Rabbit Anti-HIF1A Recombinant Antibody (clone TH102DS)
CAT#: MOR-4589
Recombinant Rabbit anti-HIF1A monoclonal antibody for WB, IF, ICC, FCM, ChIP, ELISA, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/ihc_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17442/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_B_8_5.jpg
Normal Tissue
Figure 4 Gallbladder
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_A_5_4.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (LHCN-M2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100644-HIF1A
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HIF1A
- Species Reactivity
- Human
- Clone
- TH102DS
- Applications
- WB, IF, ICC, FC, ChIP, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.09% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- ChIP 1 µl
ELISA Assay-dependent
FC 1:100-1:500
ICC 1:100-1:500
IF 1:100-1:500
WB 1:500-1:5000
Target
- Alternative Names
- Hypoxia Inducible Factor 1 Alpha Subunit; Hypoxia Inducible Factor 1, Alpha Subunit (Basic Helix-Loop-Helix Transcription Factor); Class E Basic Helix-Loop-Helix Protein 78; Basic-Helix-Loop-Helix-PAS Protein MOP1; PAS Domain-Containing Protein 8; ARNT-Interacting Protein; Member Of PAS Protein 1; HIF-1-Alpha; HIF1-ALPHA; BHLHe78; PASD8; MOP1; Hypoxia-Inducible Factor 1, Alpha Subunit (Basic Helix-Loop-Helix Transcription Factor); Hypoxia-Inducible Factor 1 Alpha Isoform I.3; Hypoxia-Inducible Factor1alpha; Member Of PAS Superfamily 1; ARNT Interacting Protein; HIF-1alpha; HIF-1A; HIF1
- Gene ID
- 3091
- UniProt ID
- Q16665
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "HIF1A"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1828z | Mouse Anti-HIF1A Recombinant Antibody (clone 6F11) | WB, ELISA, IF | Mouse IgG1 |
| MRO-1928-CN | Rabbit Anti-HIF1A Polyclonal Antibody (MRO-1928-CN) | WB, IF, FC | Rabbit IgG |
| MRO-1929-CN | Rabbit Anti-HIF1A Polyclonal Antibody (MRO-1929-CN) | IF, IHC, FC | Rabbit IgG |
| VS3-XY804 | Mouse Anti-HIF1A Recombinant Antibody (clone 5B1) | IHC | |
| VS3-XY805 | Mouse Anti-HIF1A Recombinant Antibody (clone 1A3) | ELISA, WB, IHC, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-080CT | Anti-Human HIF-1alpha Recombinant Antibody (6A9) | FuncS | |
| TAB-081CT | Anti-Human HIF-1alpha Recombinant Antibody (1H1) | WB | |
| TAB-082CT | Anti-Human HIF-1alpha Recombinant Antibody (6H4) | WB | |
| TAB-083CT | Anti-Human HIF-1alpha Recombinant Antibody (5A5) | WB, Inhibit | |
| TAB-084CT | Anti-Human HIF-1alpha Recombinant Antibody (7D6) | WB, Inhibit |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1595 | Rabbit Anti-HIF1A Recombinant Antibody (clone DS1595AB) | IHC, WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY248 | Mouse Anti-NHP HIF1A Recombinant Antibody (clone H1alpha67) | WB, IHC, IP, ELISA | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3136 | Anti-HIF1A Immunohistochemistry Kit | IHC | |
| VS-0525-XY3137 | Anti-Mouse HIF1A Immunohistochemistry Kit | IHC | |
| VS-0525-XY3138 | Anti-Porcine HIF1A Immunohistochemistry Kit | IHC | |
| VS-0525-XY3139 | Anti-Rabbit HIF1A Immunohistochemistry Kit | IHC |
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