Recombinant Anti-GFP VHH Single Domain Antibody (GBP1) (CAT#: PNBL-064)

Anti-GFP VHH Single Domain Antibody is a recombinant protein produced in E. coli.

  • Published Data
  • Datasheet
  • MSDS
  • COA

Figure 1 Additional information on antigen-specific effectors.

Plasmids encoding unmodified or destabilized GBP1 fusion to Cre or Flpo were transfected into 293T cells along with CAG-driven, loxP-Neo-loxP- (LNL-) or FRT-Neo-FRT- (FNF-) DsRed. Cre and Flpo were each individually fused to either GBP1, dGBP1-GBP1, or dGBP1x2, respectively (see cartoon). Compared to dGBP1-GBP1 fusion, dGBP1x2 gave increased suppression of background recombinase activity. O/E: overexposed. Cells imaged at 22 h post-transfection.

Tang, J. C., Drokhlyansky, E., Etemad, B., Rudolph, S., Guo, B., Wang, S., ... & Cepko, C. L. (2016). Detection and manipulation of live antigen-expressing cells using conditionally stable nanobodies. Elife, 5, e15312.

Figure 2 Additional information on antigen-specific effectors.

Plasmids encoding unmodified or destabilized GBP1 fusion to Cre or Flpo were transfected into 293T cells along with CAG-driven, loxP-Neo-loxP- (LNL-) or FRT-Neo-FRT- (FNF-) DsRed. Cre and Flpo were each individually fused to either GBP1, dGBP1-GBP1, or dGBP1x2, respectively (see cartoon). Compared to dGBP1-GBP1 fusion, dGBP1x2 gave increased suppression of background recombinase activity. O/E: overexposed. Cells imaged at 50 h post-transfection.

Tang, J. C., Drokhlyansky, E., Etemad, B., Rudolph, S., Guo, B., Wang, S., ... & Cepko, C. L. (2016). Detection and manipulation of live antigen-expressing cells using conditionally stable nanobodies. Elife, 5, e15312.


Specifications

  • Host Species
  • Llama
  • Derivation
  • Llama
  • Type
  • Llama VHH
  • Species Reactivity
  • Aequorea victoria
  • Clone
  • GBP1

Target

  • Alternative Names
  • GFP; green fluorescent protein;

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "GFP"

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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