Recombinant Anti-GFP VHH Single Domain Antibody (GBP1) (CAT#: PNBL-064)

Anti-GFP VHH Single Domain Antibody is a recombinant protein produced in E. coli.


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Figure 1 Additional information on antigen-specific effectors.

Figure 1 Additional information on antigen-specific effectors.

Plasmids encoding unmodified or destabilized GBP1 fusion to Cre or Flpo were transfected into 293T cells along with CAG-driven, loxP-Neo-loxP- (LNL-) or FRT-Neo-FRT- (FNF-) DsRed. Cre and Flpo were each individually fused to either GBP1, dGBP1-GBP1, or dGBP1x2, respectively (see cartoon). Compared to dGBP1-GBP1 fusion, dGBP1x2 gave increased suppression of background recombinase activity. O/E: overexposed. Cells imaged at 22 h post-transfection.

Tang, J. C., Drokhlyansky, E., Etemad, B., Rudolph, S., Guo, B., Wang, S., ... & Cepko, C. L. (2016). Detection and manipulation of live antigen-expressing cells using conditionally stable sdAbs. Elife, 5, e15312.

Figure 2 Additional information on antigen-specific effectors.

Figure 2 Additional information on antigen-specific effectors.

Plasmids encoding unmodified or destabilized GBP1 fusion to Cre or Flpo were transfected into 293T cells along with CAG-driven, loxP-Neo-loxP- (LNL-) or FRT-Neo-FRT- (FNF-) DsRed. Cre and Flpo were each individually fused to either GBP1, dGBP1-GBP1, or dGBP1x2, respectively (see cartoon). Compared to dGBP1-GBP1 fusion, dGBP1x2 gave increased suppression of background recombinase activity. O/E: overexposed. Cells imaged at 50 h post-transfection.

Tang, J. C., Drokhlyansky, E., Etemad, B., Rudolph, S., Guo, B., Wang, S., ... & Cepko, C. L. (2016). Detection and manipulation of live antigen-expressing cells using conditionally stable sdAbs. Elife, 5, e15312.


Specifications

  • Host Species
  • Llama
  • Derivation
  • Llama
  • Type
  • Llama VHH
  • Species Reactivity
  • Aequorea victoria
  • Clone
  • GBP1

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Target

  • Alternative Names
  • GFP; green fluorescent protein;

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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CAT Product Name Application Type
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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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