Recombinant Human Anti-H1N1 HA Antibody (CH65) (CAT#: PABL-482)

Figure 1 Isolation and characterization of 441D6-like antibodies from human PBMCs.

Antibody cross-competition profile. Antibody binding to NC99 hemagglutinin was measured by biolayer interferometry in the presence or absence of competing (1°) antibody. Test antibodies are listed as 2º.

Kanekiyo, M., Joyce, M. G., Gillespie, R. A., Gallagher, J. R., Andrews, S. F., Yassine, H. M., ... & Leung, K. (2019). Mosaic nanoparticle display of diverse influenza virus hemagglutinins elicits broad B cell responses. Nature immunology, 20(3), 362-372.

Figure 2 Breadth, potency and potential mechanism of neutralization of 441D6.

Microneutralization activity of 441D6 in its IgG and Fab forms. MN IC50 concentrations of CH65, 441D6 and CR6261 were determined by microneutralization assays with two H1N1 viruses.

Kanekiyo, M., Joyce, M. G., Gillespie, R. A., Gallagher, J. R., Andrews, S. F., Yassine, H. M., ... & Leung, K. (2019). Mosaic nanoparticle display of diverse influenza virus hemagglutinins elicits broad B cell responses. Nature immunology, 20(3), 362-372.

Figure 3 HA head binding antibodies inhibit influenza virus egress.

Calu-3 cells were infected with A/Puerto Rico/8/1934 (H1N1) and 3 hours later head-binding antibody CH65 was added. Twenty hours later, the amounts of HA present in the cell supernatant (S) and lysate (L) were analyzed by Western blot.

Brandenburg, B., Koudstaal, W., Goudsmit, J., Klaren, V., Tang, C., Bujny, M. V., ... & van Oijen, A. M. (2013). Mechanisms of hemagglutinin targeted influenza virus neutralization. PloS one, 8(12).