Human Anti-HIV-1 gp120 Recombinant Antibody (clone 447-52D) (CAT#: PABX-088)

Anti-HIV-1 antibody is a Human antibody of IgG3 λ class that binds to an HIV-1. This antibody recognizes the hypervariable gp120 V3 loop, and neutralizes both X4 and R5 primary isolates, making it one of the most effective anti-V3 antibodies characterized to date.


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Figure 1 Binding of the mutated 447-52D IgG to the indicated gp140 trimers as measured by ELISA.

Figure 1 Binding of the mutated 447-52D IgG to the indicated gp140 trimers as measured by ELISA.

McGuire, A. T., Glenn, J. A., Lippy, A., & Stamatatos, L. (2014). Diverse recombinant HIV-1 Envs fail to activate B cells expressing the germline B cell receptors of the broadly neutralizing anti-HIV-1 antibodies PG9 and 447-52D. Journal of virology, 88(5), 2645-2657.

Figure 2 The area under the curve of the Ca2 flux response (determined in panel C) through the mutated 447-52D BCR correlates with the affinity of sIgG 447-52D (determined in panel A).

Figure 2 The area under the curve of the Ca2 flux response (determined in panel C) through the mutated 447-52D BCR correlates with the affinity of sIgG 447-52D (determined in panel A).

McGuire, A. T., Glenn, J. A., Lippy, A., & Stamatatos, L. (2014). Diverse recombinant HIV-1 Envs fail to activate B cells expressing the germline B cell receptors of the broadly neutralizing anti-HIV-1 antibodies PG9 and 447-52D. Journal of virology, 88(5), 2645-2657.

Figure 3 Neutralization of chimeric psVs by antibodies 447-52D (purple) and 2219 (blue).

Figure 3 Neutralization of chimeric psVs by antibodies 447-52D (purple) and 2219 (blue).

The psV consist of the SF162 strain with its V3 loop replaced by the indicated V3 loop sequence. Consensus B-R18Q is a psV consisting of the consensus subtype B V3 loop sequence with position 18 in the V3 loop mutated from a subtype B consensus arginine to a subtype C consensus glutamine. IC50 is the amount of antibody in ug/mL required to achieve 50% neutralization.
The negative base 10 logarithm of the IC50 has been plotted
for easier comparison: higher, positive bars towards the top of the
graph indicate strong neutralization by the antibody. Antibodies
were only tested to a concentration of 20 ug/mL for neutralization,
so the negative value of 1.3 on the plot is maximal and indicated no
detectable neutralization by the antibody

Almond, D., Krachmarov, C., Swetnam, J., Zolla-Pazner, S., & Cardozo, T. (2012). Resistance of subtype C HIV-1 strains to anti-V3 loop antibodies. Advances in virology, 2012.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Human
  • Type
  • Human IgG3, λ
  • Specificity
  • Tested positive against HIV-1
  • Species Reactivity
  • HIV-1
  • Clone
  • 447-52D
  • Applications
  • WB 1:1000-1:5000
    ELISA 1:3000-1:15000
    IF 1:100 -1:500
    FuncS (assay dependent)

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Assay-dependent

Target

  • Alternative Names
  • HIV-1; human immunodeficiency virus

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "HIV-1 gp120"

Single-domain Antibody

Chimeric Antibody

CAT Product Name Application Type
MRO-135LC Anti-HIV-1 gp120 Recombinant Antibody (CAG1-51-4) ELISA, WB, Neut Chimeric antibody (mouse/human)
MRO-135LC-S(P) Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (CAG1-51-4) ELISA, WB Chimeric antibody (mouse/human)
MRO-135LC-F(E) Anti-HIV-1 gp120 Recombinant Antibody Fab Fragment (CAG1-51-4) ELISA, WB Chimeric antibody (mouse/human)

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-4220 Rabbit Anti-HIV-1 gp120 Recombinant Antibody (clone SI299DS) WB, ELISA Rabbit IgG

Humanized Antibody

CAT Product Name Application Type
PABX-101-S(P) Human Anti-HIV-1 gp120 Recombinant Antibody (clone PG9); scFv Fragment WB, ELISA, FuncS Human scFv

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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