Recombinant Mouse Anti-H3N2 HA Antibody (S139/1)

Lee, P. S., Yoshida, R., Ekiert, D. C., Sakai, N., Suzuki, Y., Takada, A., & Wilson, I. A. (2012). Heterosubtypic antibody recognition of the influenza virus hemagglutinin receptor binding site enhanced by avidity. Proceedings of the National Academy of Sciences, 201212371.

Lee, P. S., Yoshida, R., Ekiert, D. C., Sakai, N., Suzuki, Y., Takada, A., & Wilson, I. A. (2012). Heterosubtypic antibody recognition of the influenza virus hemagglutinin receptor binding site enhanced by avidity. Proceedings of the National Academy of Sciences, 201212371.

Binding activity of MAb S139/1 at the concentrations of 100 µg/ml (grey), 10 µg/ml (white), and 1 µg/ml (black) to the indicated virus strains was measured by ELISA.

Yoshida, R., Igarashi, M., Ozaki, H., Kishida, N., Tomabechi, D., Kida, H., ... & Takada, A. (2009). Cross-protective potential of a novel monoclonal antibody directed against antigenic site B of the hemagglutinin of influenza A viruses. PLoS pathogens, 5(3), e1000350.

Viruses were mixed with indicated concentrations of the purified MAb S139/1 (A) or control IgG2a (ZGP12/1.1) (B). Neutralization activities were evaluated by plaque reduction assays using MDCK cells.

Yoshida, R., Igarashi, M., Ozaki, H., Kishida, N., Tomabechi, D., Kida, H., ... & Takada, A. (2009). Cross-protective potential of a novel monoclonal antibody directed against antigenic site B of the hemagglutinin of influenza A viruses. PLoS pathogens, 5(3), e1000350.

Yoshida, R., Igarashi, M., Ozaki, H., Kishida, N., Tomabechi, D., Kida, H., ... & Takada, A. (2009). Cross-protective potential of a novel monoclonal antibody directed against antigenic site B of the hemagglutinin of influenza A viruses. PLoS pathogens, 5(3), e1000350.

Mice were passively immunized before (A) or after (B) virus challenge with Aichi (H3) or WSN (H1). Control mice were given MAb ZGP12/1.1 or PBS. Virus titers of the lung were determined by a plaque assay. The means and standard deviations are shown. For statistical purposes, samples with undetectable virus titers were given the value 2.0 log₁₀ PFU/g. The data of pre-immunized mice were analyzed using the nonparametric Kruskal-Wallis ANOVA on ranks, followed by the Mann-Whitney U-test with the Bonferroni correction for multiple comparisons. The data of post-immunized mice were analyzed using the Mann-Whitney U-test. Two-sided p values less than 0.05 were considered statistically significant. Significant differences were indicated by asterisks (*** p<0.001, ** p<0.01, * p<0.05). All statistical analyses were performed with the computer program R (version 2.8.1).

Yoshida, R., Igarashi, M., Ozaki, H., Kishida, N., Tomabechi, D., Kida, H., ... & Takada, A. (2009). Cross-protective potential of a novel monoclonal antibody directed against antigenic site B of the hemagglutinin of influenza A viruses. PLoS pathogens, 5(3), e1000350.