture medium in SK-Mel 28 melanoma cells.

(A) SK-Mel 28 melanoma cells were metabolically labeled from [14C]galactose for 24 h. Lipid extracts were prepared, purified, chromatographed and visualized as indicated in Experimental procedures. The positions of cochromatographed glycolipid standards are indicated. The asterisk indicates the position of an unidentified lipid. (B) SK-Mel 28 melanoma cells were incubated with R24 for 45 min at 4°C. After washing of the cells, the temperature was shifted to 37°C to allow endocytosis of the complex GD3–R24, and cells were fixed at 15, 30 and 60 min. R24 antibody was detected by using anti-mouse IgG conjugated with Alexa488. Single confocal sections of 0.7 lm were taken parallel to the coverslip. (C) SK-Mel 28 melanoma cells were incubated with R24 antibody for 45 min on ice. Cells were allowed to internalize the antibody for 20 min at 37°C, and then the temperature was shifted again to 4°C. The cell surface was then stripped of any remaining antibody with acid wash (0 min). Then cells were incubated at 37°C to restore intracellular transport, and cells and culture medium were recovered at 30, 60 and 90 min. The presence of R24 antibody in both samples was analyzed by western blot as indicated in Experimental procedures. Scale bars: 20 μm.

Ramiro Iglesias-Bartolomé, Crespo, P. M. , Gomez, G. A. , & Daniotti, J. L.. (2006). The antibody to gd3 ganglioside, r24, is rapidly endocytosed and recycled to the plasma membrane via the endocytic recycling compartment : inhibitory effect of brefeldin a and monensin. FEBS Journal, 273(8), 1744-1758.


Specifications

  • Immunogen
  • GD3 Ganglioside
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Clone
  • R24
  • Applications
  • ELISA

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody R24 has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Functional Assay. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.

Target

  • Alternative Names
  • GD3 Ganglioside; GD3

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Recombinant Antibody

CAT Product Name Application Type
PABW-049 Human Anti-GD3 Recombinant Antibody (clone chR24) ELISA Chimeric (mouse/human) IgG
FAMAB-0127YC Mouse Anti-GD3 Recombinant Antibody (clone KM641) ELISA, FC, Cyt Mouse IgG3, κ
FAMAB-0128YC Human Anti-GD3 Recombinant Antibody (clone KM871) ELISA, FC, Cyt Chimeric (mouse/human) IgG1, κ
HPAB-0001LY Mouse Anti-GD3 Recombinant Antibody (clone 19B) IHC, TLC immunostaining Mouse IgG

Humanized Antibody

Chimeric Antibody

CAT Product Name Application Type
TAB-082CQ Human Anti-GD3 Recombinant Antibody (TAB-082CQ) IF, ELISA Chimeric antibody (mouse/human)
TAB-082CQ-S(P) Mouse Anti-GD3 Recombinant Antibody; scFv Fragment (TAB-082CQ-S(P)) IF, ELISA Mouse scFv
TAB-082CQ-F(E) Human Anti-GD3 Recombinant Antibody; Fab Fragment (TAB-082CQ-F(E)) IF, ELISA Chimeric antibody (mouse/human)

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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