Recombinant Anti-GFP VHH Single Domain Antibody (GBP1)

Figure 1 Additional information on antigen-specific effectors.

Plasmids encoding unmodified or destabilized GBP1 fusion to Cre or Flpo were transfected into 293T cells along with CAG-driven, loxP-Neo-loxP- (LNL-) or FRT-Neo-FRT- (FNF-) DsRed. Cre and Flpo were each individually fused to either GBP1, dGBP1-GBP1, or dGBP1x2, respectively (see cartoon). Compared to dGBP1-GBP1 fusion, dGBP1x2 gave increased suppression of background recombinase activity. O/E: overexposed. Cells imaged at 22 h post-transfection.

Tang, J. C., Drokhlyansky, E., Etemad, B., Rudolph, S., Guo, B., Wang, S., ... & Cepko, C. L. (2016). Detection and manipulation of live antigen-expressing cells using conditionally stable nanobodies. Elife, 5, e15312.

Figure 2 Additional information on antigen-specific effectors.

Plasmids encoding unmodified or destabilized GBP1 fusion to Cre or Flpo were transfected into 293T cells along with CAG-driven, loxP-Neo-loxP- (LNL-) or FRT-Neo-FRT- (FNF-) DsRed. Cre and Flpo were each individually fused to either GBP1, dGBP1-GBP1, or dGBP1x2, respectively (see cartoon). Compared to dGBP1-GBP1 fusion, dGBP1x2 gave increased suppression of background recombinase activity. O/E: overexposed. Cells imaged at 50 h post-transfection.

Tang, J. C., Drokhlyansky, E., Etemad, B., Rudolph, S., Guo, B., Wang, S., ... & Cepko, C. L. (2016). Detection and manipulation of live antigen-expressing cells using conditionally stable nanobodies. Elife, 5, e15312.