Hi-Affi™ Rabbit Anti-GJA1 Recombinant Antibody (clone DS1445AB)
CAT#: MOR-1445
Recombinant Rabbit anti-GJA1 monoclonal antibody for ELISA, ICC/IF, IHC-P, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line AF22
Immunofluorescent staining of human cell line AF22 shows localization to cell junctions & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47551/1786_D4_6_selected.jpg
Normal Tissue
Figure 2 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10753/26414_A_6_6.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10753/26414_B_8_5.jpg
Normal Tissue
Figure 4 Parathyroid gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10753/26414_A_2_8.jpg
Normal Tissue
Figure 5 Colon
Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10753/26414_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10753/26414_A_8_5.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (hTERT-RPE1, HUVEC TERT2, NTERA-2, SuSa, U-87 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000152661-GJA1
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Specifications
- Immunogen
- Synthetic non-phosphopeptide derived from human Connexin 43/GJA1 around the phosphorylation site of serine 261 (C-G-SP-Q-K).
- Host Species
- Rabbit
- Derivation
- Phage display library
- Type
- Rabbit IgG
- Specificity
- Human GJA1
- Species Reactivity
- Human
- Clone
- DS1445AB
- Applications
- ELISA, ICC, IF, IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Gap Junction Protein Alpha 1; Gap Junction Protein, Alpha 1, 43kDa; Gap Junction 43 KDa Heart Protein; Connexin 43; Connexin-43; CX43; GJAL; Gap Junction Protein, Alpha 1, 43kDa (Connexin 43); Oculodentodigital Dysplasia (Syndactyly Type III); Gap Junction Protein, Alpha-Like; AVSD3; HLHS1; PPKCA; CMDR; EKVP; ODDD; HSS
- Gene ID
- 2697
- UniProt ID
- P17302
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "GJA1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2247z | Mouse Anti-GJA1 Recombinant Antibody (clone 38A10) | FC | Mouse IgG2a |
| MOB-2457CT | Recombinant Mouse anti-Human GJA1 Monoclonal antibody (3E5) | ELISA, WB | |
| MRO-1784-CN | Rabbit Anti-GJA1 Polyclonal Antibody (MRO-1784-CN) | WB, IHC, FC | Rabbit IgG |
| HPAB-M0496-YC | Mouse Anti-GJA1 Recombinant Antibody (HPAB-M0496-YC) | IHC, Inhib, FuncS | Mouse IgG |
| HPAB-M0497-YC | Mouse Anti-GJA1 Recombinant Antibody (HPAB-M0497-YC) | IHC, Inhib, FuncS | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0496-YC-S(P) | Mouse Anti-GJA1 Recombinant Antibody; scFv Fragment (HPAB-M0496-YC-S(P)) | IHC, Inhib, FuncS | Mouse scFv |
| HPAB-M0497-YC-S(P) | Mouse Anti-GJA1 Recombinant Antibody; scFv Fragment (HPAB-M0497-YC-S(P)) | IHC, Inhib, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0496-YC-F(E) | Mouse Anti-GJA1 Recombinant Antibody; Fab Fragment (HPAB-M0496-YC-F(E)) | IHC, Inhib, FuncS | Mouse Fab |
| HPAB-M0497-YC-F(E) | Mouse Anti-GJA1 Recombinant Antibody; Fab Fragment (HPAB-M0497-YC-F(E)) | IHC, Inhib, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0088-FY | Rabbit Anti-GJA1 Recombinant Antibody (clone AFY0059) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY918 | Anti-GJA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2811 | Anti-Mouse GJA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2810 | Anti-Human GJA1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC474 | Recombinant Anti-GJA1 Vesicular Antibody, EV Displayed (VS-0425-YC474) | ELISA, FC, Inhib, Cell-uptake |
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