Anti-Human GPC3 Single Domain Antibody (TAB-416CQ), Research Grade Biosimilar
CAT#: TAB-416CQ
This antibody binds to a unique conformational epitope in the native form of GPC3 core protein on cancer cells with high affinity. The binding requires both the N-terminus and C-terminus domains of GPC3, and is independent of the HS chains on GPC3.
Published Data
Gene Expression
ELISA
Figure 1 Binding properties of HN3 antibody.
Protein-binding affinity was measured by ELISA using purified GPC3-hFc. (D) Measurement of cell-binding affinity by FACS using the G1 cell line.
Feng, M., Gao, W., Wang, R., Chen, W., Man, Y. G., Figg, W. D., ... & Ho, M. (2013). Therapeutically targeting glypican-3 via a conformation-specific single-domain antibody in hepatocellular carcinoma. Proceedings of the National Academy of Sciences, 110(12), E1083-E1091.
Figure 2 Binding properties of HN3 antibody.
Measurement of cell-binding affinity by FACS using the G1 cell line.
Feng, M., Gao, W., Wang, R., Chen, W., Man, Y. G., Figg, W. D., ... & Ho, M. (2013). Therapeutically targeting glypican-3 via a conformation-specific single-domain antibody in hepatocellular carcinoma. Proceedings of the National Academy of Sciences, 110(12), E1083-E1091.
IP
Figure 3 Binding properties of HN3 antibody.
Immunoprecipitation assay using coexpressed GPC3 N- and C-terminal fragments.
Feng, M., Gao, W., Wang, R., Chen, W., Man, Y. G., Figg, W. D., ... & Ho, M. (2013). Therapeutically targeting glypican-3 via a conformation-specific single-domain antibody in hepatocellular carcinoma. Proceedings of the National Academy of Sciences, 110(12), E1083-E1091.
IHC
Figure 4 Binding properties of HN3 antibody.
HCC tissue immunohistochemistry staining with HN3.
Feng, M., Gao, W., Wang, R., Chen, W., Man, Y. G., Figg, W. D., ... & Ho, M. (2013). Therapeutically targeting glypican-3 via a conformation-specific single-domain antibody in hepatocellular carcinoma. Proceedings of the National Academy of Sciences, 110(12), E1083-E1091.
FuncS
Figure 5 The effect of yap deregulation on HN3 function.
HN3 treatment of yap-knockdown cells. Proliferation was normalized with unengineered cells without antibody treatment.
Feng, M., Gao, W., Wang, R., Chen, W., Man, Y. G., Figg, W. D., ... & Ho, M. (2013). Therapeutically targeting glypican-3 via a conformation-specific single-domain antibody in hepatocellular carcinoma. Proceedings of the National Academy of Sciences, 110(12), E1083-E1091.
FuncS
Figure 6 The effect of yap deregulation on HN3 function.
HN3 treatment of cells overexpressing yap-S127A.
Feng, M., Gao, W., Wang, R., Chen, W., Man, Y. G., Figg, W. D., ... & Ho, M. (2013). Therapeutically targeting glypican-3 via a conformation-specific single-domain antibody in hepatocellular carcinoma. Proceedings of the National Academy of Sciences, 110(12), E1083-E1091.
WB
Figure 7 The effect of yap deregulation on HN3 function.
Western blot analysis of molecular changes after HN3 treatment on yap-knockdown cells or cells overexpressing yap-S127A.
Feng, M., Gao, W., Wang, R., Chen, W., Man, Y. G., Figg, W. D., ... & Ho, M. (2013). Therapeutically targeting glypican-3 via a conformation-specific single-domain antibody in hepatocellular carcinoma. Proceedings of the National Academy of Sciences, 110(12), E1083-E1091.
ELISA
Figure 8 Inhibition of HCC xenograft tumor growth by HN3 antibody.
Pharmacokinetics of HN3 antibody. Serum concentrations of the antibody were measured by ELISA following a single i.v. injection of 3 mg/kg. The serum antibody concentration data were fitted with a two-compartment model using Phoenix WinNonlin software. The observed mean serum antibody concentration (in micrograms per milliliter) ± SD of four animals for each time point was plotted as a function of time.
