Anti-HER2/neu Antibody-Cytokine Fusion Protein, scFv‑Fc (trastuzumab)-IL2 (CAT#: ACFP-SH153)
This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-HER2/neu scFv‑Fc to IL2. It was expressed in CHO and purified with affinity chromatography. The immunocytokine retains the ability to bind the HER2/neu as well as the biological activity of IL2. The anti‑erbB2 scFv‑Fc‑IL‑2 fusion protein (HFI) has improved ability to mediate ADCC activity in vitro., IL‑2 was recruited to target cells to block HER2 signaling, inhibit or kill tumor cells, improve the immune capacity, reduce the dose of antibody and IL‑2 synergy. This immunocytokine was designed for treating HER-3/neu over-expressing cancer.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1060/169_D12_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1060/169_D12_1_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1338/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1338/if_selected.jpg
Figure 3 High expression in esophagus
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1383/4682_B_5_1_val_selected.jpg
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1383/4682_B_5_1_val_selected.jpg
Figure 4 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/ihc_selected.jpg
(Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/ihc_selected.jpg
Figure 5 IHC staining of human urinary bladder
(Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/ihc_selected.jpg
(Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/ihc_selected.jpg
Figure 6 Kidney
(Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/1054_A_7_5.jpg
(Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/1054_A_7_5.jpg
Figure 7 Breast
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/45831_B_3_4.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/45831_B_3_4.jpg
Figure 8 RNA cell line category: Group enriched (OE19, SK-BR-3)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141736-ERBB2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141736-ERBB2
Specifications
- Target
- HER2/neu
- Molecule Class
- scFv‑Fc‑IL2
- Species Reactivity
- Human
- Expression Host
- CHO
- Clone
- trastuzumab
- Related Disease
- HER-3/neu over-expressing cancer
- Cytokine 1
- IL2
- Cytokines 1 Description
- Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 is part of the body's natural response to microbial infection, and in discriminating between foreign ("non-self") and "self". IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes.
- Cytokine 1 Species
- Human
- Applications
- This product can be used therapeutically to deliver biologically active ligands to a desired tissue.
Target
- Alternative Names
- ERBB2; erb-b2 receptor tyrosine kinase 2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu; receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; human epidermal growth factor receptor 2; neuro/glioblastoma derived oncogene homolog; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2; neuro/glioblastoma derived oncogene homolog
- Gene ID
- 2064
- UniProt ID
- P04626
Related Resources
Bladder Cancer
Non-small Cell Lung Cancer
ErbB Signaling Pathway
Downloads
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Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-059 | Recombinant Anti-Human ERBB2 VHH Single Domain Antibody | IHC, FC, CA, FuncS | Llama VHH |
| PNBL-066 | Recombinant Anti-HER2 VHH Single Domain Antibody (Gr3) | SPR | Llama VHH |
| PNBL-067 | Recombinant Anti-HER2 VHH Single Domain Antibody (Gr6) | SPR | Llama VHH |
| HPAB-1026WJ | Recombinant Camelid Anti-ERBB2 Single Domain Antibody | ELISA | Camelid VHH |
| HPAB-0725-YJ-VHH | Camelid Anti-ERBB2 Recombinant Single Domain Antibody (SR-87) | ELISA | Camelid VHH |
Intrabody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B008(A) | Recombinant Anti-human ERBB2 Intrabody [(D-Arg)9] | IF, FC, FuncS | scFv-(D-Arg)9 |
| IAB-B008(G) | Recombinant Anti-human ERBB2 Intrabody [+36 GFP] | WB, ICC, FuncS | scFv-(+36GFP) |
| IAB-B008(T) | Recombinant Anti-human ERBB2 Intrabody [Tat] | ICC, Neut, FuncS | scFv-Tat |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-761 | Anti-ERBB2 Recombinant Antibody (Margetuximab) | IF, IP, Neut, FuncS, ELISA, FC, WB | IgG1 - kappa |
| TAB-049CT | Anti-Human HER2/neu Recombinant Antibody (ChA21) | IHC, Inhib, WB, Activ, FC | Chimeric antibody (mouse/human) |
| TAB-049CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (ChA21) | WB | Chimeric antibody (mouse/human) |
| TAB-049CT-F(E) | Anti-Human HER2/neu Recombinant Antibody Fab Fragment (ChA21) | WB | Chimeric antibody (mouse/human) |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G022E | Anti-ERBB2 immunotoxin 4D5 (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-G022D | Anti-ERBB2 immunotoxin 4D5 (scFv)-DT | Cytotoxicity assay, Function study | |
| AGTO-G022G | Anti-ERBB2 immunotoxin 4D5 (scFv)-Gel | Cytotoxicity assay, Function study | |
| AGTO-G022R | Anti-ERBB2 immunotoxin 4D5 (scFv)-RTA | Cytotoxicity assay, Function study | |
| AGTO-G022S | Anti-ERBB2 immunotoxin 4D5 (scFv)-Sap | Cytotoxicity assay, Function study |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-126 | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (PFBL-126) | ELISA, WB, FuncS | Human Fab |
| PFBC-089 | Mouse Anti-ERBB2 Recombinant Antibody (clone A21); Fab Fragment | ELISA, WB, FC | Mouse Fab |
| HPAB-0091-FY-F(E) | Human Anti-ERBB2 Recombinant Antibody (clone MIL12); Fab Fragment | ELISA | Humanized Fab |
| HPAB-M0510-YC-F(E) | Mouse Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-M0510-YC-F(E)) | FC, FuncS | Mouse Fab |
