Recombinant Human Anti-HCV E2 Antibody (MRCT10.v362) (CAT#: PABL-124)

Figure 2 (a) Ratio of dissociation constants (KD) for MRCT10.v362 (red) and hu5B3.v3 (black) binding to alanine mutant peptides relative to WT peptide QLINTNGSWHINGSGK-biotin (E2412–423-biotin) are graphed. Asterisks denote no binding detected with mutant peptides.

Pantua, H., Diao, J., Ultsch, M., Hazen, M., Mathieu, M., McCutcheon, K.,... & Hass, P. (2013). Glycan shifting on hepatitis C virus (HCV) E2 glycoprotein is a mechanism for escape from broadly neutralizing antibodies. Journal of molecular biology, 425(11), 1899-1914.

Figure 3 (b) Binding of various E2412–423-specific neutralizing antibodies to lysates of untransfected 293 cells (No E2) or 293 cells transfected with genotype 2a (J6CF) HCV E1E2-expressing plasmids containing various mutations at N415 or N417.

Pantua, H., Diao, J., Ultsch, M., Hazen, M., Mathieu, M., McCutcheon, K.,... & Hass, P. (2013). Glycan shifting on hepatitis C virus (HCV) E2 glycoprotein is a mechanism for escape from broadly neutralizing antibodies. Journal of molecular biology, 425(11), 1899-1914.

Figure 4 (c and d) MRCT10 Fab binds to N417S E2412–423. Biacore sensorgrams demonstrating binding of MRCT10 Fab to WT (c) or N417S (d) E2412–423 peptide. The Fab series was diluted 2-fold starting from 1000 nM down to 15.6 nM. MRCT10 Fab binds to N417S E2412–423 peptide with faster off-rate compared to WT. Dissociation constants (KD) are listed in the graphs.

Pantua, H., Diao, J., Ultsch, M., Hazen, M., Mathieu, M., McCutcheon, K.,... & Hass, P. (2013). Glycan shifting on hepatitis C virus (HCV) E2 glycoprotein is a mechanism for escape from broadly neutralizing antibodies. Journal of molecular biology, 425(11), 1899-1914.

Figure 5 Enhancement of antiviral effect of NS3 protease inhibitors by inhibition of HCV entry. DMSO-differentiated Huh7.5 cells were infected with Jc1 HCVcc (MOI = 0.05) alone (filled squares) or in the presence of MRCT10.v362 (10 μg/mL, open circles), Telaprevir (2 μM, open triangles) or a combination of MRCT10.v362 and Telaprevir (open diamonds). HCV RNA copies were measured at various times post infection. HCV cDNA derived from the day 22 post infection cultures was sequenced (denoted by the asterisk).

Pantua, H., Diao, J., Ultsch, M., Hazen, M., Mathieu, M., McCutcheon, K.,... & Hass, P. (2013). Glycan shifting on hepatitis C virus (HCV) E2 glycoprotein is a mechanism for escape from broadly neutralizing antibodies. Journal of molecular biology, 425(11), 1899-1914.