What format is this ERBB2 antibody?

This product is a Recombinant human antibody Fab Fragment. It recognizes HER2/neu (ERBB2) and is purified by affinity chromatography. It is suitable for ELISA, Inhibition, and Flow Cytometry.

Does this antibody bind to breast cancer cells?

Yes, this antibody binds to Human breast cancer cell lines such as AU565. It specifically targets the Human ERBB2 receptor tyrosine kinase and can be used to study overexpression in cancer models.

Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (TAB-053CT-F(E))

CAT#: TAB-053CT-F(E)

This product is a recombinant human antibody Fab Fragment that recognizes HER2/neu. The antibody was purified by affinity chromatography.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1060/169_D12_1_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1338/if_selected.jpg

Normal Tissue Figure 3 High expression in esophagus

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1383/4682_B_5_1_val_selected.jpg

Normal Tissue Figure 4 IHC staining of human rectum

Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/ihc_selected.jpg

Normal Tissue Figure 5 IHC staining of human urinary bladder

Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/ihc_selected.jpg

Normal Tissue Figure 6 Kidney

Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/1054_A_7_5.jpg

Normal Tissue Figure 7 Breast

Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/45831_B_3_4.jpg

RNA Expression Figure 8 RNA cell line category: Group enriched (OE19, SK-BR-3)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141736-ERBB2

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Specifications

Immunogen
Human breast cancer cell line AU565

Host Species
Human

Type
Humanized Fab

Specificity
Human

Species Reactivity
Human

Applications
Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay; Inhibition; Flow Cytometry

Related Disease
Gastric cancers, Breast cancers

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
ERBB2; erb-b2 receptor tyrosine kinase 2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu; receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; human epidermal growth factor receptor 2; neuro/glioblastoma derived oncogene homolog; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog;

Gene ID
2064

UniProt ID
P04626

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Breast cancer biomarkers at key points during disease progression

Specific Fab Fragment

This recombinant human Fab fragment against HER2/neu is highly specific. We used it in ELISA and Flow Cytometry, and it bound strongly to the extracellular domain of the receptor. The Fab format is particularly useful for our inhibition studies where we want to avoid Fc-mediated effects.

Excellent For Inhibition

We employed this antibody fragment in inhibition assays to study ERBB2 signaling. It effectively bound to the receptor on human breast cancer cell lines like AU565. It is a precise tool for investigating the dimerization and kinase activation pathways of the EGF receptor family.

Q&As

What format is this ERBB2 antibody?

A: This product is a Recombinant human antibody Fab Fragment. It recognizes HER2/neu (ERBB2) and is purified by affinity chromatography. It is suitable for ELISA, Inhibition, and Flow Cytometry.
Does this antibody bind to breast cancer cells?

A: Yes, this antibody binds to Human breast cancer cell lines such as AU565. It specifically targets the Human ERBB2 receptor tyrosine kinase and can be used to study overexpression in cancer models.

View the frequently asked questions answered by Creative Biolabs Support.

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Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.

Related Diseases

Bladder Cancer

Non-small Cell Lung Cancer

Related Signaling Pathways

ErbB Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-053CT-F(E).pdf

MSDS

MSDS - TAB-053CT-F(E).pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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