| HPAB-M0073-YC-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-M0073-YC-F(E)) | ELISA, FC | Human Fab |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-034CT | Anti-Human HER2/neu Recombinant Antibody (pertuzumab ) | WB | |
| TAB-036CT | Anti-Human HER2/neu Recombinant Antibody (6B2) | ELISA, WB | |
| TAB-037CT | Anti-Human HER2/neu Recombinant Antibody (7C2/7F3) | ELISA, WB | |
| TAB-034CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (pertuzumab ) | WB | |
| TAB-270CQ | Mouse Anti-ERBB2 Recombinant Antibody (TAB-270CQ) | WB, Inhib | Mouse IgG |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-054CT | Humanized Anti-Human HER2/neu Recombinant Antibody | ELISA, Inhibition, FC | Humanized antibody |
| TAB-041CT-S(P) | Human Anti-ERBB2 Recombinant Antibody; scFv Fragment (TAB-041CT-S(P)) | ELISA, WB | Humanized scFv |
| TAB-050CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (HuA21) | ELISA, WB | Humanized antibody |
| TAB-053CT-S(P) | Human Anti-ERBB2 Recombinant Antibody; scFv Fragment (TAB-053CT-S(P)) | ELISA, Inhib, FC | Humanized scFv |
| TAB-054CT-S(P) | Humanized Anti-Human HER2/neu Recombinant Antibody scFv Fragment | ELISA, Inhibition, FC | Humanized antibody |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-040CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (C6.5) | FC, IP, Block | Human antibody |
| TAB-042CT-F(E) | Anti-Human HER2/neu Recombinant Antibody Fab Fragment (CRX01) | WB | Human antibody |
| TAB-043CT-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (TAB-043CT-F(E)) | ELISA, Inhibion | Human Fab |
| TAB-055CT-F(E) | Anti-Human HER2/neu Recombinant Antibody Fab Fragment | WB | Human antibody |
| TAB-056CT-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (TAB-056CT-F(E)) | WB | Human Fab |
Fab Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-024LC | Recombinant Anti-Human ERBB2 Antibody (Fab glycosylation) | ELISA | Mouse antibody |
| Gly-039LC | Recombinant Anti-Human ERBB2 Antibody (Fab glycosylation) | ELISA | Human antibody |
Fc Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-117LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Humanized antibody |
| Gly-118LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Mouse antibody |
| Gly-145LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
| Gly-146LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
| Gly-147LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
Deglycosylated Antibody (Non-glycosylated IgGs)
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-177LC | Recombinant Anti-Human ERBB2 Antibody (Non-glycosylated) | ELISA | Humanized antibody |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0195MZ | Chicken Anti-ERBB2 Polyclonal IgY | WB | Chicken antibody |
| BRD-0695MZ | Chicken Anti-ERBB2 Polyclonal IgY | WB | Chicken antibody |
| BRD-0779MZ | Chicken Anti-ErbB2 Polyclonal IgY | ICC, IF, IHC, IP | Chicken antibody |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-737CQ | Mouse Anti-ERBB2 Recombinant Antibody (clone 7.16.4) | Block, IP, IF, FC | Mouse IgG2a |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1178 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone DS1178AB) | IHC-P, IHC-Fr | Rabbit IgG |
| MOR-4586 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone TH99DS) | WB | Rabbit IgG |
| MOR-4587 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone TH100DS) | WB | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0098YC | Mouse Anti-ERBB2 Recombinant Antibody (clone E402) | Cyt | Mouse IgG1, κ |
| FAMAB-0099YC | Human Anti-ERBB2 Recombinant Antibody (clone CH402) | Cyt | Chimeric (mouse/human) IgG1 |
| FAMAB-0132CQ | Mouse Anti-ERBB2 Recombinant Antibody (clone SER4) | ELISA | Mouse IgG1 |
| HPAB-1111LY | Mouse Anti-ERBB2 Recombinant Antibody (HPAB-1111LY) | IHC, FuncS, FC | Mouse IgG |
| HPAB-1112LY | Human Anti-ERBB2 Recombinant Antibody (HPAB-1112LY) | ELISA | Chimeric (Mouse/Human) IgG |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-468CQ | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Timigutuzumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| AFC-TAB-053 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Pertuzumab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
| AFC-TAB-761 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Margetuximab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-005 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Trastuzumab), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0641 | PE-A*02:01/Human ERBB2 (LIAHNQVRQV) MHC Tetramer | FCM, IHC | |
| MHC-CN0642 | APC-A*02:01/Human ERBB2 (LIAHNQVRQV) MHC Tetramer | FCM, IHC | |
| MHC-CN0643 | FITC-A*02:01/Human ERBB2 (LIAHNQVRQV) MHC Tetramer | FCM, IHC | |
| MHC-CN0644 | A*02:01/Human ERBB2 (LIAHNQVRQV) MHC Tetramer | FCM, IHC | |
| MHC-CN0645 | PE-A*02:01/Human ERBB2 (ILHDGAYSL) MHC Tetramer | FCM, IHC |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2957LY-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone P2h2); scfv Fragment | ELISA, FC | Humanized scfv |
| HPAB-S0027-YC-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone F5); scFv Fragment | ELISA, Inhib, Internalization | Human scFv |
| HPAB-S0028-YC-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone C1); scFv Fragment | ELISA, Inhib, Internalization | Human scFv |
| HPAB-1855-FY-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-1855-FY-F(E)) | ELISA | Humanized Fab |
| FAMAB-0359JF-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone FRc13); scFv Fragment | DB, ELISA, FuncS | Human scFv |
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