Recombinant Mouse Anti-H3N2 HA Antibody (S139/1) (CAT#: PABZ-195)

Recombinant Mouse Antibody (S139/1) is capable of binding to H3N2 HA, expressed in Chinese Hamster Ovary cells (CHO). Binding of S139/1 IgG against a panel of 35 HAs within 13 subtypes was assessed by bio-layer interferometry using an Octet RED instrument.

Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Datasheet
  • MSDS
  • COA

Figure 1 Binding and neutralization breadth of influenza HA strains by S139/1 Fab and IgG

Figure 1 Binding and neutralization breadth of influenza HA strains by S139/1 Fab and IgG

Lee, P. S., Yoshida, R., Ekiert, D. C., Sakai, N., Suzuki, Y., Takada, A., & Wilson, I. A. (2012). Heterosubtypic antibody recognition of the influenza virus hemagglutinin receptor binding site enhanced by avidity. Proceedings of the National Academy of Sciences, 201212371.

Figure 2 HA sequence variation at the S139/1 epitope

Figure 2 HA sequence variation at the S139/1 epitope

Lee, P. S., Yoshida, R., Ekiert, D. C., Sakai, N., Suzuki, Y., Takada, A., & Wilson, I. A. (2012). Heterosubtypic antibody recognition of the influenza virus hemagglutinin receptor binding site enhanced by avidity. Proceedings of the National Academy of Sciences, 201212371.

Figure 3 Reactivity of MAb S139/1 to various influenza virus strains.

Figure 3 Reactivity of MAb S139/1 to various influenza virus strains.

Binding activity of MAb S139/1 at the concentrations of 100 µg/ml (grey), 10 µg/ml (white), and 1 µg/ml (black) to the indicated virus strains was measured by ELISA.

Yoshida, R., Igarashi, M., Ozaki, H., Kishida, N., Tomabechi, D., Kida, H., ... & Takada, A. (2009). Cross-protective potential of a novel monoclonal antibody directed against antigenic site B of the hemagglutinin of influenza A viruses. PLoS pathogens, 5(3), e1000350.

Figure 4 Neutralization activity of MAb S139/1 to various HA subtypes of influenza A virus strains.

Figure 4 Neutralization activity of MAb S139/1 to various HA subtypes of influenza A virus strains.

Viruses were mixed with indicated concentrations of the purified MAb S139/1 (A) or control IgG2a (ZGP12/1.1) (B). Neutralization activities were evaluated by plaque reduction assays using MDCK cells.

Yoshida, R., Igarashi, M., Ozaki, H., Kishida, N., Tomabechi, D., Kida, H., ... & Takada, A. (2009). Cross-protective potential of a novel monoclonal antibody directed against antigenic site B of the hemagglutinin of influenza A viruses. PLoS pathogens, 5(3), e1000350.

Figure 5 HI activity of MAb S139/1 with various influenza virus strains.

Figure 5 HI activity of MAb S139/1 with various influenza virus strains.

Yoshida, R., Igarashi, M., Ozaki, H., Kishida, N., Tomabechi, D., Kida, H., ... & Takada, A. (2009). Cross-protective potential of a novel monoclonal antibody directed against antigenic site B of the hemagglutinin of influenza A viruses. PLoS pathogens, 5(3), e1000350.

Figure 6 Protective efficacy of passive immunization with MAb S139/1 in mice.

Figure 6 Protective efficacy of passive immunization with MAb S139/1 in mice.

Mice were passively immunized before (A) or after (B) virus challenge with Aichi (H3) or WSN (H1). Control mice were given MAb ZGP12/1.1 or PBS. Virus titers of the lung were determined by a plaque assay. The means and standard deviations are shown. For statistical purposes, samples with undetectable virus titers were given the value 2.0 log₁₀ PFU/g. The data of pre-immunized mice were analyzed using the nonparametric Kruskal-Wallis ANOVA on ranks, followed by the Mann-Whitney U-test with the Bonferroni correction for multiple comparisons. The data of post-immunized mice were analyzed using the Mann-Whitney U-test. Two-sided p values less than 0.05 were considered statistically significant. Significant differences were indicated by asterisks (*** p<0.001, ** p<0.01, * p<0.05). All statistical analyses were performed with the computer program R (version 2.8.1).

Yoshida, R., Igarashi, M., Ozaki, H., Kishida, N., Tomabechi, D., Kida, H., ... & Takada, A. (2009). Cross-protective potential of a novel monoclonal antibody directed against antigenic site B of the hemagglutinin of influenza A viruses. PLoS pathogens, 5(3), e1000350.


Specifications

  • Immunogen
  • IAV Hemagglutinin
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • IgG
  • Specificity
  • Tested positive against native H3N2 HA
  • Species Reactivity
  • IAV
  • Clone
  • S139/1
  • Applications
  • Neut 0-100 μg/mL
    ELISA 1-100 μg/mL
    FuncS Assay-Dependent

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • The antibody S139/1 has been reported in applications of Neut, ELISA, FuncS. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
    Neut: A reported concentration of the antibody S139/1 for neutralization was 0-100 μg/mL.
    ELISA: A reported concentration of the antibody S139/1 for ELISA was 0-100 μg/mL.

Target

  • Alternative Names
  • HA; Hemagglutinin

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone S139/1"

See other products for "HA"

Humanized Antibody

CAT Product Name Application Type
PABX-070-S (P) Recombinant Mouse Anti-HA1 Antibody scFv Fragment (Fab17/9) WB, ELISA, Neut, FuncS scFv

Rabbit Monoclonal Antibody

Submit a review or a question
There are currently no Customer reviews or questions for PABZ-195. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart

    Go to compare