Anti-HER2/neu Antibody-Cytokine Fusion Protein, IgG (trastuzumab)-GM-CSF (CAT#: ACFP-SH063)
This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-HER2/neu IgG to GM-CSF. It was expressed in CHO and purified with affinity chromatography. The immunocytokine retains the ability to bind the HER2/neu as well as the biological activity of GM-CSF. In addition, the anti-HER2/neu IgG3-(GM-CSF) protein was capable of activating J774.2 murine macrophage cells enhancing their direct cytotoxic activity and also enhancing antibody-dependent macrophage-mediated tumor cell lysis. The anti-HER2/neu IgG3-(GM-CSF) fusion protein will be useful in the treatment of human HER2/neu-expressing tumors. This immunocytokine was designed for treating HER-2/neu over-expressing cancer.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1060/169_D12_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1060/169_D12_1_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1338/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1338/if_selected.jpg
Figure 3 High expression in esophagus
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1383/4682_B_5_1_val_selected.jpg
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1383/4682_B_5_1_val_selected.jpg
Figure 4 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/ihc_selected.jpg
(Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/ihc_selected.jpg
Figure 5 IHC staining of human urinary bladder
(Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/ihc_selected.jpg
(Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/ihc_selected.jpg
Figure 6 Kidney
(Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/1054_A_7_5.jpg
(Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/1054_A_7_5.jpg
Figure 7 Breast
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/45831_B_3_4.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/45831_B_3_4.jpg
Figure 8 RNA cell line category: Group enriched (OE19, SK-BR-3)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141736-ERBB2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141736-ERBB2
Specifications
- Target
- HER2/neu
- Molecule Class
- L Kappa; IgG1-GM-CSF
- Species Reactivity
- Human
- Expression Host
- CHO
- Clone
- trastuzumab
- Related Disease
- HER-2/neu over-expressing cancer
- Cytokine 1
- GM-CSF
- Cytokines 1 Description
- Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim.
- Cytokine 1 Species
- Human
- Applications
- This product can be used therapeutically to deliver biologically active ligands to a desired tissue.
Target
- Alternative Names
- ERBB2; erb-b2 receptor tyrosine kinase 2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu; receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; human epidermal growth factor receptor 2; neuro/glioblastoma derived oncogene homolog; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2; neuro/glioblastoma derived oncogene homolog
- Gene ID
- 2064
- UniProt ID
- P04626
Related Resources
Bladder Cancer
Non-small Cell Lung Cancer
ErbB Signaling Pathway
Downloads
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Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-059 | Recombinant Anti-Human ERBB2 VHH Single Domain Antibody | IHC, FC, CA, FuncS | Llama VHH |
| PNBL-066 | Recombinant Anti-HER2 VHH Single Domain Antibody (Gr3) | SPR | Llama VHH |
| HPAB-0201-FY | Recombinant llama Anti-ERBB2 Antibody (VHH6) | E | llama VHH |
| HPAB-0202-FY | Recombinant llama Anti-ERBB2 Antibody (VHH1) | E | llama VHH |
| HPAB-0203-FY | Recombinant llama Anti-ERBB2 Antibody | IF | llama VHH |
Intrabody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B008(A) | Recombinant Anti-human ERBB2 Intrabody [(D-Arg)9] | IF, FC, FuncS | scFv-(D-Arg)9 |
| IAB-B008(G) | Recombinant Anti-human ERBB2 Intrabody [+36 GFP] | WB, ICC, FuncS | scFv-(+36GFP) |
| IAB-B008(T) | Recombinant Anti-human ERBB2 Intrabody [Tat] | ICC, Neut, FuncS | scFv-Tat |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-761 | Anti-ERBB2 Recombinant Antibody (Margetuximab) | IF, IP, Neut, FuncS, ELISA, FC, WB | IgG1 - kappa |
| TAB-049CT | Anti-Human HER2/neu Recombinant Antibody (ChA21) | IHC, Inhib, WB, Activ, FC | Chimeric antibody (mouse/human) |
| TAB-049CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (ChA21) | WB | Chimeric antibody (mouse/human) |
| TAB-049CT-F(E) | Anti-Human HER2/neu Recombinant Antibody Fab Fragment (ChA21) | WB | Chimeric antibody (mouse/human) |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G022E | Anti-ERBB2 immunotoxin 4D5 (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-G022D | Anti-ERBB2 immunotoxin 4D5 (scFv)-DT | Cytotoxicity assay, Function study | |
| AGTO-G022G | Anti-ERBB2 immunotoxin 4D5 (scFv)-Gel | Cytotoxicity assay, Function study | |
| AGTO-G022R | Anti-ERBB2 immunotoxin 4D5 (scFv)-RTA | Cytotoxicity assay, Function study | |
| AGTO-G022S | Anti-ERBB2 immunotoxin 4D5 (scFv)-Sap | Cytotoxicity assay, Function study |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-463 | Human Anti-ERBB2 Recombinant Antibody (clone mAb37); scFv Fragment | WB | Human scFv |
| HPAB-S0027-YC-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone F5); scFv Fragment | ELISA, Inhib, Internalization | Human scFv |
| HPAB-S0028-YC-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone C1); scFv Fragment | ELISA, Inhib, Internalization | Human scFv |
