Recombinant Human Anti-H1N1 HA Antibody (CH65) (CAT#: PABL-482)

Recombinant Human Antibody (CH65) is capable of binding to H1N1 HA, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-H1N1 HA mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-H1N1 HA proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody neutralizes infectivity of 30 out of 36 H1N1 strains tested.


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FuncS

Figure 1 Isolation and characterization of 441D6-like antibodies from human PBMCs.

Figure 1 Isolation and characterization of 441D6-like antibodies from human PBMCs.

Antibody cross-competition profile. Antibody binding to NC99 hemagglutinin was measured by biolayer interferometry in the presence or absence of competing (1°) antibody. Test antibodies are listed as 2º.

Kanekiyo, M., Joyce, M. G., Gillespie, R. A., Gallagher, J. R., Andrews, S. F., Yassine, H. M., ... & Leung, K. (2019). Mosaic nanoparticle display of diverse influenza virus hemagglutinins elicits broad B cell responses. Nature immunology, 20(3), 362-372.

Neut

Figure 2 Breadth, potency and potential mechanism of neutralization of 441D6.

Figure 2 Breadth, potency and potential mechanism of neutralization of 441D6.

Microneutralization activity of 441D6 in its IgG and Fab forms. MN IC50 concentrations of CH65, 441D6 and CR6261 were determined by microneutralization assays with two H1N1 viruses.

Kanekiyo, M., Joyce, M. G., Gillespie, R. A., Gallagher, J. R., Andrews, S. F., Yassine, H. M., ... & Leung, K. (2019). Mosaic nanoparticle display of diverse influenza virus hemagglutinins elicits broad B cell responses. Nature immunology, 20(3), 362-372.

WB

Figure 3 HA head binding antibodies inhibit influenza virus egress.

Figure 3 HA head binding antibodies inhibit influenza virus egress.

Calu-3 cells were infected with A/Puerto Rico/8/1934 (H1N1) and 3 hours later head-binding antibody CH65 was added. Twenty hours later, the amounts of HA present in the cell supernatant (S) and lysate (L) were analyzed by Western blot.

Brandenburg, B., Koudstaal, W., Goudsmit, J., Klaren, V., Tang, C., Bujny, M. V., ... & van Oijen, A. M. (2013). Mechanisms of hemagglutinin targeted influenza virus neutralization. PloS one, 8(12).


Specifications

  • Immunogen
  • H1N1 IAV haemagglutinin
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG
  • Specificity
  • Tested positive against native H1N1 HA
  • Species Reactivity
  • H1N1 IAV
  • Clone
  • CH65
  • Applications
  • FuncS, Neut, WB

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • This antibody has been reported for use in Neutralization, Western Blot and Function Assay.

Target

  • Alternative Names
  • HA; haemagglutinin; HA1; HA2

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Human Antibody

Humanized Antibody

CAT Product Name Application Type
PABX-070-S (P) Recombinant Mouse Anti-HA1 Antibody scFv Fragment (Fab17/9) WB, ELISA, Neut, FuncS scFv

Rabbit Monoclonal Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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