Mouse Anti-HGF Recombinant Antibody (clone 5D20)
CAT#: NEUT-901CQ
This antibody is a mouse monoclonal antibody that binds specifically to HGF, and it can neutralize the bioactivity of HGF.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40360/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40360/92262_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40360/92262_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40360/92262_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40360/92262_A_5_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced.
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000019991-HGF
❮
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Specifications
- Immunogen
- Recombinant human HGF
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human HGF
- Species Reactivity
- Human
- Clone
- 5D20
- Applications
- WB, Neut
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Neutralizes HGF Bioactivity
We were looking for a mouse monoclonal antibody capable of neutralizing HGF bioactivity for our cell motility assays. Clone 5D20 worked perfectly for this purpose, effectively inhibiting the cytokine's function in our in vitro models.
Verified By SDS-page
The quality of this recombinant antibody is top-notch. It was greater than 95% pure by SDS-PAGE, which is critical for our sensitive Western Blot applications. It binds specifically to human HGF without non-specific interactions.
Q&As
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Can this antibody neutralize the biological activity of HGF?
A: Yes, this mouse monoclonal antibody (clone 5D20) is capable of neutralizing the bioactivity of HGF. It binds specifically to the hepatocyte growth factor and is suitable for use in neutralization assays as well as Western Blotting.
-
What is the isotype of this mouse monoclonal antibody?
A: The isotype of this antibody is Mouse IgG1. It is a recombinant antibody expressed in human systems but retains the mouse IgG1 constant region properties, making it compatible with standard secondary antibodies targeting mouse IgG.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| TAB-080 | Human Anti-HGF Recombinant Antibody (TAB-080) | WB, ELISA, FC, IP, FuncS, IF, Neut | Human IgG2, κ |
| CAT | Product Name | Application | Type |
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| TAB-749 | Anti-HGF Recombinant Antibody (TAB-749) | ELISA, FC, IP, FuncS, IF, Neut, WB | IgG1 - kappa |
| CAT | Product Name | Application | Type |
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| PABL-132 | Rabbit Anti-HGF Recombinant Antibody (PABL-132) | WB, ELISA, FuncS | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| PSBL-132 | Rabbit Anti-HGF Recombinant Antibody; scFv Fragment (PSBL-132) | WB, ELISA, FuncS | Rabbit scFv |
| CAT | Product Name | Application | Type |
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| PFBL-132 | Rabbit Anti-HGF Recombinant Antibody; Fab Fragment (PFBL-132) | WB, ELISA, FuncS | Rabbit Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-075CT | Human Anti-HGF Recombinant Antibody (TAB-075CT) | ELISA, Neut | Humanized antibody |
| CAT | Product Name | Application | Type |
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| TAB-076CT | Anti-Human HGF/SF Recombinant Antibody (2C3) | ELISA, WB | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-075CT-S(P) | Human Anti-HGF Recombinant Antibody; scFv Fragment (TAB-075CT-S(P)) | ELISA, Neut | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-076CT-S(P) | Anti-Human HGF/SF Recombinant Antibody scFv Fragment (2C3) | ELISA, WB | Human antibody |
| CAT | Product Name | Application | Type |
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| TAB-075CT-F(E) | Human Anti-HGF Recombinant Antibody; Fab Fragment (TAB-075CT-F(E)) | ELISA, Neut | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-076CT-F(E) | Anti-Human HGF/SF Recombinant Antibody Fab Fragment (2C3) | ELISA, WB | Human antibody |
| CAT | Product Name | Application | Type |
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| TAB-078CT | Llama Anti-HGF Recombinant Single Domain Antibody (TAB-078CT) | Block | Llama VHH |
| CAT | Product Name | Application | Type |
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| MOB-0899CT | Recombinant Mouse anti-Human HGF Monoclonal antibody (0.O.437) | ELISA, IP, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-900CQ | Mouse Anti-HGF Recombinant Antibody (clone 24612.111) | ELISA, WB, Neut | Mouse IgG1 |
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| NEUT-902CQ | Mouse Anti-HGF Recombinant Antibody (clone CBL070) | Neut, WB | Mouse IgG1 |
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| NEUT-903CQ | Mouse Anti-HGF Recombinant Antibody (clone CBL435) | WB, Neut | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| NEUT-904CQ | Mouse Anti-HGF Recombinant Antibody (clone CBL1017) | Neut | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| MOR-1591 | Rabbit Anti-HGF Recombinant Antibody (clone DS1591AB) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| MOR-4217 | Rabbit Anti-HGF Recombinant Antibody (clone SI296DS) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| MOR-4585 | Rabbit Anti-HGF Recombinant Antibody (clone TH98DS) | WB, IF, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| HPAB-0651-CN | Mouse Anti-HGF Recombinant Antibody (HPAB-0651-CN) | IHC, WB, FC | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0652-CN | Mouse Anti-HGF Recombinant Antibody (HPAB-0652-CN) | ELISA, Neut | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0651-CN-S(P) | Mouse Anti-HGF Recombinant Antibody; scFv Fragment (HPAB-0651-CN-S(P)) | ELISA, Neut | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0652-CN-S(P) | Mouse Anti-HGF Recombinant Antibody; scFv Fragment (HPAB-0652-CN-S(P)) | ELISA, Neut | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0653-CN-S(P) | Mouse Anti-HGF Recombinant Antibody; scFv Fragment (HPAB-0653-CN-S(P)) | ELISA, Neut | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0654-CN-S(P) | Mouse Anti-HGF Recombinant Antibody; scFv Fragment (HPAB-0654-CN-S(P)) | ELISA, Neut | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0651-CN-F(E) | Mouse Anti-HGF Recombinant Antibody; Fab Fragment (HPAB-0651-CN-F(E)) | ELISA, Neut | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0652-CN-F(E) | Mouse Anti-HGF Recombinant Antibody; Fab Fragment (HPAB-0652-CN-F(E)) | ELISA, Neut | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0653-CN-F(E) | Mouse Anti-HGF Recombinant Antibody; Fab Fragment (HPAB-0653-CN-F(E)) | ELISA, Neut | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0654-CN-F(E) | Mouse Anti-HGF Recombinant Antibody; Fab Fragment (HPAB-0654-CN-F(E)) | ELISA, Neut | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-130 | Afuco™ Anti-HGF ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-130) | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-749 | Afuco™ Anti-HGF ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-749) | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-080 | Afuco™ Anti-HGF Recombinant Antibody (AFC-TAB-080), ADCC Enhanced | ELISA, FC, IP, FuncS, IF | Human IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0993WJ | Recombinant Camelid Anti-HGF Single Domain Antibody (HPAB-0993WJ) | ELISA | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0994WJ | Recombinant Human Anti-HGF Antibody (HPAB-0994WJ) | ELISA | Humanized sdAb |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1015 | Anti-HGF Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY25 | Human Anti-HGF (clone BPC2015) scFv-Fc Chimera | ELISA, Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3130 | Anti-Mouse HGF Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3129 | Anti-Human HGF Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC141 | Recombinant Anti-HGF Eliminating Antibody, pH-Sensitive (VS-0625-YC141) | Antigen-Sweeping In Vivo. | Human IgG2 kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC154 | SmartAb™ Recombinant Anti-HGF pH-dependent Antibody (VS-0825-YC154) | ELISA, FC, IP IF, Neut, WB | Human IgG1 kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1025-YC52 | Anti-HGF Antibody Prodrug, Protease Activated (VS-1025-YC52) | ISZ, Cyt, FuncS |
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