| HPAB-1855-FY-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-1855-FY-F(E)) | ELISA | Humanized Fab |
| PSBS-0070 | Human Anti-ERBB2 Recombinant Antibody; scFv Fragment (PSBS-0070) | ELISA, FuncS | Human scFv |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-050CT | Anti-Human HER2/neu Recombinant Antibody (HuA21) | Inhib, Cty, IHC, WB, IF, FC, ELISA | Humanized antibody |
| TAB-053CT | Human Anti-ERBB2 Recombinant Antibody (TAB-053CT) | ELISA, Inhib, FC | Humanized IgG |
| TAB-050CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (HuA21) | ELISA, WB | Humanized antibody |
| TAB-053CT-S(P) | Human Anti-ERBB2 Recombinant Antibody; scFv Fragment (TAB-053CT-S(P)) | ELISA, Inhib, FC | Humanized scFv |
| TAB-468CQ | Anti-Human ERBB2 Recombinant Antibody (Timigutuzumab) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, κ |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-055CT | Anti-Human HER2/neu Recombinant Antibody | WB | Human antibody |
| TAB-056CT | Human Anti-ERBB2 Recombinant Antibody (TAB-056CT) | WB | Human IgG |
| TAB-055CT-F(E) | Anti-Human HER2/neu Recombinant Antibody Fab Fragment | WB | Human antibody |
| TAB-056CT-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (TAB-056CT-F(E)) | WB | Human Fab |
| PABX-081-S (P) | Recombinant Human Anti-HER2 Antibody scFv Fragment (Gr3) | WB, ELISA, FuncS | scFv |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-034CT-F(E) | Anti-Human HER2/neu Recombinant Antibody Fab Fragment (pertuzumab ) | WB | |
| TAB-036CT-F(E) | Anti-Human HER2/neu Recombinant Antibody Fab Fragment (6B2) | ELISA, WB | |
| TAB-037CT-F(E) | Anti-Human HER2/neu Recombinant Antibody Fab Fragment (7C2/7F3) | ELISA, WB | |
| TAB-270CQ | Mouse Anti-ERBB2 Recombinant Antibody (TAB-270CQ) | WB, Inhib | Mouse IgG |
| PABX-082-S (P) | Recombinant Human Anti-HER2 Antibody scFv Fragment (Gr6) | WB, ELISA, FuncS | scFv |
Fab Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-024LC | Recombinant Anti-Human ERBB2 Antibody (Fab glycosylation) | ELISA | Mouse antibody |
| Gly-039LC | Recombinant Anti-Human ERBB2 Antibody (Fab glycosylation) | ELISA | Human antibody |
Fc Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-117LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Humanized antibody |
| Gly-118LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Mouse antibody |
| Gly-145LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
| Gly-146LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
| Gly-147LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
Deglycosylated Antibody (Non-glycosylated IgGs)
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-177LC | Recombinant Anti-Human ERBB2 Antibody (Non-glycosylated) | ELISA | Humanized antibody |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0103MZ | Recombinant Mouse Anti-ERBB2 Antibody (clone C980M) | ELISA | Mouse antibody |
| HPAB-M0510-YC | Mouse Anti-ERBB2 Recombinant Antibody (HPAB-M0510-YC) | FC, FuncS | Mouse IgG2a |
| HPAB-1129-FY | Human Anti-ERBB2 Recombinant Antibody (clone Her2-S1R2A-CS-1F7) | ELISA, WB, FC, IF, IP | Human IgG |
| HPAB-1130-FY | Human Anti-ERBB2 Recombinant Antibody (clone Her2-S1R2A-CS-1D11) | ELISA, WB, FC, IF, IP | Human IgG |
| HPAB-1131-FY | Human Anti-ERBB2 Recombinant Antibody (clone Her2-S1R2C-CS-1D3) | ELISA, WB, FC, IF, IP | Human IgG |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0195MZ | Chicken Anti-ERBB2 Polyclonal IgY | WB | Chicken antibody |
| BRD-0695MZ | Chicken Anti-ERBB2 Polyclonal IgY | WB | Chicken antibody |
| BRD-0779MZ | Chicken Anti-ErbB2 Polyclonal IgY | ICC, IF, IHC, IP | Chicken antibody |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-737CQ | Mouse Anti-ERBB2 Recombinant Antibody (clone 7.16.4) | Block, IP, IF, FC | Mouse IgG2a |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1178 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone DS1178AB) | IHC-P, IHC-Fr | Rabbit IgG |
| MOR-4586 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone TH99DS) | WB | Rabbit IgG |
| MOR-4587 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone TH100DS) | WB | Rabbit IgG |
MHC Tetramer for Cancer
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0010-FY-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-0010-FY-F(E)) | ELISA, FC | Human Fab |
| HPAB-0011-FY-F(E) | Mouse Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-0011-FY-F(E)) | Inhib | Mouse Fab |
| HPAB-0608LY-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-0608LY-F(E)) | ELISA, Inhib | Humanized Fab |
| HPAB-0609LY-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-0609LY-F(E)) | ELISA, Inhib | Humanized Fab |
| HPAB-0610LY-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-0610LY-F(E)) | ELISA, Inhib | Humanized Fab |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-468CQ | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Timigutuzumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| AFC-TAB-053 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Pertuzumab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
| AFC-TAB-761 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Margetuximab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-005 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Trastuzumab), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
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