Feng, M., Gao, W., Wang, R., Chen, W., Man, Y. G., Figg, W. D., ... & Ho, M. (2013). Therapeutically targeting glypican-3 via a conformation-specific single-domain antibody in hepatocellular carcinoma. Proceedings of the National Academy of Sciences, 110(12), E1083-E1091.
Inhib
Figure 9 Inhibition of HCC xenograft tumor growth by HN3 antibody.
Inhibition of HepG2 cells inoculated into nude mice. After the tumor size reached 100 mm³, HN3 antibody or hIgG control was delivered i.v. at 60 mg/kg body weight, twice a week. Arrows indicate antibody injections.
Feng, M., Gao, W., Wang, R., Chen, W., Man, Y. G., Figg, W. D., ... & Ho, M. (2013). Therapeutically targeting glypican-3 via a conformation-specific single-domain antibody in hepatocellular carcinoma. Proceedings of the National Academy of Sciences, 110(12), E1083-E1091.
Inhib
Figure 10 Inhibition of HCC xenograft tumor growth by HN3 antibody.
Inhibition of Huh-7 cells inoculated into nude mice. After the tumor size reached 100 mm³, HN3 antibody or hIgG control was delivered i.v. at 60 mg/kg body weight, twice a week. Arrows indicate antibody injections.
Feng, M., Gao, W., Wang, R., Chen, W., Man, Y. G., Figg, W. D., ... & Ho, M. (2013). Therapeutically targeting glypican-3 via a conformation-specific single-domain antibody in hepatocellular carcinoma. Proceedings of the National Academy of Sciences, 110(12), E1083-E1091.
WB
Figure 11 Inhibition of HCC xenograft tumor growth by HN3 antibody.
Western blot analysis of molecular changes in HN3-treated and control tumors.
Feng, M., Gao, W., Wang, R., Chen, W., Man, Y. G., Figg, W. D., ... & Ho, M. (2013). Therapeutically targeting glypican-3 via a conformation-specific single-domain antibody in hepatocellular carcinoma. Proceedings of the National Academy of Sciences, 110(12), E1083-E1091.
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Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/6316/1404_H7_2_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Group enriched (HAP1, Hep G2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000147257-GPC3
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Specifications
- Host Species
- Human
- Derivation
- Phage display library
- Type
- Single domain antibody
- Specificity
- Human
- Species Reactivity
- Human
- Clone
- TAB-416CQ
- Applications
- Immunoprecipitation, Immunohistochemistry, Functional Assay, Western Blot, ELISA, Inhibition
- Related Disease
- Hepatocellular carcinoma
Applications
- Application Notes
- The antibody was validated for Immunoprecipitation, Immunohistochemistry, Functional Assay, Western Blot, ELISA, Inhibition. For details, refer to Published Data.
Target
REVIEWS AND Q&AS
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Versatile Binder
The Anti-Human GPC3 Single Domain Antibody demonstrated excellent performance in Immunoprecipitation, IHC, and ELISA assays in my research. Its unique binding to a conformational epitope on GPC3 ensured high specificity and affinity. The product information provided clear details of the phage display production process, which helped in experiment optimization.
Consistent Results
I have integrated the single domain antibody for GPC3 into multiple assay systems, including Western Blot and functional tests. The data sheet was very informative regarding critical aspects of the antibody's epitope recognition and expression system. The consistent binding performance across different platforms has improved the reliability of my research findings.
Q&As
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How is the single domain antibody specific for GPC3?
A: We designed the Anti-Human GPC3 Single Domain Antibody using a phage display library to recognize a unique conformational epitope on GPC3. The antibody's specificity and affinity have been confirmed in immunoprecipitation, IHC, and inhibition assays, making it a reliable reagent for studying GPC3 in cancer cell models.
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What should researchers note for using this antibody in multiple assays?
A: We recommend that users optimize experimental conditions using the provided titration guidelines. The single domain antibody has been validated in several assays including Western Blot and ELISA. Its precise binding to both the N- and C-terminus of the GPC3 core protein ensures consistent and reproducible results across various research applications.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-416CQ, RRID: AB_3111929)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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