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  • Hi-Affi™ Rabbit Anti-GLI3 Recombinant Antibody (clone DS1452AB)

Hi-Affi™ Rabbit Anti-GLI3 Recombinant Antibody (clone DS1452AB) (CAT#: MOR-1452)

Recombinant Rabbit anti-GLI3 monoclonal antibody for ICC/IF, WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.


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  • Purity:
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  • Gene Expression
  • Datasheet
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  • COA
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/61_A4_2_selected.jpg
Figure 3 IF staining of human cell line HeLa
Figure 3 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/1975_H4_2_red_green.jpg
Figure 4 IF staining of human cell line U-2 OS
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/59_A4_1_red_green.jpg
Normal Tissue
Figure 2 IHC staining of human testis
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/16128_A_6_6_selected.jpg
Figure 5 Cerebral cortex
Figure 5 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/16128_B_7_5.jpg
Figure 6 Testis
Figure 6 Testis
(Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/16128_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (AF22, WM-115)
Figure 7 RNA cell line category: Cell line enhanced (AF22, WM-115)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000106571-GLI3

Specifications

  • Immunogen
  • Recombinant fragment within Human Gli3 aa 1300-1500.
  • Host Species
  • Rabbit
  • Derivation
  • Phage display library
  • Type
  • Rabbit IgG
  • Specificity
  • Human GLI3
  • Species Reactivity
  • Human, Predicted to work with: Chimpanzee
  • Clone
  • DS1452AB
  • Applications
  • ICC, IF, WB
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Purified with Absolute Mag™ Protein A Agarose
  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • GLI Family Zinc Finger 3; Glioma-Associated Oncogene Family Zinc Finger 3; GLI-Kruppel Family Member GLI3; Zinc Finger Protein GLI3; Oncogene GLI3; GLI3-190; GLI3FL; Greig Cephalopolysyndactyly Syndrome; GLI3 Full Length Protein; GLI3 Form Of 190 KDa; DNA-Binding Protein; PAP-A; PAPA1; PPDIV; ACLS; PAPA; PAPB; GCPS; PHS

Related Resources

  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "GLI3"

Recombinant Antibody

CAT Product Name Application Type
MOB-396 Recombinant Anti-human GLI3 Antibody WB, IP, FuncS IgG
MHH-396 Recombinant Human Anti-human GLI3 Antibody ELISA, WB, Neut, FuncS IgG
MOB-0773CT Recombinant Mouse anti-Human GLI3 Monoclonal antibody (EML508) WB
MRO-0659-CN Rabbit Anti-GLI3 Recombinant Antibody (clone JG83-39) WB, IF Rabbit IgG

Fab Fragment Antibody

CAT Product Name Application Type
MOB-396-F(E) Recombinant Anti-human GLI3 Antibody Fab Fragment FC, IP, FuncS Fab
MHH-396-F(E) Recombinant Human Anti-human GLI3 Antibody Fab Fragment IF, Neut, FuncS Fab

scFv Fragment Antibody

CAT Product Name Application Type
MOB-396-S(P) Recombinant Anti-human GLI3 Antibody scFv Fragment ELISA, WB, FuncS scFv
MHH-396-S(P) Recombinant Human Anti-human GLI3 Antibody scFv Fragment FC, RIA, Biosensors, FuncS scFv

Intrabody

CAT Product Name Application Type
IAB-B069(A) Recombinant Anti-human GLI3 Intrabody [(D-Arg)9] IF, FC, WB, FuncS vhh-(D-Arg)9
IAB-B069(G) Recombinant Anti-human GLI3 Intrabody [+36 GFP] ICC, Neut, FuncS vhh-(+36GFP)
IAB-B069(T) Recombinant Anti-human GLI3 Intrabody [Tat] ELISA, WB, ICC, FuncS vhh-Tat

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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